The bioaccumulation and metabolic kinetics of climbazole in fish
YAO Li1,3, MA Yan-fang1, ZHOU Yi-bo1, DOU Wen-yuan1, JIA Yu-wei2, ZHAO Jian-liang2, YING Guang-guo2
1. Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemical, China National Analytical Center(Guangzhou), Guangzhou 510070, China; 2. Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety, the Environmental Research Institute, South China Normal University, Guangzhou 510006, China; 3. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
Abstract：We used a typical fish species in South China, tilapia, to investigate the uptake and elimination kinetics of climbazole in fish gill, liver, bile and plasma following a 7-d exposure to aqueous solution of climbazole at the concentration of 2.00ng/mL. We also compared the uptake and elimination rates of climbazole in fish tissues. In the uptake process, the climbazole concentration reached the maximum after 3days of exposure, at which point the concentration in fish gill, liver, bile and plasma were up to 2.91ng/g, 33.7ng/g, 4.84ng/mL and 2.58ng/mL, respectively. In the elimination process, the climbazole was nearly eliminated in fish after 3days, with the climbazole concentrations lower than MQLs in fish gill, bile and plasma, and of 1.21ng/g in fish liver. The uptake kinetic constants (ku) of climbazole in fish gill, liver, bile and plasma were 0.069, 0.813, 0.286 and 0.136h-1, respectively; while the corresponding elimination kinetic constants (ke) were 0.033, 0.029, 0.082 and 0.060h-1, respectively. The half-time values of climbazole in fish gill, liver, bile and plasma were 21.1, 23.9, 8.51 and 11.6h, respectively. The uptake and elimination processes of climbazole in fish tissues followed the pseudo first-order kinetic model, with the correlation coefficients r2 in the range of 0.75~0.98. The log bioconcentration factor of climbazole at steady state in fish gill, liver, bile and plasma were 0.50, 1.56, 0.72 and 0.45, respectively, which were lower than those in wild tilapia fish.
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