强化反硝化除磷的新型多级缺氧-好氧工艺

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (554 KB) HTML (0 KB)
输出: BibTeX | EndNote (RIS)

摘要基于多级缺氧-好氧(MAO)工艺和反硝化除磷理论,设计一种具有反硝化除磷功能的新型MAO工艺(DPR-MAO).实验探究了该工艺的脱氮除磷效能以及各反应池的微生物群落特征.工艺运行结果表明,稳定期COD、TN、NH₄⁺-N和TP的平均出水浓度分别为7.07,9.04,0.34,和0.49mg/L,平均去除率分别为98%、87%、99%和93%.出水水质满足《城镇污水处理厂污染物排放标准》(GB 18918-2002)一级A标准.高通量测序结果表明,各反应池中微生物以变形菌门(Proteobacteria)、拟杆菌门(Bacteroidota)和绿弯菌门(Chloroflexi)为主,分别占61.85% ~ 75.58%、16.39% ~ 22.60%、1.52% ~ 4.76%.通过属水平的研究进一步分析发现,Thiothrix、Comamonas、Candidatus Accumulibacter、和Pseudomonas是具有反硝化除磷功能的优势菌属.DPR-MAO工艺实现了反硝化聚磷菌的富集以及污水中氮磷的高效去除.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	卢瑞朋
	徐文江
	李安峰
	董娜

关键词 ：反硝化除磷, 多级缺氧-好氧, 脱氮除磷, 高通量测序, 微生物群落

Abstract：Based on the multistage anoxic-oxic (MAO) process and the theory of denitrifying phosphorus removal, a noval MAO process (DPR-MAO) with ability of denitrifying phosphorus removal was designed. In this study, the nitrogen and phosphorus removal performance and the microbial community characteristics of each reaction tank were investigated. During period of stable operation, the average COD, TN, NH₄⁺-N and TP concentration in effluent were 7.07, 9.04, 0.34 and 0.49mg/L, respectively. Meanwhile, the average removal efficiecny of COD, TN, NH₄⁺-N and TP were 98%, 87%, 99% and 93%, respectively. The effluent quality of DPR-MAO process could meet the first A level of the "Discharge standard of pollutants for municipal wastewater treatment plant” (GB 18918~2002). High-throughput sequencing results suggested that Proteobacteria, Bacteroidetes and Chloroflexi were the dominant phyla in each reaction tank, accounting for 61.85% ~ 75.58%、16.39% ~ 22.60% and 1.52% ~ 4.76% of the total phyla, respectively. Further analysis at the genus level found that Thiothrix, Comamonas, Candidatus Accumulibacter and Pseudomonas were the dominant genus for denitrifying phosphorus removal. The DPR-MAO process realized the enrichment of denitrifying phosphorus accumulating organisms and the efficient removal of nitrogen and phosphorus from wastewater.

Key words： denitrifying phosphorus removal multistage anoxic-oxic nitrogen and phosphorus removal microbial community high throughput sequencing

收稿日期: 2021-09-27

PACS:

X703.1

基金资助:北京市生态环境保护科学研究院基金资助项目(Y2020-004)

通讯作者: 李安峰,研究员,lianfeng@cee.cn E-mail: lianfeng@cee.cn

作者简介: 卢瑞朋(1995-),男,河南洛阳人,北京市生态环境保护科学研究院硕士研究生,主要从事水污染控制方向技术研发及应用.

引用本文:

卢瑞朋, 徐文江, 李安峰, 董娜. 强化反硝化除磷的新型多级缺氧-好氧工艺[J]. 中国环境科学, 2022, 42(4): 1706-1713. LU Rei-peng, XU Wen-jiang, LI An-feng, Dong Na. Enhanced denitrifying phosphorus removal in novel multistage anoxic-oxic process. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(4): 1706-1713.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I4/1706

[1]	Li J, Wang Y Y, Liu J, et al. Intensified nitrogen removal by endogenous denitrification in a full-scale municipal wastewater treatment plant[J]. Environmental Research, 2022,205:112564.
[2]	Preisner M, Neverova E, Kowalewski Z. Analysis of eutrophication potential of municipal wastewater[J]. Water Science & Technology, 2020,81(9):1994-2003.
[3]	Zhang J B, Shao Y T, Wang H C, et al. Current operation state of wastewater treatment plants in urban China[J]. Environmental Research, 2021,195:110843.
[4]	Yuan L M, Zhang C Y, Xu J Y, et al. Performance of an innovative step-feed An-M(A/O)-MBR process for nutrients removal[J]. Desalination and Water Treatment, 2015,10(55):2728-2733.
[5]	王舜和,李朦,郭淑琴.多级AO与多模式AAO工艺在污水厂的应用对比[J]. 中国给水排水, 2018,34(10):48-57. Wang S H, Li M, Guo S Q. Application and Comparison between Multistage AO and multi-mode AAO in Wastewater Treatment Plant[J]. China water and wastewater, 2018,34(10):48-57.
[6]	Ge S J, Peng Y Z, Wang S Y, et al. Enhanced nutrient removal in a modified step feed process treating municipal wastewater with different inflow distribution ratios and nutrient ratios[J]. Bioresource Technology, 2010,101:9012-9019.
[7]	Vaiopoulou E, Aivasidis A. A modified UCT method for biological nutrient removal:Configuration and performance[J]. Chemosphere, 2008,72:1062-1068.
[8]	刘加强,张建昆,李莹,等.倒置A2/O+A/O工艺用于某城市污水厂二期扩建工程[J]. 中国给水排水, 2018,34(22):55-58. Liu J Q, Zhang J K, Li Ying, et al. Application of reversed A2/O + A/O process in the second-phase extension project of a municipal wastewater treatment plant[J]. China water and wastewater, 2018, 34(22):55-58.
[9]	贾建伟,党晓宏,李建洋,等.AMAO(多级AO)工艺在污水厂扩建工程中的应用及运行[J]. 中国给水排水, 2019,35(4):63-66. Jia J W, Dang X H, Li J Y, et al. Application and operation of AMAO technology (multi-stage AO) for expansion project of wastewater treatment plant[J]. China water and wastewater, 2019,35(4):63-66.
[10]	李彤彤,李英军,徐剑锋,等.多级AO工艺强化城市生活污水再生过程的污染物去除效果研究[J]. 环境工程技术学报, 2018,8(4):420- 428. LI T T, LI Y J, XU J F, et al.Analyses on pollutants removal in municipal wastewater reclamation plant with multistage AO process[J]. Journal of Environmental Engineering Technology, 2018,8(4):420- 428.
[11]	Huang X, Dong W Y, Wang H J, et al. Biological nutrient removal and molecular biological characteristics in an anaerobic-multistage anaerobic/oxic (A-MAO) process to treat municipal wastewater[J]. Bioresource Technology, 2017,241:969-978.
[12]	杨少斌,刘凯.西安市第二污水处理厂升级改造工程设计[J]. 中国给水排水, 2016,32(10):48-51. Yang S B, Liu K. Design of upgrading and reconstruction project of second WWTP in Xi'an City[J]. China water and wastewater, 2016, 32(10):48-51.
[13]	Kuba T, Smolders G, Loosdrecht M C M, et al. Biological phosphorus removal from wastewater by anaerobic-anoxic sequencing batch reactor[J]. Water science and Technology, 1993,27(5/6):241-252.
[14]	韦佳敏,刘文如,程洁红,等.反硝化除磷的影响因素及聚磷菌与聚糖菌耦合新工艺的研究进展[J]. 化工进展, 2020,39(11):4608-4618. Wei J M, Liu W R, Cheng J H, et al. Influencing factors of denitrifying phosphorus removal and advance research on novel process of coupling PAOs and GAOs[J]. Chemical Industry and Engineering Progress, 2020,39(11):4608-4618.
[15]	王军一,李伟光.反硝化除磷工艺研究进展[J]. 山东建筑大学学报, 2015,30(3):271-276. Wang J Y, Li W G. Technology and research progress of denitrifying dephosphataion[J]. Journal of Shandong Jianzhu University, 2015, 30(3):271-276.
[16]	Loosdrecht M C M, Brandse F A, Vries A C. Upgrading of waste water treatment processes for integrated nutrient removal-The BCFS® process[J]. Water Science Technology, 1998,37(9):209-217.
[17]	Bortone G, Saltarelli R, Alonso V, et al. Biological anoxic phosphorus removal-the DEPHANOX process[J]. Water Science Technology. 1996,34(1/2):119-128.
[18]	Hao X D, Loosdrecht M C M, Meijer S C F, et al. Model-based evaluation of two BNR process-UCT and A2N[J]. Water Research, 2001,35(12):2851-2860.
[19]	胡筱敏,李微,刘金亮,等. pH对以亚硝酸盐为电子受体反硝化除磷的影响[J]. 中南大学学报(自然科学版), 2013,44(5):2144-2149. Hu X M, Li W, Liu J L, et al. Influence of pH on denitrifying phosphorus removal using nitrite as electron acceptor[J]. Journal of Central South University (Science and Technology), 2013,44(5):2144- 2149.
[20]	王晓玲.MUCT工艺缺氧吸磷性能强化技术研究[D]. 哈尔滨:哈尔滨工业大学, 2010. Wang X L. Anoxic phosphorus uptake performance strengthening technology in MUCT process[D]. Harbin:Harbin Institute of Technology, 2010.
[21]	18918-2002城镇污水处理厂污染物排放标准[S]. 18918-2002 Discharge standard of pollutants for municipal wastewater treatment plant[S].
[22]	DB11/809-2012城镇污水处理厂水污染物排放标准[S]. DB11/809-2012 Discharge standard of water pollutants for municipal wastewater treatment plants[S].
[23]	赵伟华,王梅香,李健伟,等. A2O工艺和A2O+BCO工艺的脱氮除磷性能比较[J]. 中国环境科学, 2019,39(3):994-999. Zhao W H, Wang M X, Li J W, et al. Nitrogen and phosphorus removal performance comparison between A2O and A2O+BCO system[J]. China Environmental Science, 2019,39(3):994-999.
[24]	刘文龙,彭轶,苗圆圆,等.脉冲SBR工艺短程脱氮的实现及对除磷的强化[J]. 中国环境科学, 2014,34(12):3062-3069. Liu W L, Peng Y, Miao Y Y, et al. Achievement of short-cut denitrification and enhancement of phosphorus removal in a SAOSBR process[J]. China Environmental Science, 2014,34(12):3062-3069.
[25]	金鹏康,郑未元,王先宝,等.倒置A2/O与常规A2/O工艺除磷效果对比[J]. 环境工程学报, 2015,9(2):501-505. Jin P K, Zheng W Y, Wang X B, et al. Comparison of phosphorus removal efficiency between reversed and conventional A2/O processes[J]. Chinese Journal of Environmental Engineering, 2015,9(2):501- 505.
[26]	Hu J Y, Ong S L, Ng W J, et al. A new method for characterizing denitrifying phosphorus removal bacteria by using three different types of electron acceptors[J]. Water Research, 2003,37:3463-3471.
[27]	徐坚,暴瑞玲,刘清,等.反硝化除磷工艺的影响因素分析[J]. 当代化工, 2020,49(3):729-732. Xu J, Bao R L, Liu Q, et al. Analysis on Influencing Factors of Denitrifying Phosphorus Removal Process[J]. Contemporary Chemical Industry, 2020,49(3):729-732.
[28]	缪新年,汪倩,郭凯成,等.ABR-MBR耦合工艺启动及优化反硝化除磷性能[J]. 环境科学, 2020,41(9):4150-4160. Miao X N, Wang Q, Guo K C. Start-up and optimization of denitrifying phosphorus removal in ABR-MBR coupling process[J]. Environmental Science, 2020,41(9):4150-4160.
[29]	赵诗惠,吕亮,蒋志云,等.ABR-MBR组合工艺短程硝化过程的微生物种群[J]. 中国环境科学, 2018,38(2):566-573. Zhao S H, Lv L, Jiang Z Y, et al. Analysis of microbial population of shortcut nitrification in ABR-MBR process[J]. China Environmental Science, 2018,38(2):566-573.
[30]	蒋志云,韦佳敏,缪新年,等.ABR-MBR工艺反硝化除磷微生物群落特征分析[J]. 环境工程学报, 2019,13(7):1653-1661. Jiang Z Y, Wei J M, Miao X N, et al. Analysis of microbial community characteristics of denitrifying phosphorus removal in the ABR-MBR process[J]. Chinese Journal of Environmental Engineering, 2019, 13(7):1653-1661.
[31]	刘晖,孙彦富,周康群,等.PCR-DGGE法研究Sludge biomembrane (SB)系统中反硝化聚磷菌的变化[J]. 中南大学学报, 2011,42(4):1167-1174. Liu H, Sun Y F, Zhou K Q, et al. Change of denitrifying phosphate bacteria(DPB) in sludge bio-membrane system using PCR-DGGE method[J]. Journal of Central South University (Science and Technology), 2011,42(4):1167-1174.
[32]	杨浩,张国珍,杨晓妮,等.16S rRNA高通量测序研究集雨窖水中微生物群落结构及多样性[J]. 环境科学, 2017,38(4):1704-1716. Yang H, Zhang G Z, Yang X N, et al. Microbial community structure and diversity in cellar water by 16S rRNA high-throughput sequencing[J]. Environmental Science, 2017,38(4):1704-1716.
[33]	Zou G, Papirio S, Hullebusch E D, et al. Fluidized-bed denitrification of mining water tolerates high nickel Concentrations[J]. Bioresource Technology, 2015,179:284-290.
[34]	Okoro C C, Amund O O. Microbial community structure of a low sulfate oil producing facility indicate dominance of oil degrading/nitrate reducing bacteria and Methanogens[J]. Petroleum Science and Technology, 2017,0(0):1-9.
[35]	Cech J S, Hartman P. Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems[J]. Water Research, 1993,27(7):1219- 1225.
[36]	Oehmen A, Carvalho G, Lopez-vazquez C M, et al. Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Water Research, 2010,44(17):4992-5004.
[37]	Wang X, Wang S, Xue T, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015,77:191-200.
[38]	Wang X, Wang S, Zhao J, et al. Combining simultaneous nitrification-endogenous denitrification and phosphorus removal with post-denitrification for low carbon/nitrogen wastewater treatment[J]. Bioresource Technology, 2016,220:17-25.
[39]	Rubio-Rincon F J, Lopez-Vazquez C M, Welles L, et al. Cooperation between Candidatus competibacter and Candidatus accumulibacter clade I, in denitrification and phosphate removal processes[J]. Water Research, 2017,120:156-164.
[40]	Wang X, Zhao J, Yu D, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019,355:560-571.
[41]	Fan Z W, Zeng W, Wang B G, et al. Microbial community at transcription level in the synergy of GAOs and Candidatus Accumulibacter for saving carbon source in wastewater treatment[J]. Bioresource Technology, 2020,297:122454.
[42]	Wang S P, Li Z, Wang D, et al. Performance and population structure of two carbon sources granular enhanced biological phosphorus removal systems at low temperature[J]. Bioresource Technology, 2019, 300:122683.
[43]	Fang H H, Zhang T, et al. Characterization of an acetate-degrading sludge without intracellular accumulation of polyphosphate and glycogen[J]. Water Research, 2002,36:3211-3218.
[44]	Barak Y, Rijn J.Relationship between nitrite reduction and active phosphate uptake in the phosphate-accumulating denitrifier Pseudomonas sp.strain JR 12[J]. Applied and Environmental Microbiology, 2000,66(12):5236-5240.