Bioremediation of petroleum contaminated soil by microoganisms enhanced with spent mushroom substrate
ZHANG Bo-fan1, XIONG Xin1, HAN Zhuo2, ZHANG Xiu-xia1, LIU Ze-yang1, MA Nian1, LIU Hui-e1, GU Ying-ying1
1. College of Chemical Engineering, China University of Petroleum(East China), Qingdao 266580, China; 2. Technical Detection Center of Shengli Oilfield Branch, Dongying 257000, China
Abstract：The spent mushroom substrate (SS) and Microbacterium.sp.Q2 bacteria were used to remediate petroleum-contaminated soil and four different treatments which contained spent mushroom substrate immobilized microorganisms (SIM), spent mushroom substrate-free bacteria (SMSB), spent mushroom substrate alone (SMS) and no treatment (CK) were designed in this paper. The aim of this study is to investigate soil microbial number, enzyme activities and the degradation rate of total petroleum hydrocarbon (TPH), exploring an ideal reparative treatment. The results indicated that soil respiration intensity, microbial number and enzyme activities of SIM group were obviously higher than others and the removal rate of TPH was highest which was increased by 11.84%, 22.15% and 54.09% compared with other treatments. Furthermore it is showed that the dehydrogenase activity and microbial activity were significantly correlated with the degradation rate of TPH and immobilized microorganism had the synergistic mechanism of bioaugmentation and biostimulation for remediation of petroleum contaminated soil.
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