Effects of reductive soil disinfestation and biochar on soil enzyme activities and greenhouse gas emissions
JI Chun-yang1,2, HE Yun-hua3, SUN Xiao-fei1,2, MA Ya-pei1,2, MA Hong-liang1,2, GAO Ren1,2, YIN Yun-feng1,2
1. State Key Laboratory for Subtropical Mountain Ecology of the Ministry of Science and Technology and Fujian Province, Fujian Normal University, Fuzhou 350007, China; 2. School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, China; 3. Fuqing Modern Agriculture Development Center, Fuqing 350300, China
Abstract：In this study, four treatments, i.e., soil without any amendment as control (CK), soil amended with 2% (w/w) rice straw (RSD), soil amended with 2% (w/w) biochar (BC), and soil amended with 2% (w/w) rice straw and 2% (w/w) biochar (RSD+BC), were performed to compare their influences on soil enzyme activities and greenhouse gas emissions. The results showed that the activities of β-glucosidase (βG), cellobiohydrolase (CBH), peroxidase (PEO), β-1,4-N-acetylglucosaminidase (NAG), acid phosphatase (AP), and the values of enzyme C:P and N:P were significantly increased in RSD and RSD+BC treatments compared with CK (P<0.05), but there was no significant difference on enzyme activities in BC treatment. Soil CO2 emissions in RSD, BC, and RSD+BC treatments were increased by 10.6, 1.1, and 12.2times, respectively. Soil N2O emission in RSD treatment was also significantly increased, but significantly decreased in BC and RSD+BC treatments (P<0.05). Compared with RSD treatment, RSD+BC treatment notably decreased N2O emission and global warm potential (GWP) by 86.9% and 37.8%. Structural equation model analysis also indicated that βG and dissolved organic carbon (DOC) had a direct positive effect on soil CO2 emission, and βG and CBH indirectly influenced CO2 emission by affecting the DOC content. Moreover, soil NO3--N and NH4+-N had a direct negative effect on N2O emission. In summary, RSD+BC treatment was better in enhanced soil enzyme activities and decreased greenhouse gas emissions.
吉春阳, 何云华, 孙小飞, 马亚培, 马红亮, 高人, 尹云锋. 强还原与生物炭对土壤酶活性和温室气体排放的影响[J]. 中国环境科学, 2021, 41(2): 974-982.
JI Chun-yang, HE Yun-hua, SUN Xiao-fei, MA Ya-pei, MA Hong-liang, GAO Ren, YIN Yun-feng. Effects of reductive soil disinfestation and biochar on soil enzyme activities and greenhouse gas emissions. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(2): 974-982.
黄新琦,蔡祖聪.土壤微生物与作物土传病害控制[J]. 中国科学院院刊, 2017,32(6):593-600.Huang X Q, Cai Z C. Soil microbes and control of soil-borne diseases[J]. Bulletin of Chinese Academy of Sciences, 2017,32(6):593-600.
蔡祖聪.我国设施栽培养分管理中待解的科学和技术问题[J]. 土壤学报, 2019,56(1):36-43.Cai Z C. Scientific and technological issues of nutrient management under greenhouse cultivation in China[J]. Acta Pedologica Sinica, 2019,56(1):36-43.
蔡祖聪,张金波,黄新琦,等.强还原土壤灭菌防控作物土传病的应用研究[J]. 土壤学报, 2015,52(3):469-476.Cai Z C, Zhang J B, Huang X Q, et al. Application of redctive soil disinfestation to suppress soil-borne pathogens[J]. Acta Pedologica Sinica, 2015,52(3):469-476.
王宝英,李金泽,黄新琦,等.土壤强还原处理对连作芥蓝产量、微生物数量及活性的影响[J]. 土壤, 2019,51(2):316-323.Wang B Y, Li J Z, Huang X Q, et al. Effects of reductive soil disinfestation on yield, population and activity of microorganisms in continuously cropped soils of Chinese kale[J]. Soils, 2019,51(2):316-323.
Shennan C, Muramoto J, Koike S, et al. Anaerobic soil disinfestation is an alternative to soil fumigation for control of some soil-borne pathogens in strawberry production[J]. Plant Pathology, 2018,67(1):51-66.
朱文娟,王小国.强还原土壤灭菌研究进展[J]. 土壤, 2020,52(2):223-233.Zhu W J, Wang X G. Advances in method of reductive soil disinfestation[J]. Soils, 2020,52(2):223-233.
朱同彬,孟天竹,张金波,等.强还原方法对退化设施蔬菜地土壤的修复[J]. 应用生态学报, 2013,24(9):2619-2624.Zhu T B, Meng T Z, Zhang J B, et al. Effects of organic material amendment on vegetable soil nitrate content and nitrogenous gases emission under flooding condition[J]. Chinese Journal of Applied Ecology, 2013,24(9):2619-2624.
王小淇,索龙,季雅岚,等.添加几种秸秆并淹水对海南土壤N2O和CH4排放的影响[J]. 环境科学学报, 2017,37(10):4004-4010.Wang X Q, Suo L, Ji Y F, et al. Effect of addition of several types of straws on N2O and CH4emissions from soil under flooding condition in Hainan Province[J]. Acta Scientiae Circumstantiae, 2017,37(10):4004-4010.
Li B, Zhou J, Lu Y, et al. Field-aged biochar reduces the greenhouse gas balance in a degraded vegetable field treated by reductive soil disinfestation[J]. Environmental Science and Pollution Research, 2019,26(11):10609-10620.
孙小飞,吉春阳,杨柳明,等.生物质炭与强还原处理对退化设施蔬菜地土壤温室气体排放的影响[J]. 环境科学学报, 2020,40(4):1476-1482.Sun X F, Ji C Y, Yang L M, et al. Effects of biochar and reductive soil disinfestation on greenhouse gas emissions in degeaded facility vegetable soil[J]. Acta Scientiae Circumstantiae, 2020,40(4):1476-1482.
崔二苹,崔丙健,刘源,等.生物炭对非常规水源灌溉下土壤-作物病原菌的影响[J]. 中国环境科学, 2020,40(3):1203-1212.Cui E P, Cui B J, Liu Yuan, et al. Effects of biochar supplementation on the behavior of pathogens in soil-plant system under unconventional water resources irrigation[J]. China Environmental Science, 2020,40(3):1203-1212.
Jaiswal A K, Elad Y, Cytryn E, et al. Activating biochar by manipulating the bacterial and fungal microbiome through pre-conditioning[J]. New Phytologist, 2018,219(1):363-377.
Cayuela M L, van Zwieten L, Singh B P, et al. Biochar's role in mitigating soil nitrous oxide emissions:A review and meta-analysis[J]. Agriculture, Ecosystems & Environment, 2014,191(11):5-16.
Wang J, Zhong W H, Kang Y, et al. N2O emission mitigation and microbial activity after biochar and cao application in a flooded nitrate-rich vegetable soil[J]. Acta Agriculturae Scandinavica Section B-Soil and Plant Science, 2019,69(3):257-267.
Wu F P, Jia Z K, Wang S G. et al. Contrasting effects of wheat straw and its biochar on greenhouse gas emissions and enzyme activities in a Chernozemic soil[J]. Biology and Fertility of Soils, 2013,49:555-565.
Zi H B, Hu L, Wang C T, et al. Responses of soil bacterial community and enzyme activity to experimental warming of an alpine meadow[J]. European Journal of Soil Science, 2018,69(2):429-438.
Sinsabaugh R L, Lauber C L, Weintraub M N, et al. Stoichiometry of soil enzyme activity at global scale[J]. Ecology Letters, 2008,11(11):1252-1264.
王光飞,马艳,安霞,等.不同有机物料强还原处理对土壤性状影响与防控辣椒疫病效果[J]. 中国土壤与肥料, 2016,(5):24-129.Wang G F, Ma Y, An X, et al. Effect of different intensively reductive treatments by organic materials on soil character and disease control of phytophthora blight of chilli pepper[J]. Soil and Fertilizer Sciences in China, 2016,(5):24-129.
王光飞,马艳,郭德杰,等.不同用量秸秆生物炭对辣椒疫病防控效果及土壤性状的影响[J]. 土壤学报, 2017,54(1):204-215.Wang G F, Ma Y, Guo D J, et al. Application-rate-dependent effects of straw biochar on control of phytophthora blight of chilli pepper and soil properties[J]. Acta Pedologica Sinica, 2017,54(1):204-215.
周际海,袁东东,袁颖红,等.生物质炭与有机物料混施对土壤温室气体排放和微生物活性的影响[J]. 环境科学学报, 2018,38(7):2849-2857.Zhou J H, Yuan D D, Yuan Y H, et al. Effects of mixed application of biochar and organic fertilizers on soil greenhouse gases emission and microbial activity[J]. Acta Scientiae Circumstantiae, 2018,38(7):2849-2857.
Saiya-Cork K R, Sinsabaugh R L, Zak D R. The effects of long term nitrogen deposition on extracellular enzyme activity in an acer saccharum forest soil[J]. Soil Biology & Biochemistry, 2002,34(9):1309-1315.
王文锋,李春花,黄绍文,等.不同施肥模式对设施菜田土壤酶活性的影响[J]. 应用生态学报, 2016,27(3):873-882.Wang W F, Li C H, Huang S W, et al. Effects of different fertilization patterns on soil enzyme activities in greenhouse vegetable filed[J]. Chinese Journal of Applied Ecology, 2016,27(3):873-882.
Bonnett S A F, Ostle N, Freeman C. Seasonal variations in decomposition processes in a valley-bottom riparian peatland[J]. Science of the Total Environment, 2006,370(2):561-573.
Zhang L Y, Xiang Y Z, Jing Y M, et al. Biochar amendment effects on the activities of soil carbon, nitrogen, and phosphorus hydrolytic enzymes:A meta-analysis[J]. Environmental Science and Pollution Research, 2019,26(22):22990-23001.
张玉兰,陈利军,段争虎,等.荧光光谱法测定生物炭/秸秆输入土壤后酶活性的变化[J]. 光谱学与光谱分析, 2014,34(2):455-459.Zhang Y L, Chen L J, Duan Z H, et al. Change in soil enzymes activities after adding biochar or straw by fluorescent microplate method[J]. Spectroscopy and Spectral Analysis, 2014,34(2):455-459.
Elzobair K A, Stromberger M E, Ippolito J A. Stabilizing effect of biochar on soil extracellular enzymes after a denaturing stress[J]. Chemosphere, 2016,142:114-119.
Chen R R, Senbayram M, Blagodatsky S, et al. Soil C and N availability determine the priming effect:Microbial N mining and stoichiometric decomposition theories[J]. Global Change Biology, 2014,20(7):2356-2367.
仇存璞,陈晓芬,刘明,等.两种典型水稻土中秸秆碳转化的微生物过程[J]. 中国农业科学, 2019,52(13):2268-2279.Qiu C P, Chen X F, Liu M, et al. Microbial transformation process of straw-derived C in two typical paddy soils[J]. Scientia Agricultura Sinica, 2019,52(13):2268-2279.
Brassard P, Godbout S, Palacios J H, et al. Effect of six engineered biochars on GHG emissions from two agricultural soils:A short-term incubation study[J]. Geoderma, 2018,327(8):73-84.
Jones D L, Murphy D V, Khalid M, et al. Short-term biochar-induced increase in soil CO2release is both biotically and abiotically mediated[J]. Soil Biology & Biochemistry, 2011,43(8):1723-1731.
Luo Y, Durenkamp M, De Nobili M, et al. Short term soil priming effects and the mineralisation of biochar following its incorporation to soils of different pH[J]. Soil Biology & Biochemistry, 2011,43(11):2304-2314.
王军,施雨,李子媛,等.生物炭对退化蔬菜地土壤及其修复过程中N2O产排的影响[J]. 土壤学报, 2016,53(3):713-723.Wang J, Shi Y, Li Z Y, et al. Effects of biochar application on N2O emission in degraded vegetable soil and in remediatio process of the soil[J]. Acta Pedologica Sinica, 2016,53(3):713-723.
夏龙龙,颜晓元,蔡祖聪.我国农田土壤温室气体减排和有机碳固定的研究进展及展望[J]. 农业环境科学学报, 2020,39(4):834-841.Xia L L, Yan X Y, Cai Z C. Research progress and prospect of greenhouse gas mitigation and soil carbon sequestration in croplands of China[J]. Journal of Agro-Environment Science, 2020,39(4):834-841.
郎漫,李平,李淼,等.浅层淹水条件下不同施肥处理对黑土温室气体排放的影响[J]. 中国环境科学, 2019,39(6):2506-2513.Lang M, Li P, Li M, et al. Effects of different fertilization treatments on greenhouse gas emissions from black soil under shallow flooding condition[J]. China Environmental Science, 2019,39(6):2506-2513.
雷宏军,刘欢,臧明,等.曝气灌溉条件下土壤N2O排放特征及影响因子分析[J]. 中国环境科学, 2019,39(5):2115-2122.Lei H J, Liu H, Zang M, et al. Characteristics and influencing facters of N2O emission from incubated soil under aerated irrigation[J]. China Environmental Science, 2019,39(5):2115-2122.
杜莎莎,王朝旭.氨氧化过程中稻壳生物炭抑制酸性农田土壤N2O排放[J]. 中国环境科学, 2020,40(1):85-91.Du S S, Wang C X. Rice husk-derived biochar inhibited N2O emission from acidic arable soil under conditon of facilitating ammonia oxidation[J]. China Environmental Science, 2020,40(1):85-91.
Harter J, Krause H M, Schuettler S, et al. Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community[J]. The ISME Journal, 2014,8(3):660-674.
Van Zwieten L, Singh B P, Kimber S W L, et al. An incubation study investigating the mechanisms that impact N2O flux from soil following biochar application[J]. Agriculture Ecosystems & Environment, 2014, 191(11):53-62.
Nelissen V, Saha B K, Ruysschaert G, et al. Effect of different biochar and fertilizer types on N2O and NO emissions[J]. Soil Biology & Biochemistry, 2014,70(3):244-255.
Harter J, Weigold P, El-Hadidi M, et al. Soil biochar amendment shapes the composition of N2O-reducing microbial communities[J]. Science of the Total Environment, 2016,562(15):379-390.