The stability of microbiological indicators in aerobic and anaerobic environment
ZHANG Yang1, ZHENG Qian-xing1,2, WU Ren-ren1, LI Guo-dong1, ZHONG Yi1, CHEN Zhong-ying1, WEI Si-ye1, LI Kai-ming1
1. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655, China; 2. Faculty of Environment Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China
Abstract：We investigated the variations of pig-associated genetic markers, several microbiological indicators and potential pathogens in anaerobic and aerobic treatments using qPCR and Illumina sequencing methods. The six indicators (Streptococcaceae、Lactobacillaceae、Carnobacteriaceae、Ruminococcaceae、Lachnospiraceae and Prevotellaceae) had a similar decay trend in anaerobic and aerobic treatments, but these taxa persisted longer in anaerobic than aerobic treatments. Hence, the six indicators all can be used for identifying the recent pollution issues in aerobic water environment. The genetic markers including GenBac and Pig-2-Bac showed biphasic decay model in both aerobic and anaerobic treatments, and the first decay stage contributed the majority decay. However, the decay rates of genetic markers were significantly higher in aerobic than anaerobic treatments, which suggests the poor stability of the markers in aerobic water environment. For the identified potential pathogens, the Bacteroidales genetic markers presented higher correlation with Prevotella, Pseudomonas and Erysipelothrix(P<0.05), but only Prevotella showed similar correlation coefficient in both anaerobic and aerobic treatments (0.913~0.953), which demonstrate the usefulness of the Bacteroidales genetic markers for predicting the presence of Prevotella.
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