Effect of dissolved oxygen on the community and activities of functional bacteria in anammox biofilter
WU Wen-jun, LIU Xiu-hong, CUI Bin, LIU Run-yu, YANG Qing
Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
Abstract:The current researches on microorganisms in anammox system were mainly focused on anaerobic ammonium oxidation bacteria. The microbial community structure and the key kinetic parameters of anaerobic ammonium oxidation bacteria (AnAOB), ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and denitrifying bacteria (DNB) in anammox filter were measured and analyzed in this study. Moreover, the effects of DO concentration in the range of 0.2~1.5mg/L on the activity of AnAOB, AOB and NOB were investigated. The results showed that the anammox filter of long-term stable operation was still a mixed system with multiple bacterial groups, in which anammox bacteria was the main function bacteria. The activity of AnAOB with 5.3mgN/(gVSS·h) was the highest among the functional bacteria in the filter; whereas DNB and AOB had a certain activity. When DO concentration increased from 0.2mg/L to 1.5mg/L, the activity of AnAOB almost kept stable; whereas, the specific ammonia oxidation rate of AOB increased from 0.76mgN/(gVSS·h) to 1.08mgN/(gVSS·h). The obtained oxygen half saturation constant (KO2, AOB) of AOB was (0.106 ±0.010) mg/L, which indica ted that AOB has high affinity for oxygen. During the entire experiment, the activity of NOB was not detected. Therefore, in the anammox system, the main functional bacteria coexisted and competed each other for substrates.
武文君, 刘秀红, 崔斌, 刘润雨, 杨庆. 溶解氧对Anammox滤池内功能菌群及活性的影响[J]. 中国环境科学, 2021, 41(3): 1415-1421.
WU Wen-jun, LIU Xiu-hong, CUI Bin, LIU Run-yu, YANG Qing. Effect of dissolved oxygen on the community and activities of functional bacteria in anammox biofilter. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(3): 1415-1421.
Jetten M S M, Wagner M, Fuerst J, et al. Microbiology and application of the anaerobic ammonium oxidation (‘ANAMMOX’) process[J]. Current Opinion in Biotechnology, 2001,12(3):283-288.
[2]
Zekker I, Aimar Kivirüüt, Rikmann E, et al. Enhanced Efficiency of Nitritating-Anammox Sequencing Batch Reactor Achieved at Low Decrease Rates of Oxidation-Reduction Potential[J]. Environmental Engineering ence, 2019,36(3):350-360.
[3]
Tim L.G. Hendrickx,Yang W, et al.Autotrophic nitrogen removal from low strength waste water at low temperature[J]. Water Research, 2012,46(7).
[4]
Meng Y B, Zhou Z B, Meng F G. Impacts of diel temperature variations on nitrogen removal and metacommunity of anammox biofilm reactors[J]. Water Research, 2019,160.
[5]
Kuenen J G. Anammox bacteria:from discovery to application[J]. Nature Reviews Microbiology, 2008,6(4):320-326.
[6]
Jin R C, Yang G F, Yu J J, et al. The inhibition of the Anammox process:A review[J]. Chemical Engineering Journal, 2012,197:67-79.
[7]
Meng Y, Zhou Z, Meng F. Impacts of diel temperature variations on nitrogen removal and metacommunity of anammox biofilm reactors[J]. Water Research, 2019,160:1-9.
[8]
秦宇,方芳,郭劲松,等.溶解氧对单级自养脱氮系统功能菌数量的影响[J]. 微生物学报, 2009,49(6):773-779. Qin Y, Fang F, Guo J S, et al. Quantification of functional bacteria in one-step completely nitrogen removal process influenced by dissolved oxygen[J]. Acta Microbiologica Sinica, 2009,49(6):773-779.
[9]
Cao Y S, Kwok B H, Mark C M, et al. The influence of dissolved oxygen on partial nitritation/anammox performance and microbial community of the 200,000m3/d activated sludge process at the Changi water reclamation plant (2011 to 2016)[J]. Water science and technology:a journal of the International Association on Water Pollution Research, 2018,78(3).
[10]
Zhu W Q, Zhang P Y, Yu D S, et al. Nitrogen removal performance of anaerobic ammonia oxidation (ANAMMOX) in presence of organic matter[J]. Biodegradation, 2017,28(2/3):159-170.
[11]
时兴东.养殖废水中重金属及抗生素对厌氧氨氧化工艺的影响研究[D]. 重庆:重庆大学, 2019. Shi X D. Effects of heavy metals and antibiotics in aquaculture wastewater on Anammox process[D]. Chongqing:Chongqing University, 2019.
[12]
国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2002.
[13]
Zhou X, Liu X, Huang S, et al. Total inorganic nitrogen removal during the partial/complete nitrification for treating domestic wastewater:Removal pathways and main influencing factors[J]. Bioresource Technology, 2018,256:285-294.
[14]
周薛扬.快速实现生活污水低氧深度脱氮的方法及氮转化途径研究[D]. 北京:北京工业大学, 2018. Zhou X Y. Research on fast realization methods of deep nitrogen removal and nitrogen conversion pathways from domestic sewage[D]. Beijing:Beijing University Of Technology, 2018.
[15]
Li B, Wang Y, Li X, et al. Comparing the nitrogen removal performance and microbial communities of flocs-granules hybrid and granule-based CANON systems[J]. Science of The Total Environment, 2020,703:134949.
[16]
Cui B, Yang Q, Zhang Y, et al. Improving nitrogen removal in biological aeration filter for domestic sewage treatment via adjusting microbial community structure[J]. Bioresource Technology, 2019, 293:122006.
[17]
Liu Y, Niu Q G, Wang S P, et al. Upgrading of the symbiosis of Nitrosomanas and anammox bacteria in a novel single-stage partial nitritation-anammox system:Nitrogen removal potential and Microbial characterization[J]. Bioresource Technology, 2017,244:463-472.
[18]
Kindaichi T, Yuri S, Ozaki N, et al. Ecophysiological role and function of uncultured Chloroflexi in an anammox reactor[J]. Water science and technology:a journal of the International Association on Water Pollution Research, 2012,66(12):2556-2561
[19]
杨忠启,刘秀红,李海鑫,等.上向流厌氧滤池(UAF)处理城市生活污水的运行效能[J]. 环境科学, 2019,40(9):4121-4127. Yang Z Q, Liu X H, Li H X, et al. Performances analysis of an upflow anaerobic filter for domestic sewage treatment[J]. Environmental Science, 2019,40(9):4121-4127.
[20]
Hao X, Joseph J, Heijnen, et al. Sensitivity analysis of a biofilm model describing a one-stage completely autotrophic nitrogen removal (CANON) process[J]. Biotechnology & Bioengineering, 2002,77(3):267-277.
[21]
Urbain V, Mobarry B, de Silva V, et al. Integration of performance, molecular biology and modeling to describe the activated sludge process[J]. Water Science and Technology, 1998,37(4/5):223-229.
[22]
陈宏宇.膜生物反应器中SMP的特性及其对膜污染影响的研究[D]. 天津:天津大学, 2007. Chen H Y. Research on Characteristic of SMP and it's Influence on Membrane Fouling in Membrane Biological Reactor[D]. Tianjin:Tianjin University, 2007.
[23]
李健敏.两种典型工艺中的氮素转化途径研究[D]. 北京:北京工业大学, 2019. Li J M. Analysis of Nitrogen Conversion Pathways in Two Typical Processes[D]. Beijing:Beijing University Of Technology, 2019.
[24]
李亚峰,张文静,马晨曦.pH值和DO对厌氧氨氧化脱氮性能的影响[J]. 沈阳建筑大学学报(自然科学版), 2013,29(4):715-720. Li Y F, Zhang W J, Ma C X. The impact of pH and DO on nitrogen removal of anammox[J]. Journal of Shenyang Jianzhu University (Natural Science), 2013,29(4):715-720.
[25]
Fan H, Qi L, Liu G, et al. Aeration optimization through operation at low dissolved oxygen concentrations:Evaluation of oxygen mass transfer dynamics in different activated sludge systems[J]. Journal of Environmental Sciences, 2017,55(5):224-235.
[26]
Zhang M, Yu M, Wang Y X, et al. Operational optimization of a three-stage nitrification moving bed biofilm reactor (NMBBR) by obtaining enriched nitrifying bacteria:Nitrifying performance, microbial community, and kinetic parameters[J]. The Science of the total environment, 2019,697:134101.
[27]
Manser R, Gujer W, Siegrist H. Consequences of mass transfer effects on the kinetics of nitrifiers[J]. Water Research, 2005,39(19):4633-4642.
[28]
杨庆,杨玉兵,刘秀红,等.不同溶解氧环境下氨氧化菌的氧半饱和常数比较[J]. 中国给水排水, 2017,33(23):22-26. Yang Q, Yang Y B, Liu X H, et al. Oxygen half-saturation constants of ammonia oxidizing bacteria (AOB) in nitrification sludge under different dissolved oxygen[J]. China Water & Wastewater, 2017, 33(23):22-26.
[29]
Gürkan Sin, Kaelin D, Kampschreur M J, et al. Modelling nitrite in wastewater treatment systems:A discussion of different modelling concepts[J]. Water Science & Technology, 2008,58(6):1155-1171.
[30]
Wu P, Zhang X X, Wang X Z, et al. Characterization of the start-up of single and two-stage Anammox processes with real low-strength wastewater treatment. 2020,245:125572.
[31]
杨玉兵.两段式短程硝化-厌氧氨氧化脱氮性能及其N2O产生途径研究[D]. 北京:北京工业大学, 2018. Yang Y B. Two-stage partial nltrification-anaerobic ammonium oxidation denitrification performance and its N2O production pathway[D]. Beijing:Beijing University Of Technology, 2018.
[32]
贾方旭,彭永臻,杨庆.厌氧氨氧化菌与其他细菌之间的协同竞争关系[J]. 环境科学学报, 2014,34(6):1351-1361. Jia F X, Peng Y Z, Yang Q. Competition and synergism between anammox bacteria and other bacteria[J]. Acta Scientiae Circumstantiae, 2014,34(6):1351-1361.