The preparation of Acidithiobacillus thiooxidans immobilized pellet and its coupling removal of H2S with packing material
SHI Chao-hong1, LU Yu-sheng1, GU Wen-jie1,2, XIE Kai-zhi1, XU Pei-zhi1, WANG Dan1, PENG Huan-long1, LI Ya-ying1, SUN Li-li1
1. Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture, Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation, Guangdong Engineering Research Center of Soil Microbes and Cultivated Land Conservation, Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China
Abstract：Acidithiobacillus thiooxidans was immobilized to prepare bacterial pellet using sodium alginate (SA) as the carrier. The microbial immobilization process was optimized and the removal capacity of the bacterial pellet to H2S was examined in a biofilter. Meanwhile, acclimated activated sludge containing sulfur malodorous eliminating microbial consortium obtained previously was employed as bacterial source to screen packing media in biofilter. Lastly, A. thiooxidans immobilized pellet and the screened packing media were coupled to biotrickling filtration deodorization. The results showed that the optimal conditions for microbial immobilization process were 3.0% of SA, adding adsorbent carbon nanotubes (CNT) into SA solution, keeping the ratio of A. thiooxidans bacterial suspension solution to the mixture solution at 20%, 4.0% of CaCl2, suspending immobilized pellet into hexanediamine (HDA) solution for surface modification. Filling A. thiooxidans immobilized pellet into biofilter to eliminate H2S, and the removal efficiency and elimination capacity of H2S reached 70% and 1.06g H2S/m3·h. Activated carbon cloth was screened as the best packing media among polyurethane foam, activated carbon cloth and ceramsite, and the removal efficiency and elimination capacity of H2S reached 88% and 0.84g H2S/m3·h in this case. Filling A. thiooxidans immobilized pellet and activated carbon cloth into biofilter with mixing pattern to eliminate H2S, the removal efficiency and elimination capacity of H2S reached 86% and 1.00g H2S/m3·h.
石超宏, 卢钰升, 顾文杰, 解开治, 徐培智, 王丹, 彭焕龙, 李雅莹, 孙丽丽. 氧化硫硫杆菌固定化菌球制备及其耦合填料去除H2S[J]. 中国环境科学, 2022, 42(5): 2331-2338.
SHI Chao-hong, LU Yu-sheng, GU Wen-jie, XIE Kai-zhi, XU Pei-zhi, WANG Dan, PENG Huan-long, LI Ya-ying, SUN Li-li. The preparation of Acidithiobacillus thiooxidans immobilized pellet and its coupling removal of H2S with packing material. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2331-2338.
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