The partial denitrification (NO3--N→NO2--N), a promising way for nitrate wastewater treatment, could combine with ANAMMOX technology to achieve nitrogen removal with low concentration of carbon source and poor energy. Three different domestication pattern, R1 (SBR under anoxic condition), R2 (SBR under anoxia-aerobic alternating condition) and R3 (SBR under anoxic condition with low-intensity aeration), were conducted using glucose as electron donor to find out the rule of nitrite accumulation in denitrification process. These three reactors achieved steady nitrite accumulation after 120-days run, and the sludge were taken, respectively, to explore the denitrification pattern of these three acclimatizing ways. The findings revealed that, compared with R1, the R2 and R3 sludge could better achieve partial denitrification due to the involving of dissolved oxygen. And R3 was the best way. The microbial community structure of R1, R2 and R3 sludges on the 109th day were analyzed and compared by high throughput sequencing. The results showed that the dominant bacteria were Candidatus Saccharibacteria in both R2 and R3 reactor, which were with the participation of dissolved oxygen, and the relative abundance was 45.44% and 34.96%, respectively. This was the first time that the Candidatus Saccharibacteria was reported as the dominant bacteria in the denitrifying sludge. Besides, the microbial diversity of R1reactor was much larger than that of R2 and R3, which indicated that the denitrifying bacteria in R2 and R3 reactors were more exclusive. The batch experiments showed that the initial pH had a significant effect on the accumulation of nitrite. The higher the pH indicated the higher accumulation rate of nitrite.
王维奇, 王秀杰, 李军, 王思宇. 不同驯化方式实现SBR中部分反硝化的对比研究[J]. 中国环境科学, 2018, 38(11): 4085-4093.
WANG Wei-qi, WANG Xiu-jie, LI Jun, WANG Si-yu. Comparative study on achieving partial denitrification in a SBR by different acclimation methods. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(11): 4085-4093.
Oh J, Silverstein J A. Acetate limitation and nitrite accumulation during denitrification[J]. Journal of Environmental Engineering, 1999,125(3):234-242.
Xu B, Enfors S O. Modeling of nitrite accumulation by the denitrifying bacterium Pseudomonas stutzeri[J]. Journal of fermentation and bioengineering, 1996,82(1):56-60.
Hongwei S, Qing Y, Yongzhen P, et al. Nitrite accumulation during the denitrification process in SBR for the treatment of pre-treated landfill leachate[J]. Chinese Journal of Chemical Engineering, 2009,17(6):1027-1031.
Kalyuzhnyi S, Gladchenko M, Mulder A, et al. DEAMOX-New biological nitrogen removal process based on anaerobic ammonia oxidation coupled to sulphide-driven conversion of nitrate into nitrite[J]. Water Research, 2006,40(19):3637-3645.
Cao S, Wang S, Peng Y, et al. Achieving partial denitrification with sludge fermentation liquid as carbon source:The effect of seeding sludge[J]. Bioresour Technol, 2013,149(4):570-574.
Lee D Y, Ramos A, Macomber L, et al. Taxis response of various denitrifying bacteria to nitrate and nitrite[J]. Applied and environmental microbiology, 2002,68(5):2140-2147.
Tavares P, Pereira A S, Moura J J G, et al. Metalloenzymes of the denitrification pathway[J]. Journal of inorganic biochemistry, 2006, 100(12):2087-2100.
Metcalfeddy I, Tchobanoglous G, Stensel H D. Wastewater engineering:treatment and reuse[J]. McGraw-Hill Series in Water Resources and Environmental Engineering, 2007,73(1):50-51.
Ge S, Peng Y, Wang S, et al. Nitrite accumulation under constant temperature in anoxic denitrification process:The effects of carbon sources and COD/NO(3)-N.[J]. Bioresource Technology, 2012,114(3):137-143.
刘琦.不同碳源下反硝化过程中亚硝酸盐积累规律研究[D]. 天津:天津大学, 2015.
李杰.吡啶缺氧降解动力学与过程控制因素研究[D]. 上海:同济大学, 2006.
杜晓娜.反硝化过程中亚硝酸盐积累影响因素与稳定运行[D]. 天津:天津大学, 2016.
Glass C, Silverstein J A. Denitrification kinetics of high nitrate concentration water:pH effect on inhibition and nitrite accumulation[J]. Water Research Oxford, 1998,32(3):831-839.
Shahabi Z A, Naeimpoor F. Enhanced Heterotrophic Denitrification:Effect of Dairy Industry Sludge Acclimatization and Operating Conditions[J]. Applied Biochemistry & Biotechnology, 2014,173(3):741-752.
Sumino T, Isaka K, Ikuta H, et al. Nitrogen removal from wastewater using simultaneous nitrate reduction and anaerobic ammonium oxidation in single reactor[J]. Journal of bioscience and bioengineering, 2006,102(4):346-351.
Yang X, Wang S, Zhou L. Effect of carbon source, C/N ratio, nitrate and dissolved oxygen concentration on nitrite and ammonium production from denitrification process by Pseudomonas stutzeri D6[J]. Bioresource Technology, 2012,104:65-72.
Li L, Dong Y, Qian G, et al. Performance and microbial community analysis of bio-electrocoagulation on simultaneous nitrification and denitrification in submerged membrane bioreactor at limited dissolved oxygen[J]. Bioresour Technol, 2018,258:168-176.
Kindaichi T, Yamaoka S, Uehara R, et al. Phylogenetic Diversity and Ecophysiology of Candidate Phylum Saccharibacteria in Activated Sludge[J]. Fems Microbiology Ecology, 2016,92(6):fiw078.
Huber K J, Geppert A M, Groß U, et al. Aridibacter nitratireducens sp. nov. a member of the family Blastocatellaceae, class Blastocatellia, isolated from an African soil[J]. International Journal of Systematic & Evolutionary Microbiology, 2017,67(11).
Lv P, Luo J, Zhuang X, et al. Diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in Liangshui River of Beijing, China[J]. Scientific Reports, 2017,7(1):10032.
Li B, Pan X, Zhang D, et al. Anaerobic nitrate reduction with oxidation of Fe(Ⅱ) by Citrobacter Freundii, strain PXL1-a potential candidate for simultaneous removal of As and nitrate from groundwater[J]. Ecological Engineering, 2015,77(1):196-201.
Rui D, Cao S, Li B, et al. Performance and microbial community analysis of a novel DEAMOX based on partial-denitrification and anammox treating ammonia and nitrate wastewaters[J]. Water Research, 2016,108:46-56.
Starr E P, Shi S, Blazewicz S J, et al. Stable isotope informed genome-resolved metagenomics reveals that Saccharibacteria utilize microbially-processed plant-derived carbon[J]. Microbiome, 2018, 6(1):122.
Remmas N, Melidis P, Zerva I, et al. Dominance of candidate Saccharibacteria in a membrane bioreactor treating medium age landfill leachate:Effects of organic load on microbial communities, hydrolytic potential and extracellular polymeric substances[J]. Bioresource technology, 2017,238:48-56.
Zhao J, Li Y, Chen X, et al. Effects of carbon sources on sludge performance and microbial community for 4-chlorophenol wastewater treatment in sequencing batch reactors[J]. Bioresource technology, 2018,255:22-28.