基于氨氮,硝氮及乙酸条件下Anammox菌的培养

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摘要为探讨Anammox菌在氨氮、硝氮及乙酸条件下的富集特性，采用某城市污水处理厂A²/O系统中的生物填料作为MBBR的载体直接启动并运行.结果表明，在NH₄⁺-N、NO₃^--N及乙酸为基质的培养条件下，Anammox菌可在部分反硝化和厌氧氨氧化协同作用下快速富集.经过130d的富集培养，MBBR处理负荷（以N计）达到920.79mg/（m²·d），Anammox活性（以NH₄⁺-N计）达到3 018.19mg/（m²·d）.高通量结果显示，经富集培养后，Ca.Brocadia占比从0.89%增至27.80%，为Anammox菌的主导菌属；Thauera占比从0.01%增至6.75%，Flavobacterium占比从0.29%增至11.72%，为部分反硝化菌的主导菌属.

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关键词 ：厌氧氨氧化, 富集培养, 移动床生物膜反应器, 生物脱氮

Abstract：In order to investigate the characteristic of anammox enrichment process with ammonium, nitrate and acetic acid as substrates, an MBBR with mediums from A²/O system treating municipal wastewater was established to start up anammox process. The results showed that the enrichment of anammox bacteria, with ammonium, nitrate and acetic acid as substrates, combined with denitrification process could be obtained rapidly. TN removal load reached to 920.79mg/(m²·d), and Anammox activity could attain to 3018.19mg/(m²·d) after 130d enrichment. Quantitative PCR results showed that after enrichment, the proportion of Ca. Brocadia increased from 0.89% to 27.80%, which is the dominated community of anammox bacteria. The proportion of Thauera increased from 0.01% to 6.75%, and the proportion of Flavobacterium increased from 0.29% to 11.72%, which are the dominated communities of partial denitrifying bacteria.

Key words： anaerobic ammonium oxidation enrichment moving-bed biofilm reactor biological nitrogen removal

收稿日期: 2020-03-10

PACS:

X703.5

基金资助:陕西省2019年重点研发计划（2019ZDLSF06-05）；陕西省2020年重点研发计划（2020SF-357）

通讯作者: 彭党聪,教授,dcpeng@xauat.edu.cn E-mail: dcpeng@xauat.edu.cn

作者简介: 吕恺(1993-),男,甘肃平凉人,西安建筑科技大学博士研究生,主要从事污水生物处理.发表论文1篇.

引用本文:

吕恺, 王康舟, 姚雪薇, 赵文钊, 彭党聪, 李惠娟, 韩芸. 基于氨氮,硝氮及乙酸条件下Anammox菌的培养[J]. 中国环境科学, 2020, 40(10): 4348-4353. Lü, Kai, WANG Kang-zhou, YAO Xue-wei, ZHAO Wen-zhao, PENG Dang-cong, LI Hui-juan, HAN Yun. Enrichment of Anammox under conditions of ammonium, nitrate and acetic acid as substrates. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(10): 4348-4353.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2020/V40/I10/4348

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