An aerobic methane oxidation coupled to denitrification (AME-D) was constructed using a gas circulation sequencing batch biofilm reactor (gcSBBR), and nitrogen loading in influent was observed. It was found that the removal rate of nitrate nitrogen reached 98.93% and the denitrification rate 74.25mg/(L·d) when the nitrogen load was 0.075kg/(m3·d), and the average daily consumption of methane was 35.91% (at the initial stage:50%). According to scanning electron microscopy (SEM) results, microorganisms in the system were mainly Bacillus brevis (12~18μm), dotted with a few filamentous bacteria (150~200μm). The high-throughput sequencing of 16S rRNA indicated that methanotroph were Methylocaldum, Methylomonas, Methylococcus and Methylococcaceae_unclassified, the denitrifying bacteria Denittrasoma, Hydrogenophaga, Azoarcus, Thiobacillus and Rhodobacter, and the main functional bacteria Methylocaldum, Denittrasoma and Hydrogenophaga in this system. Nitrogen was removed by the synergism of aerobic methanotroph and denitrifying bacteria. In addition, a large volume of Methylophilaceae_uncultured (30.4%), which utilized methanol and methylamine as growth substrates, was found.
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LI Yan-cheng, YANG Ya-nan, LIU Deng-ping, LI Lei, LI Jiang. Denitrification efficiency and microbial community research in an aerobic methanotroph-based system. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(10): 4387-4393.
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