Effects of DEHP on hepatic tissue and cytochrome P450 enzymes in liver of rats
LIU Rui-jing1, HE Yong-jian1, LIU Huan1, ZHENG Dong-dong1, FAN Jia-sheng1, LIU Chun-hong1,2
1. Food College, South China Agricultural University, Guangzhou 510624, China;
2. Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China
To study the effects of di-(2-ethylhexyl) phthalate (DEHP) on hepatic tissue and cytochrome enzyme 450 in rat liver.A total of 32 male SD rats were randomly divided into 4groups (n=8per group):control group,low-dose group,medium-dose group,and high-dose group.DEHP was intragastrically administrated at the dosage of 0,300,1000 and 3000mg/kg (body weight) for 28days.The pathological changes of hepatic tissues were observed by HE staining.ELISA method was used to determine the content of CYP450,CYP2E1,CYP3A1 and PXR.Inflammatory cellular infiltration,hyperemia,vacuoles and fatty degeneration were observed in the low,middle,and high dosage groups respectively.The contents of CYP450 and CYP2E1 in the middle dosage group[(203.61±34.44) pmol/gprot,(14.57±3.03) U/gprot]and in the high dosage group[(263.73±63.78) pmol/gprot,(20.06±2.90) U/gprot]were significantly higher than that of the control group[(141.12±20.24) pmol/gprot,(10.76±2.24) U/gprot](P<0.01).CYP3A1 and PXR in all dosage groups increased significantly compared with the control group (P<0.05,P<0.01).DEHP exposure induced the levels of CYP450,CYP2E1,CYP3A1 and PXR in rat liver.
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LIU Rui-jing, HE Yong-jian, LIU Huan, ZHENG Dong-dong, FAN Jia-sheng, LIU Chun-hong. Effects of DEHP on hepatic tissue and cytochrome P450 enzymes in liver of rats. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(7): 2749-2754.
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