Abstract：In order to explore whether DEHP could damage cells through sirt1/pgc-1a pathway, HepG2 cells were cultured with different concentrations of DEHP at 1.6,8,40, 200,1000mmol/L for 24 or 48 hours. Cell viability was measured by CCK-8 assay. The intracellular ATP content and NO in the supernatants of cell cultures were measured by corresponding test kits. The level of TNF-a and IL-6 in cell supernatants were measured by enzyme-linked immunosorbent assay (ELISA), and the protein expression of sirt1, pgc-1a, nrf1, tfam were detected by Western blot. The results showed that: After treated with different concentrations of DEHP for 24 and 48 hours, the cell viability decreased significantly at high doses. DEHP could cause the decrease of ATP content and the increase of inflammatory factor content. No significant changes for the protein expression levels of sirt1, pgc-1a, nrf1, tfam had been found when exposed for 24 h. When exposed for 48 h, the protein content increased first and then decreased with the increase of dose, and sirt1 increased significantly at 8mmol/L. At the high dose of 1000mmol/L, all proteins decreased significantly. This study suggested that DEHP could induce the expression of sirt1/pgc-1a signal pathway to affect the mitochondrial biosynthesis and cause cell damage.
卜玲玲, 刘焕, 杨娇, 黄卓权, 靳登鹏, 柯倩华, 柳春红. DEHP经sirt1/pgc-1a通路诱导的HepG2细胞线粒体损伤效应[J]. 中国环境科学, 2020, 40(8): 3621-3626.
BU Ling-ling, LIU Huan, YANG Jiao, HUANG Zhuo-quan, JIN Deng-peng, KE Qian-hua, LIU Chun-hong. DEHP induced mitochondrial damage through sirt1/pgc-1a pathway in HepG2 cells. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(8): 3621-3626.
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