Characteristics of bacterial community structure during the enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria based on the typical city landscape water
ZHOU Shi-lei1, ZHANG Yi-ran1, SUN Yue1, YANG Wen-li1, HUANG Ting-lin2, LI Zai-xing1, LUO Xiao1, CUI Jian-sheng1, ZHOU Zi-zhen3, LI Yang3
1. Pollution Prevention Biotechnology Laboratory of Hebei Province, School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; 2. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; 3. School of Energy and Environment, Zhongyuan University of Technology, Zhengzhou 450007, China
Abstract：To explore the effects of different selective pressures on bacterial community structure during enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria, bioinformatics analysis of samples taken from enrichment and domestication systems were carried out, using Miseq high-throughput sequencing. In detail, α-and β-diversity were examined, and network analysis was conducted. Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Actinobacteria, Cyanobacteria, and Acidobacteria were the main phyla identified. Meanwhile, the N-functional bacteria had an increased process. PCA (principal component analysis), NMDS (non-metric multidimensional scaling analysis) and PCoA (principal co-ordinates analysis) showed that microbial community structure was significantly altered with change in temperature, while the influence of different media was small. Network analysis indicated that module hubs and network hubs of bacterial communities were both rare taxa. VIF (variance inflation factor) and RDA (redundancy analysis) showed temperature, ammonia and nitrate were the most important factors affecting bacterial community function and composition. All results together indicate that Miseq high-throughput sequencing was an effective tool to explore changes in bacterial community structure during enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria, which could in the future supply a reference to isolate "directional-accurate-efficient" microbial agents.
周石磊, 张艺冉, 孙悦, 杨文丽, 黄廷林, 李再兴, 罗晓, 崔建升, 周子振, 李扬. 异养硝化-好氧反硝化菌富集驯化过程中微生物种群演变特征——典型城市景观水系[J]. 中国环境科学, 2019, 39(11): 4831-4839.
ZHOU Shi-lei, ZHANG Yi-ran, SUN Yue, YANG Wen-li, HUANG Ting-lin, LI Zai-xing, LUO Xiao, CUI Jian-sheng, ZHOU Zi-zhen, LI Yang. Characteristics of bacterial community structure during the enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria based on the typical city landscape water. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(11): 4831-4839.
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