Nitrate reduction coupled with As(III) oxidation process and community structure analysis in flooded paddy soil
LI Shuang, AO Jun-hua, WANG Qing, CHEN Di-wen, ZHOU Wen-ling, WU Qi-hua
Guangdong Provincial Bioengineering Institute(Guangzhou Sugarcane Industry Research Institute), Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou 510316, China
Paddy soil was collected from Southern China and used as an inoculum in a microcosm experiment. The microcosm experiment was used to study the process of autotrophic nitrate reduction coupled with As(III) oxidation and its microbial community structure in the flooded paddy field. As(III) was completely oxidized in As(III)+NO3- treatment, while no As(III) oxidation was observed in the As(III) amendment and the sterilization treatment (Sterilized soil+As(III)+NO3-). Meanwhile, a small amount of NO3- reduction was observed in As(III)+NO3- amendment and no NO3- reduction was observed in NO3- amendment. 16S rRNA-based high-throughput sequencing was used to investigate the microbial community structure in Soil+As(III)+NO3- treatment. Diversity of soil bacterial community was decreased and its Shannon index was 8.19in Soil+As(III)+NO3- treatment. The dominant bacteria at the phyla level were Proteobacteria (33%), Chloroflexi (11%), and Planctomycetes (12%), and the dominant genus were Gemmatimonas (7.4%), with few Singulisphaera、Thermomonas、Bacillus in Soil+As(III)+NO3- treatment. In summary, the addition of NO3- can promote the autotrophic As(III) oxidation and influences the microbial community composition in flooded paddy soil.
李爽, 敖俊华, 王庆, 陈迪文, 周文灵, 吴启华. 稻田NO3-还原耦合As(III)氧化过程及微生物群落特征[J]. 中国环境科学, 2020, 40(2): 757-763.
LI Shuang, AO Jun-hua, WANG Qing, CHEN Di-wen, ZHOU Wen-ling, WU Qi-hua. Nitrate reduction coupled with As(III) oxidation process and community structure analysis in flooded paddy soil. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(2): 757-763.
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