Effects of pig manure on bacterial community in soils with long-term application of chemical fertilizer or manure
PENG Shuang1,2, LIN Xian-gui2, WANG Yi-ming2
1. College of Environment and Ecology, Jiangsu Open University, Nanjing 210017, China;
2. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
In order to explore the effect of bacteria carried by manure on soil microorganisms, and to determine whether the history of manure application has affected the response of soil microorganisms to manure application. Taking the soil with long-term application of pig manure and long-term application of chemical fertilizer as the research objects, manure and sterilized manure were added to these soils. CFU number of the culturable bacteria, abundance of 16S-rRNA gene, bacterial community composition and microbial thermal metabolic activity were measured. When pig manure was applied as 9.0t/hm2, the fecal bacteria and chemical nutrients carried by manure had no significant effect on the CFU number of culturable bacteria and 16S-rRNA copies in both soils. In addition, the soil indigenous microorganisms could significantly inhibit the colonization of exogenous bacteria carried by manure; if the indigenous microorganisms in these two soils had sterilized and pig manure was added, the average number of culturable bacteria would increase by 45 and 96 times respectively. Nutrients input from manure did not cause significant changes in soil bacterial community structure and abundance; while exogenous fecal bacteria imported from manure could colonize in the soil with long-term application of chemical fertilizer and affect the corresponding bacterial abundance. However, less fecal bacteria colonize in the soil with long-term application of pig manure, they were inhibited in the early stages of soil entry. Similarly, the addition of manure increased the microbial metabolic activity of the long-term chemical fertilizer application soil, but had no significant effect on the long-term manure application soil. In summary, manure has a greater impact on bacteria community in the soil with long-term application of chemical fertilizer, while the history of long-term application of manure reduces the "sensitivity" of the soil to manure, and enhances the inhibitory effect on the exogenous bacteria carried by manure.
彭双, 林先贵, 王一明. 添加猪粪对不同施肥历史土壤细菌群落的影响[J]. 中国环境科学, 2020, 40(2): 748-756.
PENG Shuang, LIN Xian-gui, WANG Yi-ming. Effects of pig manure on bacterial community in soils with long-term application of chemical fertilizer or manure. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(2): 748-756.
周贝贝,王一明,林先贵.不同处理方式的粪肥对水稻生长和温室气体排放的影响[J].应用与环境生物学报, 2016,22(3):0430-0436. Zhou B B, Wang Y M, Lin X G. Effects of differently treated manures on rice growth and greenhouse gas emission[J]. Chin J Appl Environ Biol, 2016,22(3):0430-0436.
[2]
陆雅海.土壤微生物学研究现状与展望[J].中国科学院院刊, 2015,30:106-114. Lu Y H. Recent development of soil microbiology and future perspectives[J]. Bulletin of Chinese Academy of Science, 2015,30:106-114.
[3]
Sharma S K, Ramesh A, Sharma M P, et al. Microbial community structure and diversity as indicators for evaluating soil quality[Z]. Biodiversity, Biofuels, Agroforestry and Conservation Agriculture. Edited by Lichtfouse E. Dordrecht:Springer Netherlands, 2011:317-358.
[4]
Tian W, Wang L, Li Y, et al. Responses of microbial activity, abundance, and community in wheat soil after three years of heavy fertilization with manure-based compost and inorganic nitrogen[J]. Agriculture, Ecosystems&Environment, 2015,213:219-227.
[5]
Hou S, Xin M, Wang L, et al. The effects of erosion on the microbial populations and enzyme activity in black soil of northeastern China[J]. Acta Ecol. Sin. 2014,34:295-301.
[6]
Ding J, Jiang X, Ma M, et al. Effect of 35 years inorganic fertilizer and manure amendment on structure of bacterial and archaeal communities in black soil of northeast China[J]. Applied Soil Ecology, 2016,105:187-195.
[7]
Wei D, Yang Q, Zhang J Z, et al. Bacterial community structure and diversity in a black soil as affected by long-term fertilization[J]. Pedosphere, 2008,18:582-592.
[8]
Urra J, Alkorta I, Lanzén A, et al. The application of fresh and composted horse and chicken manure affects soil quality, microbial composition and antibiotic resistance[J]. Applied Soil Ecology, 2019,135:73-84.
[9]
李江涛,钟晓兰,刘勤,等.长期施用畜禽粪便对土壤生物化学质量指标的影响[J].土壤, 2010,42(4):526-535. Li J T, Zhong X L, Liu Q, et al. Effects of long-term application of livestock manures on soil biochemical quality indicators[J]. Soils, 2010,42(4):526-535.
[10]
李小嘉,李江涛,倪杰,等.集约化畜禽粪便施用对土壤微生物群落功能多样性的影响[J].广东农业科学, 2014,6:193-198. Li X J, Li J T, Ni J, et al. Changes in functional diversity of soil microbial community after long-term application of intensive livestock and poultry manure[J]. Guangdong Agricultural Sciences, 2014,6:193-198.
[11]
Sun R, Zhang X X, Guo X, et al. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw[J]. Soil Biology and Biochemistry, 2015,88:9-18.
[12]
Chaudhry V, Rehman A, Mishra A, et al. Changes in bacterial community structure of agricultural land due to long-term organic and chemical amendments[J]. Microbial Ecology, 2012,64:450-460.
[13]
Wang Q, Jiang X, Guan D, et al. Long-term fertilization changes bacterial diversity and bacterial communities in the maize rhizosphere of Chinese Mollisols[J]. Applied Soil Ecology, 2018,125:88-96.
[14]
Han X M, Hu H W, Chen Q L, et al. Antibiotic resistance genes and associated bacterial communities in agricultural soils amended with different sources of animal manures[J]. Soil Biology and Biochemistry, 2018,126:91-102.
[15]
Biddle J F, Fitz-Gibbon S, Schuster S C, et al. Metagenomic signatures of the Peru Margin subseafloor biosphere show a genetically distinct environment[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008,105:10583-10588.
[16]
Peng S, Zhou B, Wang Y, et al. Bacteria play a more important role than nutrients in the accumulation of tetracycline resistance in manure-treated soil[J]. Biology and Fertility of Soils, 2016,52(5):655-663.
[17]
徐江兵,王艳玲,刘明,等. BIOLOG与微量热耦合研究提高红壤微生物代谢活性的碳源因子[J].土壤学报, 2018,55(1):203-212. Xu J B, Wang Y L, Liu M, et al. Use of BIOLOG and microcalorimetry in combination to study factors of carbon sources stimulating metabolic activity of soil microbe in red soil[J]. Acta Pedologica Sinica, 2018,55(1):203-212.
[18]
谭秋锦,宋同清,彭晚霞,等.峡谷型喀斯特不同生态系统的土壤微生物数量及生物量特征[J].生态学报, 2014,34(12):3302-3310. Tan Q J, Song T Q, Peng W X, et al. Characteristics of soil microbial populations and biomass under different ecosystems in a canyon karst region[J]. Acta Ecologica Sinica, 2014,34(12):3302-3310.
[19]
Wagg C, Bender S F, Widmer F, et al. Soil biodiversity and soil community composition determine ecosystem multifunctionality[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014,111:5266-5270.
[20]
van Elsas J D, Chiurazzi M, Mallon C A, et al. Microbial diversity determines the invasion of soil by a bacterial pathogen[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012,109(4):1159-1164.
[21]
Amann R I, Ludwig W, Schleifer K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiological Reviews, 1995,59:143-169.
[22]
张凯煜,谷洁,王小娟,等.微生物有机肥对樱桃园土壤细菌群落的影响[J].中国环境科学, 2019,39(3):1245-1252. Zhang K Y, Gu J, Wang X J, et al. Effects of bio-organic fertilizer on the soil bacterial community in a cherry orchard[J]. China Environmental Science, 2019,39(3):1245-1252.
[23]
杨亚东,王志敏,曾昭海.长期施肥和灌溉对土壤细菌数量、多样性和群落结构的影响[J].中国农业科学, 2018,51(2):290-301. Yang Y D, Wang Z M, Zeng Z H. Effects of long-term different fertilization and irrigation managements on soil bacterial abundance, diversity and composition[J]. Scientia Agricultura Sinica, 2018,51(2):290-301.
[24]
Chen Q L, An X L, Li H, et al. Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil?[J]. Soil Biology and Biochemistry, 2017,114:229-237.
[25]
李文,叶旭红,韩冰,等.不同灌溉方式下3种土壤微生物活性测定方法比较[J].生态学报, 2017,37(12):4084-4090. Li W, Ye X H, Han B, et al. Comparison of three methods used to determine soil microbial activity under different irrigation systems[J]. Acta Ecologica Sinica, 2017,37(12):4084-4090.
[26]
姜兰兰,刘燕,郑世学.等温微量热技术在土壤微生物学研究中的应用[J].应用与环境生物学报, 2016,22(4):0732-0738. Jiang L L, Liu Y, Zheng S X. Applications of isothermal microcalorimetry in study on soil microbial activity[J]. Chin J Appl Environ Biol, 2016,22(4):0732-0738.
[27]
郭莹,王一明,巫攀,等.长期施用粪肥对水稻土中微生物群落功能多样性的影响[J].应用与环境生物学报, 2019,25(3):593-602. Guo Y, Wang Y M, Wu P, et al. Influence of long-term manure application in paddy soil on functional diversity of microbial community[J]. Chin J Appl Environ Biol, 2019,25(3):593-602.
