低DO对活性污泥系统碳和氮去除影响的研究

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摘要

考察了长期低溶解氧（DO）条件下推流式活性污泥系统碳和氮去除的效果，并从微生物群落结构及动力学特征方面进行了机理分析. 结果表明，将DO浓度从2.0mg/L降低至0.3mg/L，系统仍可有效地去除污水中的碳和氮，但当DO降低至0.3mg/L时，系统的脱氮性能波动较大. 随着DO浓度的降低，系统中细菌的总体群落结构发生改变，但功能菌群仍为Proteobacteria，约占65%.Nitrosomonas oligotropha、Nitrobacter winogradskyi spp.和Group1Nitrospira等是低溶解氧条件下的主要硝化细菌. qPCR结果显示DO从2.0mg/L降至0.5mg/L时硝化细菌（主要是Nitrospira）得到富集，保证了低DO条件下的完全硝化. 硝化反应动力学分析也表明，在一定范围内降低DO可以延迟细菌衰亡以维持活性污泥系统中硝化细菌的生物量. 本研究可为污水处理厂降低DO实现节能运行提供理论支持.

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	刘国华
	刘禹琛
	陈燕
	章乾
	徐相龙
	齐鲁
	王洪臣

关键词 ：低DO, 节能硝化, A/O工艺, 细菌群落, 推流式活性污泥系统

Abstract：

Impact of dissolved oxygen (DO) on pollutant removal and bacterial community structure was evaluated in a plug-flow activated sludge system. Results show that the bioprocess could still maintain high removal efficiencies of chemical oxygen demand and nitrogen from sewage when DO concentration decreased from 2.0 to 0.3mg/L, however, the nitrogen removal efficiency fluctuated greatly. The functional bacteria Proteobacteria remained dominant (~ 65%) under lower DO conditions, yet the total bacterial community structure changed in the aeration tank. The Nitrosomonas oligotropha-like ammonia-oxidizing bacteria, Nitrobacter winogradskyi spp., and Group1Nitrospira were identified as the major contributor of nitrification under low DO conditions. The quantitative PCR analysis showed that reducing DO concentration from 2.0 to 0.5mg/L enriched the nitrifiers (mainly Nitrospira), thereby ensuring complete nitrification under low DO conditions. The kinetics coefficient analysis also indicated that reducing DO concentration did not significantly affect the nitrifying bacterial growth, but retarded their decay to ensure biomass in the activated sludge system. These results provide positive support for wastewater treatment plants for saving energy by reducing DO concentration in the aeration tank.

Key words： low DO energy-saving nitrification A/O process microbial community plug-flow activated sludge system

收稿日期: 2019-12-06

PACS:

X703

基金资助:

中国人民大学科学研究基金项目（2018030194）

通讯作者: 王洪臣,教授,whc@ruc.edu.cn E-mail: whc@ruc.edu.cn

作者简介: 刘国华(1976-),内蒙古呼和浩特人,副教授,博士,主要研究方向为低碳污水处理技术、应用微生物技术.发表论文50余篇.

引用本文:

刘国华, 刘禹琛, 陈燕, 章乾, 徐相龙, 齐鲁, 王洪臣. 低DO对活性污泥系统碳和氮去除影响的研究[J]. 中国环境科学, 2020, 40(6): 2503-2512. LIU Guo-hua, LIU Yu-chen, CHEN Yan, ZHANG Qian, XU Xiang-long, QI Lu, WANG Hong-chen. Effects of low dissolved oxygen on carbon and nitrogen removal in activated sludge process. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2503-2512.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2020/V40/I6/2503

[1]	Mccarty P L, Bae J, Kim J.Domestic Wastewater Treatment as a Net Energy Producer-Can This be Achieved?[J].Environmental Science & Technology, 2011,45(17):7100-7106.
[2]	Reardon D J.Turning down the power [J].Civil Engineering, 1995, 65(8):54-56.
[3]	Stenstrom M K.Effects of oxygen transport limitation on nitrification in the activated sludge process [J].Reseach Journal WPCF, 1991,63(3): 208-219.
[4]	Metcalf & Eddy Inc.Wastewater Engineering: Treatment and Reuse [M].New York: McGraw-Hill press, 2003.
[5]	Park H D, Noguera D R.Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge [J].Water Research, 2004,38(14/15):0-3286.
[6]	Grady C P L, Daigger G T, Lim H C.Biological Wastewater Treatment [M].New York: CRC Press, 1999.
[7]	宗宫功,张荪楠,吴之丽.污水除磷脱氮技术[M].中国环境科学出版社, 1987. Zhong G, Zhang S, Wu Z.Technology of Phosphorus and Nitrogen Removal from Sewage [M].China Environmental Science Press, 1987.
[8]	王歆鹏,陈坚,华兆哲,等.硝化菌群在不同条件下的增殖速率和硝化活性[J].应用与环境生物学报, 1999,5(1):64-68. Wang X, Chen J, Hua Z, et al.The Optimum Growth and Nitrication Conditions of Nitrifying Bacteria [J].Chinese Journal of Applied & Environmental Biology.1999,5(1):64-68.
[9]	Stenstrom M K, Poduska R A.The effect of dissolved oxygen concentration on nitrification [J].Water Research, 1980,14(6):643-649.
[10]	Wagner M, Loy A, Nogueira R, et al.Microbial community composition and function in wastewater treatment plants [J].Antonie Van Leeuwenhoek, 2002,81(1-4):665-680.
[11]	Trillo I, Jenkins T E, Redmon D, et al.Implementation of Feedforward Aeration Control Using On-Line Offgas Analysis: The Grafton WWTP Experience [J].Proceedings of the Water Environment Federation, 2004,(7):27-45.
[12]	Abbassi B, Dullstein S, Räbiger N.Minimization of excess sludge production by increase of oxygen concentration in activated sludge flocs; experimental and theoretical approach [J].Water Research, 2000, 34(1):0-146.
[13]	Park H D, Noguera D R.Nitrospira community composition in nitrifying reactors operated with two different dissolved oxygen levels [J].Journal of microbiology and biotechnology, 2008,18(8):1470-1474.
[14]	Liu G, Wang J.Long-term low DO enriches and shifts nitrifier community in activated sludge [J].Environmental science & technology, 2013.
[15]	陈燕,刘国华,范强,等.不同溶解氧条件下A/O系统的除碳脱氮效果和细菌群落结构变化[J].环境科学, 2015,36(7):2610-2616. Chen Y, Liu G-h, Fan Q, et al.Carbon/nitrogen removal and bacterial community structure change in a A/O activated sludge system under different dissolved oxygen [J].Environmental Science, 2015,36(7): 2610-2616.
[16]	刘国华,陈燕,范强,等.溶解氧对活性污泥系统的脱氮效果和硝化细菌群落结构的影响[J].环境科学学报, 2016,36(6):1971-1978. Liu G-h, Chen Y, Fan Q, et al.Effects of dissolved oxygen concentration on nitrogen removal and nitrifying bacterial community structure in an activated sludge system [J].Acta Scientiae Circumstantiae, 2016,36(6):1971-1978.
[17]	国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社, 2006. State Environmental Protection Administration of China.Water and waste water monitoring and analysis method (Fourth Edition) [M].Beijing: China Environmental Science Press, 2006.
[18]	Zhou J.DNA recovery from soils of diverse composition [J].Appl.Environ.Microbiol, 1996,62(2):316-322.
[19]	Liu G H, Ye Z, Li H, et al.Biological treatment of hexanitrostilbene (HNS) produced wastewater using an anaerobic-aerobic immobilized microbial system [J].Chemical Engineering Journal, 2012,213(none): 118-124.
[20]	Zhi W, Ji G.Quantitative response relationships between nitrogen transformation rates and nitrogen functional genes in a tidal flow constructed wetland under C/N ratio constraints [J].Water Research, 2014,64(Complete):32-41.
[21]	Kim Y M, Cho H U, Lee D S, et al.Response of nitrifying bacterial communities to the increased thiocyanate concentration in pre-denitrification process [J].Bioresource Technology, 2011,102(2):913-922.
[22]	Hoshino T, Noda N, Tsuneda S, et al.Direct detection by in situ PCR of the amoA gene in biofilm resulting from a nitrogen removal process [J].Applied and Environmental Microbiology, 2001,67(11): 5261-5266.
[23]	Dionisi H M, Layton A C, Harms G, et al.Quantification of nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp.from full-scale wastewater treatment plants by competitive PCR [J].Applied and Environmental Microbiology, 2002,68(1):245-253.
[24]	Development and application of a PCR-denaturing gradient gel electrophoresis tool to study the diversity of Nitrobacter-like nxrA sequences in soil [J].FEMS Microbiology Ecology, 2008,63(2): 261-271.
[25]	Tamura K, Stecher G, Peterson D, et al.MEGA6: Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution, 2013,30(12):2725-2729.
[26]	Dong W Y, Gan G H, Wang H J, et al.Effect of DO on simultaneous removal of carbon and nitrogen by a membrane aerated bioreactor [J].Journal of Harbin Institute of Technology, 2011,43(2):50-55.
[27]	Guo J, Xu Y, Chen Y, et al.Long-term evolution of an activated sludge system under low dissolved oxygen conditions [J].Journal of Environmental Biology, 2013,34(2Spec No):427-436.
[28]	Hibiya K, Terada A, Tsuneda S, et al.Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane-aerated biofilm reactor [J].Journal of Biotechnology, 2003,100(1):23-32.
[29]	Bernet N, Dangcong P, Delgenès, Jean-Philippe, et al.Nitrification at Low Oxygen Concentration in Biofilm Reactor [J].Journal of Environmental Engineering, 2001,127(3):266-271.
[30]	Satoh H, Ono H, Rulin B, et al.Macroscale and microscale analyses of nitrification and denitrification in biofilms attached on membrane aerated biofilm reactors [J].Water Research, 2004,38(6):0-1641.
[31]	Semmens M J, Dahm K, Shanahan J, et al.COD and nitrogen removal by biofilms growing on gas permeable membranes [J].Water Research, 2003,37(18):4343-4350.
[32]	Semmens M J, Dahm K, Shanahan J, et al.COD and nitrogen removal by biofilms growing on gas permeable membranes [J].Water Research, 2003,37(18):4343-4350.
[33]	Dong W Y, Wang H J, Li W G, Ying W C, Gan G H, Yang Y, Effect of DO on simultaneous removal of carbon and nitrogen by a membrane aeration/filtration combined bioreactor, Journal of Membrane Science.2009,344:219-224.
[34]	Guo J S, Xu Y F, Chen Y P, Fang L F.Long-term evolution of an activated sludge system under low dissolved oxygen conditions, J.Environ.Biol.2013,34:427-436.
[35]	Laanbroek H J, Bodelier P L E, Gerards S.Oxygen consumption kinetics of Nitrosomonas europaeaand Nitrobacter hamburgensis grown in mixed continuous cultures at different oxygen concentrations [J].Archives of Microbiology, 1994,161(2):156-162.
[36]	Sliekers A O, Haaijer S C M, Stafsnes M H, et al.Competition and coexistence of aerobic ammonium-and nitrite-oxidizing bacteria at low oxygen concentrations [J].Applied Microbiology and Biotechnology, 2005,68(6):808-817.
[37]	Blackburne R, Vadiuelu V M, Yuan Z G, et al.Kinetic characterisation of an enriched Nitrospira culture with comparision to Nitrobacter [J].Water Resarch, 2007,41(14):3033-3042.
[38]	Almeida J S, Julio S M, Reis M et al.Nitrite inhibition of denitrification by Pseudomonas-Fluorescens [J].Biotechnology and Bioengineering, 1995,46(3):194-201.
[39]	Zhang T, Shao M F, Ye L.The ISME Journal - 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants [J].Isme Journal, 2012.
[40]	Wagner M R A, Amann R I, Lemmer H, et al.Probing activated sludge with oligonucleotides specific for Proteobacteria: Inadequacy of culture-dependent methods for describing microbial community Structure [J].Applied and Environmental Microbiology, 1993,59(5): 1520-1525.
[41]	Miura Y, Hiraiwa M N, Ito T, et al.Bacterial community structures in MBRs treating municipal wastewater: Relationship between community stability and reactor performance [J].Water Research, 2007,41(3):0-637.
[42]	Wang X, Wen X, Yu X, et al.Ammonia Oxidizing Bacteria Community Dynamics in a Pilot-Scale Wastewater Treatment Plant [J].PLoS ONE, 2012,7(4):e36272-.
[43]	Osborn A M, Moore E R B, Timmis K N.An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics [J].Environmental Microbiology, 2000,2.
[44]	Bie M J M D, Speksnijder A G C L, Kowalchuk G A, et al.Shifts in the dominant populations of ammonia-oxidizing β-subclass Proteobacteria along the eutrophic Schelde estuary Aquat [J].Aquatic Microbial Ecology, 2001,23(3):225-236.
[45]	Irvin S, Baker K, Li B.The variation of nitrifying bacterial population sizes in a sequencing batch reactor (SBR) treating low, mid, high concentrated synthetic wastewater [J].Journal of Environmental Engineering & Science, 2007,6(6):5008-5029.
[46]	Park H D, Noguera D R.Characterization of two ammonia-oxidizing bacteria isolated from reactors operated with low dissolved oxygen concentrations [J].Journal of applied microbiology, 2007,102(5): 1401-1417.
[47]	Koops H P, Purkhold U, Andreas Pommerening-Röser, et al.The lithoautotrophic ammonia-oxidizing bacteria [M].Springer New York, 2005.
[48]	Gieseke A, Purkhold U, Wagner M, et al.Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm, Applied and Environmental Microbiology, 2001,67:1351- 1362.
[49]	Fitzgerald, Colin M, Camejo, Oshlag J Z, et al.Ammonia-oxidizing microbial communities in reactors with efficient nitrification at low-dissolved oxygen [J].Water Research, 70:38-51.
[50]	Bernet N, Dangcong P, Delgenès, Jean-Philippe, et al.Nitrification at low oxygen concentration in biofilm reactor [J].Journal of Environmental Engineering, 2001,127(3):266-271.
[51]	Pickering R L.How hard is the biomass working?[D].UK: Newcastle University, Newcastle upon Tyne, 2008.
[52]	Dytczak M A, Londry K L, Oleszkiewicz J A.Activated sludge operational regime has significant impact on the type of nitrifying community and its nitrification rates [J].Water Research, 2008, 42(8/9):0-2328.
[53]	Limpiyakorn T, Shinohara Y, Kurisu F, et al.Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo [J].FEMS Microbiology Ecology, 2005, 54(2):205-217.
[54]	Hanaki K, Wantawin C, Ohgaki S.Nitrification at low levels of dissolved oxygen with and without organic loading in a suspended-growth reactor [J].Water Research, 1990,24(3):297-302.
[55]	Bellucci M, Ofiteru I D, Graham D W, et al.Low-dissolved-oxygen nitrifying systems exploit ammonia-oxidizing bacteria with unusually high yields [J].Applied and Environmental Microbiology, 2011,77(21): 7787-7796.