Identification and characterization on PAHs-degrading microorganisms via cultivation-independent approaches
CHEN Ya-ting, JIANG Bo, XING Yi, ZHANG Na-na, LIAN Lu-ning, LU Pei
Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, School of Energy and Environmental Engineering, University of Science & Technology Beijing, Beijing 100083, China
This review discussed the cultivation-independent approaches which have been developed to identify functional-yet-uncultivated soil microbes in situ and revealed their functions in PAHs degradation. PAHs-degrading microbes, degradation mechanisms and functional genes were summarized. The principles, characteristics and applications of cultivation-independent methods, including Fluorescence in situ hybridization (FISH), Stable-isotope probing (SIP), Metagenomics, Transcriptomics, Single-cell technology and Magnetic nanoparticle-Mediated Isolation technology (MMI) were also discussed. Examples were given on the application of these cultivation-independent approaches in identifying PAHs-degrading microbes. SIP technology is the most stable and widely used in the study of PAHs degradation mechanism. As a novel cultivation-independent approach, MMI technology provides a deep insight into exploring functional-yet-uncultivated PAHs-degrading microbes, which stands out for its ability to separate active functional microbes.
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