Effect of extracellular polymers and signal molecules on the activity of ANAMMOX sludge
ZHANG Ya-chao1, ZHANG Jing1, HOU Ai-yue2, ZHOU Rong-xuan1, LIANG Dong-bo1, ZHANG Kai1, LIU Yang1, LI Jun1
1. College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China;
2. CAUPD Beijing Planning & Design Consultants Co., Beijing 100825, China
In order to investigate the effect of extracellular polymers (EPS) on the activity of anaerobic ammonium oxidation (ANAMMOX) granular sludge, a certain concentration (30mg/L) of integral extracellular polymers (IN-EPS), loosely bound extracellular polymers (LB-EPS) and tightly bound extracellular polymers (TB-EPS) were added to ANAMMOX granular sludge, respectively. The results indicated that all of the aforementioned EPS could improve the activity of ANAMMOX granular sludge. With regard to the test group including IN-EPS, LB-EPS or TB-EPS, the ammonia nitrogen removal rate of ANAMMOX granular sludge had an increase of 6.68%, 10.5% and 19.29% respectively, while the growth rate of ANAMMOX bacteria increased by 18.25%, 21% and 16.3% respectively. The compositions of three EPSs were basically identical, which is mainly consisted of aromatic proteins and soluble microbial products through three-dimensional fluorescence spectroscopy. And three kinds of acylated homoserine lactones (AHLs) signal molecules in EPSs:C4-HSL, C6-HSL and C10-HSL were found. Additionally, we discovered that C4-HSL and C6-HSL could improve the activity and growth rate of ANAMMOX particles through adding the above EPSs to ANAMMOX granular sludge. This interpreted why EPS could improve the activity of ANAMMOX granular sludge.
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