Abstract：In order to solve the problems of particle disintegration and microbial inactivation during long-term storage of aerobic granular sludge, phenol solutions at concentrations of 30, 60, 100, 150, 200 and 300mg/L were chosen to store aerobic granular sludge, and distilled water was used as control group for 150d at room temperature. The changes of aerobic sludge characteristics in each storage solution during storage were investigated. The results showed that aerobic granular sludge in 60mg/L phenol solution had better particle structure and compactness, and aerobic granular sludge released less contaminant into storage solution after 150d of storage. Aerobic particulate sludge stored in 200 and 300mg/L phenol solutions also had some structural integrity, but the particulate structure strength was poor. The abundance of dominant bacterial orders (Flavobacteria, Proteobacteria, and Actinobacteria) in aerobic granular sludge decreased and the abundance of Clostridia increased. The concentration of c-di-GMP in 60mg/L phenol solution was 14.38μg/gMLSS, it decreased of 36.68% compared to the pre-storage level. The concentration of c-di-GMP in 300mg/L phenol solution was 1.24μg/gMLSS, it decreased of 94.54%. The 60mg/L phenol solution facilitated the secretion of c-di-GMP signaling molecules by the Flavobacteria in aerobic granular sludge, increasing the storage stability of the granular sludge. The short-term (1~70d) and long-term (70~150d) aerobic granular sludge were recommended to be stored in 30 and 60mg/L phenol solution respectively, which was beneficial to maintain the microbial activity of granular sludge.
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LIU Qian-jin, LIU Li-fan. Effect of phenol solution on storage stability of aerobic granular sludge. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5620-5626.
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