Transcriptome analysis in the liver of Nile tilapia (Oreochromis niloticus) after treated with di (2-ethylhexyl) phthalate
ZHANG Lin-bao, HU Ying, CHEN Hai-gang, JIA Xiao-ping, CAI Wei-gui
Scientific Observing and Experimental Station of South China Sea Fishery Resources & Environments, Ministry of Agriculture, Key Laboratory of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) and can induce diverse toxicities on aquatic organisms. To understand the transcriptomic responses of fish exposed to DEHP, we performed transcriptomic profiles in liver of tilapia (Oreochromis niloticus), which is the most important commercial fishes in Guangdong province. Transcriptome sequencing in liver of Nile tilapia exposed to olive oil (control group) and 50mg kg-1 body weight of DEHP for 7days was performed respectively using Illumina HiSeq 4000platform. A total of 30.10Mb and 30.16Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585unigenes. After comparing the two libraries, 3,217 and 1,791genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.
张林宝, 胡莹, 陈海刚, 贾晓平, 蔡文贵. 邻苯二甲酸二(2-乙基己基)酯对罗非鱼肝脏转录组影响研究[J]. 中国环境科学, 2019, 39(1): 386-396.
ZHANG Lin-bao, HU Ying, CHEN Hai-gang, JIA Xiao-ping, CAI Wei-gui. Transcriptome analysis in the liver of Nile tilapia (Oreochromis niloticus) after treated with di (2-ethylhexyl) phthalate. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(1): 386-396.
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