Effect of low-dose ultraviolet-A (UVA) irradiation on nitrification activity of activated sludge and oxidative stress response
HUANG Xiao-yu1, CHU Zhao-rui1,2, RONG Hong-wei1,2, HE Jian-feng1, HUANG Dan-dan1, WANG Zhi-hui1, LAI Guo-wang1
1. School of Civil Engineering, Guangzhou University, Guangzhou 510006, China; 2. Guangdong Engineering Research Center of Water Quality Safety and Pollution Control, Guangzhou 510006, China
Abstract:The effect of illumination intensity (0, 0.15, 0.39, 0.62 and 1.16μE/(L·s)) and duration (0, 1, 2, 3 and 4h) of UVA on the activities of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were investigated in well-performed nitrifying activated sludge. The results showed UVA irradiation significantly reduced activity of NOB (P < 0.01), while effect of UVA on AOB was insignificant (P > 0.05). AOB activity and NOB activity showed more significant difference as UVA irradiation prolonged. Dynamics modelling indicated that the decay rate of NOB (b=0.6938h-1) was much higher than that of AOB (b=0.1423h-1) under UVA, which implied that NOB was rather intolerant to UVA compared with AOB. The difference between AOB and NOB activities under UVA irradiation may associated with the oxidative stress effect induced by UVA, which would dramatically increase the level of intracellular reactive oxygen species (ROS), and thus damage the cell membrane, destroy the structural integrity of cell, and cause the cell decay.
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