Lead-induced alterations in hepatocyte biomolecules based on Raman spectroscopy
XING Yu1,2, PANG Wei-yi2, XU Li1
1. Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2. School of Public Health, Guilin Medical University, Guilin 541199, China
Abstract：In this study, we employed Raman spectroscopy to explore the bio-molecular alterations in HepG2cells following exposure to lead with concentrations of 0.05, 0.1, 0.5, 1, 5mg/L, which took different time periods (0.5, 1.5, 3, 6, 12, 24h) respectively. Raman spectra correlated to cellular biological information was collected via Raman microscopy to further investigate the bio-molecular alterations of liver cells community following lead exposure. It showed that although the spectra in each treatment group were similar, certain diversities of the peaks positions and the absorption intensities were observed. Additionally, multivariate analysis was applied to the spectral dataset to determine the biological changes underlying the exposure, and it showed that the spectral data of cellular groups following exposures at different concentrations with the same exposure time showed a discrete trend on LD1, and it was significantly observed in 24-h exposure. The results, as well, indicated that lead exposure can damage the structure of liver cell proteins, lipids, nucleic acids, carotenoids, carbohydrates and affect their normal functions. These cellular responses are dose-related and increases with exposure time. In conclusion, our study suggests that Raman spectroscopy is proper tool with ability to detect the biological molecular changes in liver cells following lead exposure. It can not only provide a new assay in toxicology research, but also provides a theoretical basis for rapid safety evaluation of environmental pollutants.
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