Biodegradation of 3,5,6-trichloro-2-pyridinol by Cupriavidus sp. DT-1 in liquid and soil environments
LU Peng, ZHOU Hui, YUAN Meng
Anhui Key Laboratory of Molecular Enzymology and Major Disease Mechanism research, College of Life Sciences, Anhui Normal University, Wuhu 241000, China
Abstract:Cupriavidus sp. DT-1 was a 3,5,6-trichloro-2-pyridinol (TCP)-degrading strain which could transform TCP to 2-hydroxypyridine (2-HP). Liquid-mass spectrometry (HPLC-MS) was used to detect the degradation products of 2-HP, And the methods of triparetal conjugation, quantitative real-time PCR (q-PCR) were used to evaluate the remediation effect of TCP-contaminated soils by the degrading-bacterium. Results showed that strain DT-1 was able to further degrade 2-HP, and sequentially produced nicotine blue, maleamic acid and fumaric acid, until it was transformed into the carbon source that could support the growth of strain DT-1. Pilot experiment showed that inoculation of strain DT-1 remarkably accelerated the elimination of TCP in soils. The degradation rates of TCP in inoculated soils were 94.4% and 86.7%, while those in uninoculated soils were 20.4% and 28.4%, respectively. Green fluorescent protein encoding gene gfp harbored strain DT-1-gfp could survive in soils for more than 35d. The results of q-PCR showed that inoculation of strain DT-1-gfp significantly improved the recovery of bacterial community abundance in the TCP-contaminated soils.
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