Ecotoxicological effects and mechanism of algae on Daphnia Magna's resistance to pesticides
HE Yan1, SHI Wan-qi1, PENG Xing-xing1, YANG Wei1, LI Zhe-xing1, WEN Xin1, LUO Jun-xiao1, LIU Hua-zu1, LI Wei1,2
1. College of Environment and Ecology, Chongqing University, Chongqing 400045, China; 2. Key Laboratory of the Three Gorges Reservoir Rigon's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China
Abstract：To explore the effects and mechanisms of algae on Daphnia magna's resistance to pesticides, five algal species (Pyrrophyta, Bacillariophyta, Chlorophyta, Cryptophyta and Cyanophyta) were fed in equal amount to zooplankton Daphnia magna, which was then exposed to two insecticides: imidacloprid (water-soluble) and esfenvalerate (fat-soluble). The median lethal concentration (LC50), stoichiometric ratio (P:C, N:C) and fatty acid content of Daphnia magna were determined. The results show that, compared with biogenic chemical elements such as nitrogen and phosphorus, N3 unsaturated fatty acids can better reveal the predation of Daphnia magna on planktonic algae. The content of N3 unsaturated fatty acids in algae was a key factor affecting the resistance of Daphnia magna to water-soluble pesticides. The difference in Daphnia magna resistance was more obvious to fat-soluble pesticides than to water-soluble pesticides. Therefore, the N3 unsaturated fatty acids is the best predictor of the resistance of Daphnia magna to water-soluble pesticides, and can be used to monitor the resistance and risk of Daphnia magna food chain to pesticides in a natural environment.
何妍, 石琬绮, 彭鑫鑫, 杨巍, 李锗镡, 文昕, 罗竣潇, 刘华祖, 李伟. 藻类对大型溞抵抗农药的毒理学影响及其机制[J]. 中国环境科学, 2022, 42(9): 4416-4422.
HE Yan, SHI Wan-qi, PENG Xing-xing, YANG Wei, LI Zhe-xing, WEN Xin, LUO Jun-xiao, LIU Hua-zu, LI Wei. Ecotoxicological effects and mechanism of algae on Daphnia Magna's resistance to pesticides. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4416-4422.
Münze R, Hannemann C, Orlinskiy P, et al. Pesticides from wastewater treatment plant effluents affect invertebrate communities [J]. The Science of the Total Environment, 2017,599:387-399.
[2]
Bjergager M A, Hanson M L, Lissemore L, et al. Synergy in microcosms with environmentally realistic concentrations of prochloraz and esfenvalerate [J]. Aquatic Toxicology, 2011,101(2):17-24.
[3]
Iwasa T, Motoyama N, Ambrose J, et al. Mechanism for the differential toxicity of neonicotinoid insecticides in the honey bee, Apis mellifera [J]. Crop Protection, 2003,23(5):371-378.
[4]
张 琪,赵 成,卢晓霞,等.新烟碱类杀虫剂对非靶标生物毒性效应的研究进展 [J]. 生态毒理学报, 2020,15(1):56-71. Zhang Q, Zhao C, Lu X X, et al. Advances in research on toxic effects of neonicotinoid insecticides on non-target organisms [J]. Asian Journal of Ecotoxicology, 2020,15(1):56-57.
[5]
李田田,郑珊珊,王 晶,等.新烟碱类农药的污染现状及转化行为研究进展 [J]. 生态毒理学报, 2018,13(4):9-21. Li T T, Zheng S S, Wang J, et al. A review on occurence and transformation behaviors of neonicotinoid pesticides [J]. Asian Journal of Ecotoxicology, 2018,13(4):9-21.
[6]
Long E Y, Krupke C H. Non-cultivated plants present a season-long route of pesticide exposure for honey bees [J]. Nature Communications, 2016,7(5):637-646.
[7]
Hallmann C A, Foppen R, Van T, et al. Declines in insectivorous birds are associated with high neonicotinoid concentrations [J]. Nature, 2014,511(7509):341-343.
[8]
Masumi Y, Takashi K, Hiroshi K, et al. Neonicotinoids disrupt aquatic food webs and decrease fishery yields [J]. Science, 2019,366(6465): 620-623.
[9]
齐延凯,郭楠楠,宋 超,等.鲥鯸淀水中有机农药污染特征及生态风险评价 [J]. 环境科学与技术, 2019,42(9):126-133. Qi Y K, Guo N N, Song C, et al. Pollution characteristics and ecological risk assessment of organic pesticides in the water environment of Shihoudian Lake [J]. Environmental Science & Technology, 2019,42(9):126-133.
[10]
赵 晨,彭书传,陈天虎,等.巢湖流域和太湖流域沉积物中苄氯菊酯和高效氰戊菊酯的生态风险评价 [J]. 环境科学学报, 2016,36(3): 1080-1091. Zhao C, Peng S C, Chen T H, et al. Ecological risk assessment of sediment-associated permethrin and esfenvalerate in Chaohu Lake and Taihu Lake watersheds [J]. Acta Scientiae Circumstantiae, 2016,36(3):1080-1091.
[11]
Delnat V, Tran T, Janssens L, et al. Daily temperature variation magnifies the toxicity of a mixture consisting of a chemical pesticide and a biopesticide in a vector mosquito [J]. Science of the Total Environment, 2019,659:33-40.
[12]
Naeem S, Matthias L, Saskia K. Environmental stress increases synergistic effects of pesticide mixtures on daphnia magna [J]. Environmental Science & Technology, 2019,53(21):12586-12593.
[13]
William K, Martínez C. Chemical Ecology of Food [M]. Princeton University Press, 2007:47-114.
[14]
Thomas O, Jeroen O, Jimmy F, et al. Digestive capacity and toxicity cause mixed diets in red knots that maximize energy intake rate [J]. The American Naturalist, 2014,183(5):650-659.
[15]
Dawson P, Curran J. Technical note A new technique for interpolating the reflectance red edge position [J]. International Journal of Remote Sensing, 1998,19(11):2133-2139.
[16]
Persson J, Fink P, Goto A, et al. To be or not to be what you eat: regulation of stoichiometric homeostasis among autotrophs and heterotrophs [J]. Oikos, 2010,119(5):741-751.
[17]
谢 锦,常顺利,张毓涛,等.天山北坡植物土壤生态化学计量特征的垂直地带性 [J]. 生态学报, 2016,36(14):4363-4372. Xie J, Chang S L, Zhang Y T, et al. Plant and soil ecological stoichiometry with vertical zonality on the northern slope of the middle Tianshan Mountains [J]. Acta Ecologica Sinica, 2016,36(14):4363-4372.
[18]
蔡永久,薛庆举,陆永军,等.长江中下游浅水湖泊5种常见底栖动物碳、氮、磷化学计量特征 [J]. 湖泊科学, 2015,27(1):76-85. Cai Y J, Xue Q J, Lu Y J, et al. C:N:P stoichiometry of five common macrozoobenthic taxa in shallow lakes along the Yangtze River [J]. Lake Sciences, 2015,27(1):76-85.
[19]
陈 蕾,李超伦.海洋浮游生物的生态化学计量学研究进展 [J]. 应用生态学报, 2014,25(10):3047-3055. Chen L, Li C L. Research advances in ecological stoichiometry of marine plankton [J]. Chinese Journal of Applied Ecology, 2014,25(10): 3047-3055.
[20]
Guillou H, Martin P, Pineau T. Transcriptional regulation of hepatic fatty acid metabolism [J]. Sub-cellular Biochemistry, 2008,49:3-47.
[21]
Wiktorowska A, Berezińska M, Nowak Z. PUFAs: Structures, metabolism and functions [J]. Advances in Clinical and Experimental Medicine: official organ Wroclaw Medical University, 2015,24:931-941.
[22]
晁红娟,雷占兰,刘爱琴,等.Omega-3多不饱和脂肪酸性质、功能及主要应用 [J]. 中国食品添加剂, 2019,30(10):122-130. Chao H J, Lei Z L, Liu A Q, et al. Properties, functions and main applications of Omega-3polyunsaturated fatty acids [J]. China Food Additives, 2019,30(10):122-130.
[23]
Twining C, Brenna T, Hairston N, et al. Highly unsaturated fatty acids in nature: what we know and what we need to learn [J]. Oikos, 2016,125(6):749-760.
[24]
Hixson S, Sharma B, Kainz M, et al. Production, distribution, and abundance of long-chain omega-3polyunsaturated fatty acids: a fundamental dichotomy between freshwater and terrestrial ecosystems [J]. NRC Research Press, 2015,23(4):253-262.
[25]
Shchepinov, Roginsky, et al. Deuterium protection of polyunsaturated fatty acids against lipid peroxidation: anovel approach to mitigating mitochondrial neurological diseases. In: Omega-3Fatty Acids in Brain and Neurological Health, 2014:373-383.
[26]
张志超,余新威,方 力,等.浙东渔场海产品中EPA和DHA含量分析 [J]. 中国卫生检验杂志, 2015,25(7):1046-1048. Zhang Z C, Yu X W, Fang L, et al. Eicosapentaenoic acid and Docosahexaenoic acid content analysis of marine products from the East Zhejiang Fishery [J]. Chinese Journal of Health Laboratory Technology, 2015,25(7):1046-1048.
[27]
张东平,张少欢,余应新,等.太湖鱼中多不饱和脂肪酸及其与多氯联苯共摄入益害分析 [J]. 科学通报, 2012,57(5):324-331. Zhang D P, Zhang S H, Yu Y X, et al. Polyunsaturated fatty acids in fish from Taihu Lake and the associated risk of ingesting polychlorinated biphenyls [J]. Chinese Science Bulletin, 2012,57(5):324–331.
[28]
张红霞,张加玲,尚晓虹,等.中国黄海海域部分海鱼脂肪酸含量分析 [J]. 卫生研究, 2014,43(3):423-429. Zhang H X, Zhang J L, Shang X H, et al. Fatty acids content of common marine fish from Yellow Sea of China [J]. Journal of Hygiene Research, 2014,43(3):423-429.
[29]
Nevigato T, Masei M, Orban E, et al. Analysis offatty acids in 12Mediterranean fish species:advantages an d limitations of a new GC-FID/GC-MS based echnique [J]. Lipids, 2012,47(7):741-753.
[30]
Bayraktar A. OECD (Organisation for Economic Cooperation and Development) and environment. [J]. Endocrinology, 2010,138(10):200-207.
[31]
许 静,王晓昌,马晓妍.酵母提取物对大型蚤急性毒性实验的影响 [J]. 环境工程学报, 2015,9(1):485-490. Xu J, Wang X C, Ma X Y. Impact of yeast extract on acute toxicity test using Daphnia magna [J]. Chinese Journal of Environmental Engineering, 2015,9(1):485-490.
[32]
Kilham S, Kreeger D, Lynn S, et al. COMBO: a defined freshwater culture medium for algae and zooplankton [J]. Hydrobiologia, 1998, 377:1-3.
[33]
Bowman M, Oiler W, Cairns W, et al. Stressed bioassay systems for rapid screening of pesticide residues. Part I: Evaluation of bioassay systems [J]. Archives of Environmental Contamination and Toxicology, 1981,10(1).
[34]
杨光蓉,豆鹏鹏,马 瑜,等.金佛山亚热带常绿阔叶林地表土壤动物群落特征及其影响因素 [J]. 生态学报, 2020,40(21):7602-7610. Yang G R, Dou P P, Ma Y, et al. Characteristics and influencing factors of surface soil fauna community in a subtropical evergreen broad- leaved forest of Jinfo Mountsin [J]. Acta Ecological Sinica, 2020, 40(21):7602-7610.
[35]
豆鹏鹏,王 芳,马 瑜,等.叶凋落物碳、氮和磷元素对模拟淋溶的响应 [J]. 科学通报, 2018,63(30):3114-3123. Dou P P, Wang F, Ma Y, et al. Response of litter carbon, nitrogen and phosphorus to simulated leaching [J]. Chinese Science Bulletin, 2018, 63:3114-3123.
[36]
Li W, Xu X G, Yao J M, et al. Combined effects of elevated carbon dioxide and temperature on phytoplankton-zooplankton link: A multi- influence of climate change on freshwater planktonic communities [J]. The Science of the Total Environment, 2019,658:1175-1185.
[37]
Sakdullah A, Makoto T. One-step method for quantitative and qualitative analysis of fatty acids in marine animal samples [J]. Journal of Experimental Marine Biology and Ecology, 2007,354(1):1-8.
[38]
Reynolds C. What factors influence the species composition of phytoplankton in lakes of different trophic status [J]. Hydrobiologia, 1998,369-370:11-26.
[39]
Sabine G. N: P ratios in terrestrial plants: variation and functional significance [J]. The New Phytologist, 2004,164(2):243-266.
[40]
Field C, Mooney H. The photosynthesis-nitrogen relationship in wild plants. In: Givnish TJ ed [M]. UK: Cambridge University Press, 1986:25-55.
[41]
Yuste J, Baldocchi D, Gershenson A, et al. Microbial soil respiration and its dependency on carbon inputs, soil temperature and moisture. Global Change Biology, 2007,13(9):2018-2035..
[42]
Müller-Navarra D C, Brett M T, Liston A M, et al. A highly unsaturated fatty acid predicts carbon transfer between primary producers and consumers [J]. Nature, 2000,403(6765):74.
[43]
Müller-Navarra D C, Brett M T, Park S, et al. Unsaturated fatty acid content in seston and trophodynamic coupling in lakes [J]. Nature, 2004,427(6969):69-72.
[44]
Twining C, Shipley J, Winkler D. Aquatic insects rich in omega3fatty acids drive breeding success in a widespread bird [J]. Ecology Letters, 2018,21(12):1812-1820.
[45]
路宗博,张盈月,葛科立,等. n-3多不饱和脂肪酸对下丘脑神经干细胞来源的外泌体miRNA的调节 [J]. 食品科学, 2021,12:1-9. Lu Z B, Zhang Y Y, Ge K L, et al. Regulation of miRNA in exosome derived from hypothalamic neural stem cells by n-3polyunsaturated fatty acids [J]. Food Science, 2021,12:1-9.
[46]
An W Q, He H L, Dong X H, et al. Regulation of growth, fatty acid profiles, hematological characteristics and hepatopancreatic histology by different dietary n-3highly unsaturated fatty acids levels in the first stages of juvenile Pacific white shrimp (Litopenaeus vannamei) [J]. Aquaculture Reports, 2020,17:20-28.
[47]
Twining C, Brenna T, Lawrence P, et al. Omega-3long-chain polyunsaturated fatty acids support aerial insectivore performance more than food quantity [J]. Proceedings of the National Academy of Sciences, 2016,113(39):7347-7349.
[48]
张 稳,谢奉军,金 敏,等.饲料中n-3高不饱和脂肪酸含量对三疣梭子蟹幼蟹生长性能及脂肪酸组成的影响 [J]. 动物营养学报, 2014,26(5):1254-1264. Zhang W, Xie F J, Jin M, et al. Effects of n-3high unsaturated fatty acid content in feed on juveniles of Portunus trituratus Effects of Growth Performance and Fatty Acid Composition [J]. Chinese Journal of Animal Nutrition, 2014,26(5):1254-1264.
[49]
Tian J J, Ji H, Hiromi O, et al. Effects of dietary arachidonic acid ( ARA ) on lipid metabolism and health status of juvenile grass carp, Ctenopharyngodon idellus [J]. Aquaculture, 2014,430:57-65.
[50]
刘志国,王华林,王丽梅,等.多不饱和脂肪酸对神经细胞保护作用的研究进展 [J]. 食品科学, 2016,37(7):239-248. Liu Z G, Wang H L, Wang L M, et al. Advances in research on neuron-protective role of polyunsaturated fatty acids [J]. Food Science, 2016,37(7):239-248.
[51]
Nakamura M, Yudell B, Loor J. Regulation of energy metabolism by long-chain fatty acids [J]. Progress in Lipid Research, 2014,53(1): 124-144.
[52]
Dariush M, Wu J. (n-3) fatty acids and cardiovascular health: Are effects of EPA and DHA shared or complementary [J]. The Journal of Nutrition, 2012,142(3):614-625.
