Start-up and performance study on the simultaneous nitrification-endogenous denitrification phosphorus removal (SNEDPR) in the biological process of the moving bed biofilm reactor (MBBR)
JING Shuang-yi, SONG Zi-yang, LIU Chao, LI Wei-ping, LI Qi, ZHANG Tie-jun
School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Abstract:This study assessed the feasibility of simultaneous nitrification, endogenous denitrification and phosphorus removal (SNEDPR) for enhancing the nitrogen and phosphorus removal by the process of moving bed biofilm reactor (MBBR), an MBBR operated alternately by continuous aeration and stirring/aeration was adopted for simulating the treatment of domestic sewage with magnetic filler as the carrier. The performance of nitrogen and phosphorus removal during SNEDPR initiation was investigated, and the structural changes of functional microflorae were analyzed by fluorescence microscopy and high-throughput sequencing technology. The results showed that after two stages of operation, the removal rates of ammonia nitrogen and phosphorus reached 97.6% and 85.37% respectively, and the NO2--N, NO3--N and COD concentrations in the effluent were 1.3949, 3.88 and 20.4mg/L respectively. The simultaneous nitrification and endogenous denitrification rate (SNEDR) increased from 0.07% to 86.35%. The improvement in SNEDR at the aerobic stage decreased the NOx--N concentration in the effluent, improved the denitrification performance of the system and promoted the storage capacity of carbon source at the anaerobic stage. The fluorescence microscopy and high-throughput sequencing results showed that after 53d of operation, the diversity of microbial community was improved significantly, and at the same time, the increase in abundance for GAOs, AOB and NOB (from 3.3%, 0.84% and 0.66% in the inoculated sludge to 27.08%/20.48%, 1.45%/1.76% and 1.05%/0.85% in the system respectively) and the presence of PAOs and DPAOs guaranteed the performance of nitrogen and phosphorus removal for the system, and realized the coupling of EBPR and SNED by the MBBR process.
敬双怡, 宋子洋, 刘超, 李卫平, 李奇, 张铁军. MBBR工艺中SNEDPR的启动及性能研究[J]. 中国环境科学, 2022, 42(7): 3121-3129.
JING Shuang-yi, SONG Zi-yang, LIU Chao, LI Wei-ping, LI Qi, ZHANG Tie-jun. Start-up and performance study on the simultaneous nitrification-endogenous denitrification phosphorus removal (SNEDPR) in the biological process of the moving bed biofilm reactor (MBBR). CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(7): 3121-3129.
Zhang W T, Peng Y Z, Ren N Q, et al. Improvement of nutrient removal by optimizing the volume ratio of anoxic to aerobic zone in AAO-BAF system[J]. Chemosphere, 2013,93(11):2859-2863.
[2]
赵伟华,王梅香,李健伟,等.A2O工艺和A2O+BCO工艺的脱氮除磷性能比较[J].中国环境科学, 2019,39(3):994-999. Zhao W H, Wang M X, Li J W, et al. Comparison of nitrogen and phosphorus removal performance between A2O process and A2O+BCO process[J]. China Environmental Science, 2019,39(3):994-999.
[3]
黄健平,闫阁,卞晓峥,等.反硝化除磷污水处理工艺影响因素分析[J].华北水利水电大学学报(自然科学版), 2021,42(6):100-106. Huang J P, Yan G, Bian X Z, et al. Analysis on influencing factors of denitrifying phosphorus removal wastewater treatment process[J]. Journal of North China University of Water Resources and Hydropower (Natural Science Edition), 2021,42(6):100-106.
[4]
李洪静,陈银广,顾国维.丙酸的加入对厌氧-低氧同时生物除磷脱氮系统的影响[J].环境科学, 2007,(8):1681-1686. Li H J, Chen Y G, Gu G W. Effect of propionic acid on anaerobic hypoxia simultaneous biological phosphorus and nitrogen removal system[J]. Environmental Science, 2007,(8):1681-1686.
[5]
黄崇,袁林江,牛晚霞,等.投加填料对微生物群落结构的影响及对水质的变化研究[J].中国环境科学, 2021,41(1):207-213. Huang C, Yuan L J, Niu W X, et al. Study on the effect of adding fillers on microbial community structure and water quality[J]. China Environmental Science, 2021,41(1):207-213.
[6]
向元婧,蒋潇.MBBR工艺在市政污水处理中的应用及展望[J].绿色科技, 2021,23(2):84-86. Xaing Y J, Jiang X. Application and prospect of MBBR process in municipal sewage treatment[J]. Green Technology, 2021,23(2):84-86.
[7]
史晓林.固体碳源对MBBR短程硝化反硝化脱氮性能的影响研究[D].合肥:安徽大学, 2019. Shi X L. Effect of solid carbon source on the performance of MBBR shortcut nitrification and denitrification[D]. Hefei:Anhui University, 2019.
[8]
Xu X Y, Liu G, Zhu L. Enhanced denitrifying phosphorous removal in a novel anaerobic/aerobic/anoxic (AOA) process with the diversion of internal carbon source[J]. Bioresource Technology, 2011,102(22):10340-10345.
[9]
Kuba T, Van Loosdrecht M C, Heijnen J J. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system[J]. Water Research, 1996,30(7):1702-1710.
[10]
Jabari P, Munz G, Oleszkiewicz J A. Selection of denitrifying phosphorous accumulating organisms in IFAS systems:comparison of nitrite with nitrate as an electron acceptor[J]. Chemosphere, 2014, 109:20-27.
[11]
Zeng R J, Lemaire R, Yuan Z, et al. Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor[J]. Biotechnology and Bioengineering, 2003,84(2):170-178.
[12]
王晓霞,王淑莹,赵骥,等.厌氧/好氧SNEDPR系统处理低C/N污水的优化运行[J].中国环境科学, 2016,36(9):2672-2680. Wang X X, Wang S Y, Zhao J, et al. Optimal operation of anaerobic/aerobic SNEDPR system for low C/N wastewater treatment[J]. China Environmental Science, 2016,36(9):2672-2680.
[13]
方茜,张朝升,张立秋,等.同时硝化/反硝化除磷工艺稳定性控制研究[J].给水排水, 2014,50(11):132-135. Fang Q, Zhang Z S, Zhang L Q, et al. Study on stability control of simultaneous nitrification/denitrification phosphorus removal process[J]. Water Supply and Drainage, 2014,50(11):132-135.
[14]
甄建园.低C/N城市污水同步硝化内源反硝化脱氮除磷性能及优化研究[D].青岛:青岛大学, 2019. Zhen J Y. Performance and optimization of simultaneous nitrification and endogenous denitrification for nitrogen and phosphorus removal in low C/N municipal wastewater[D]. Qingdao:Qingdao University, 2019.
[15]
甄建园,于德爽,王晓霞,等.低C/N (
[16]
Oehmen A, Saunders A M, Vives M T, et al. Competition between polyphosphate and glycogen accumulating organisms in enhanced biological phosphorus removal systems with acetate and propionate as carbon sources[J]. Journal of Biotechnology, 2006,123(1):22-32.
[17]
Ge S J, Peng Y Z, Lu C C, et al. Practical consideration for esign and optimization of the step feed process[J]. Frontiers of Environmental Science&Engineering, 2012,7(1):135-142.
[18]
李冬,李悦,李雨朦,等.好氧颗粒污泥同步硝化内源反硝化脱氮除磷[J].中国环境科学, 2022,42(3):1113-1119. Li D, Li Y, Li Y M, et al. Simultaneous nitrification of aerobic granular sludge and endogenous denitrification for nitrogen and phosphorus removal[J]. China Environmental Science, 2022,42(3):1113-1119.
[19]
Coma M, Verawaty M, Pijuan M, et al. Enhancing aerobic granulation for biological nutrient removal from domestic wastewater. Bioresource Technology, 2012,103(1):101-108.
[20]
都叶奇,于德爽,甄建园,等.进水C/N对SNEDPR系统脱氮除磷的影响[J].环境科学, 2019,40(2):816-822. Du Y Q, Yu D S, Zhen J Y, et al. Effect of influent C/N on nitrogen and phosphorus removal in SNEDPR system[J]. Environmental Science, 2019,40(2):816-822.
[21]
甄建园,于德爽,王晓霞,等.进水C/P对SNEDPR系统脱氮除磷性能的影响[J].环境科学, 2019,40(1):343-351. Zhen J Y, Yu D S, Wang X X, et al. Effect of influent C/P on nitrogen and phosphorus removal performance of SNEDPR system[J]. Environmental Science, 2019,40(1):343-351.
[22]
王晓霞,甄建园,赵骥,等.不同污泥龄(SRT)对SNEDPR系统脱氮除磷影响[J].环境科学, 2019,40(1):352-359. Wang X X, Zhen J Y, Zhao J, et al. Effect of different sludge age (SRT) on nitrogen and phosphorus removal in SNEDPR system[J]. Environmental Science, 2019,40(1):352-359.
[23]
沈雁群,胡安辉,杨岳平.MBBR处理低C/N生活污水影响因素研究[J].浙江大学学报(理学版), 2011,38(6):671-676,681. Shen Y Q, Hu A H, Yang Y P. Study on influencingfactors of low C/N domestic sewage treatment by MBBR[J]. Journal of Zhejiang University (Science Edition), 2011,38(6):671-676,681.
[24]
Wang J, Xia L, Chen J, et al. Synergistic simultaneous nitrificationendogenous denitrification and EBPR for advanced nitrogen and phosphorus removal in constructed wetlands[J]. Chemical Engineering Journal, 2020:127605.
[25]
刘强,梁森,闫军伟,等.污泥龄对HMBR中S-EPS及膜污染的影响[J].工业水处理, 2019,39(2):34-37,41. Liu Q, Liang S, Yan J W, et al. Effect of sludge age on S-EPS and membrane fouling in HMBR[J]. Industrial Water Treatment, 2019, 39(2):34-37,41.
[26]
国家环境保护总局水和废水检测分析方法[M].北京:中国环境科学出版社, 2002:252-354. Test and analysis method for water and wastewater of State Environmental Protection Administration[M]. Beijing:China Environmental Science Press, 2002:252-354.
[27]
孙洪伟,陈翠忠,高宇学,等.碳氮比对活性污泥胞外聚合物的长期影响[J].中国环境科学, 2018,38(3):950-958. Sun H W, Chen C Z, Gao Y X, et al. Long term effect of carbon nitrogen ratio on activated sludge extracellular polymers[J]. China Environmental Science, 2018,38(3):950-958.
[28]
周俊,周立祥,黄焕忠.污泥胞外聚合物的提取方法及其对污泥脱水性能的影响[J].环境科学, 2013,34(7):2752-2757. Zhou J, Zhou L X, Huang H Z. Extraction method of extracellular polymer from sludge and its influence on sludge dewatering performance[J]. Environmental Science, 2013,34(7):2752-2757.
[29]
Aschar-Sobbi R, Abramov A Y, Diao C, et al. High sensitivity, quantitative measurements of polyphosphate using a new DAPI-based approach[J]. Journal of Fluorescence, 2008,18(5):859-866.
[30]
任世英,肖天.聚磷菌体内多聚物的染色方法[J].海洋科学, 2005,(1):59-63. Ren S Y, Xiao T. Staining method of polymer in polyphosphate accumulating bacteria[J]. Marine Science, 2005,(1):59-63.
[31]
Miao L, Wang S, Li B, et al. Effect of carbon source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate[J]. Water Research, 2016,100:405-412.
[32]
夏雪.反硝化除磷系统中碳源对除磷效果及菌群结构的影响研究[D].哈尔滨:哈尔滨工业大学, 2013. Xia X. Study on the influence of carbon source on phosphorus removal effect and bacterial community structure in denitrifying phosphorus removal system[D]. Harbin:Harbin Institute of Technology, 2013.
[33]
闫建平.内源反硝化过程N2O释放特性及影响因素研究[D].西安:西安建筑科技大学, 2017. Yan J P. Study on N2O release characteristics and influencing factors in endogenous denitrification process[D]. Xian:Xian University of Architecture and Technology, 2017.