Effect of electric field on structure of fulvic acid during sludge composting
TAN Zhi-han1,2, SUN Xiao-jie1,2, Xi Bei-dou1,3, LU Xue-shuang1,2, LI Qiu-hong1,2, MO Jing-jing1,2, Zhang Jun1,2
1. Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China; 2. Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology for Science and Education Combined with Science and Technology Innovation Base, Guilin 541004, China; 3. State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Abstract：Fulvic acid (FA) is an important component of compost-derived humic substances, and its structure and electron transfer capacity are vital factors causing environmental effects. In order to investigate the influence of electric field on structural characteristics and electron transfer capacity (ETC) of FA that was formed during sludge composting, municipal sludge and rice bran were applied as raw materials, and two composting experiments, i.e.,one with a 5V direct current electric field and the other with no electric field (CK),were set up. Excitation-emission matrix florescence spectra coupled with parallel factor analysis, ultraviolet and visible spectroscopy, and Fourier transform infrared spectroscopy combined with two-dimensional correlation analysis were employed to investigate the evolution of the structure and composition of FA during composting. In addition, electrochemical methods was applied to determine the electron accepting capacities (EAC) and electron donating capacities (EDC) of the compost-derived FA. The results revealed that the major components of compost-derived FA were tryptophane-like, fulvic-like, and humic-like substances. Tryptophane-like substances in FA decreased during composting, whereas fulvic-like and humic-like substances increased during the process. Notably, the electric field promoted the degradation of tryptophan-like substances and the formation of humic-like substances, which inceased the aromatic degree, molecular weight, and humification of the FA. Results obtained from electrochemical analysis showed that the ETC of compost-derided FA increased initially and then decreased, Compared to the CK treatment, electric field application enhanced the EAC of compost-derided FA, though it reduced the EDC of FA, resulting the ETC of the FA from the later stage of composing was the highest. These results facilitated to elucidate the formation process of the FA and its redox properties during sludge composting.
谭知涵, 孙晓杰, 席北斗, 卢雪霜, 李秋虹, 莫晶晶, 张军. 电场对污泥堆肥富里酸结构特征的影响[J]. 中国环境科学, 2023, 43(1): 244-254.
TAN Zhi-han, SUN Xiao-jie, Xi Bei-dou, LU Xue-shuang, LI Qiu-hong, MO Jing-jing, Zhang Jun. Effect of electric field on structure of fulvic acid during sludge composting. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(1): 244-254.
崔东宇,何小松,席北斗,等.堆肥腐熟前后胡敏酸与富里酸的还原容量比较[J]. 中国环境科学, 2015,35(7):2087-2094. Cui D Y, He X S, Xi B D, et al. The comparison of reduction capacity between humic acid and fulvic acid extracted from the compost[J]. China Environmental Science, 2015,35(7):2087-2094.
[2]
张传严,席北斗,张强,等.堆肥在土壤修复与质量提升的应用现状与展望[J]. 环境工程, 2021,39(9):176-186. Zhang C Y, Xi B D, Zhang Q, et al. Application status and prospect of compost in soil remediation and quality improvement[J]. Environmental Science, 2021,39(9):176-186.
[3]
Tang J, Li X, Zhao W, et al. Electric field induces electron flow to simultaneously enhance the maturity of aerobic composting and mitigate greenhouse gas emissions[J]. Bioresource Technology, 2019, 279:234-242.
[4]
Cao Y, Wang X, Zhang X, et al. An electric field immobilizes heavy metals through promoting combination with humic substances during composting[J]. Bioresource Technology, 2021,330:124996.
[5]
付涛,李翔,上官华媛,等.电场促进畜禽粪便好氧堆肥中DOM演化的光谱学研究[J]. 环境科学学报, 2021,41(4):1465-1477. Fu T, Li X, Shangguan H Y, et al. Spectrum analysis of the evolution of DOM during electric-field assisted aerobic composting for the treatment of livestock and poultry manure treatment[J]. Acta Scientiae Circumstantiae, 2021,41(4):1465-1477.
[6]
刘厶瑶,李玉双,侯永侠,等.富里酸对土壤中DnBP的降解及微生物活性的影响[J]. 农业环境科学学报, 2020,39(2):313-320. Liu Si-yao, Li Yu-shuang, Hou Yong-xia, et al. Effects of fulvic acid on degradation of DnBP and microbial activity in soil[J]. Journal of Agro-Environment Science, 2020,39(2):313-320.
[7]
Aranganathan L, Radhika Rajasree S R, Govindaraju K, et al. Spectral and microscopic analysis of fulvic acids isolated from marine fish waste and sugarcane bagasse co-compost[J]. Biocatalysis and Agricultural Biotechnology, 2020,29:101762.
[8]
张强,席北斗,杨津津,等.不同物料堆肥富里酸的结构特征的研究[J]. 中国环境科学, 2021,41(2):763-770. Zhang Q, Xi B D, Yang J J, et al. Structural characteristics of fulvic acid composted with different materials[J]. China Environmental Science, 2021,41(2):763-770.
[9]
Yao R, Yang J, Zhu W, et al. Impact of crop cultivation, nitrogen and fulvic acid on soil fungal community structure in salt-affected alluvial fluvo-aquic soil[J]. Plant and Soil, 2021,464(1):539-558.
[10]
Qi H, Zhai W, Du Y, et al. Core bacterial community driven the conversion of fulvic acid components during composting with adding manganese dioxide[J]. Bioresource Technology, 2021,337:125495.
[11]
Jindo K, Sonoki T, Matsumoto K, et al. Influence of biochar addition on the humic substances of composting manures[J]. Waste Management, 2016,49:545-552.
[12]
宋凡浩,栗婷婷,张进,等.二维相关荧光光谱探究土壤富里酸亚组分的质子键合多相性[J]. 光谱学与光谱分析, 2019,39(10):3071- 3077. Song F H, Li T T, Zhang J, et al. Proton binding heterogeneity in soil fulvic acid sub-fractions using two-dimensional correlation Fluorescence Spectrometry[J]. Spectroscopy and Spectral Analysis, 2019,39(10):3071-3077.
[13]
Li C, Li H, Yao T, et al. Effects of swine manure composting by microbial inoculation:Heavy metal fractions, humic substances, and bacterial community metabolism[J]. Journal of Hazardous Materials, 2021,415:125559.
[14]
Aeschbacher M, Sander M, Schwarzenbach R P. Novel electrochemical approach to assess the redox properties of humic substances[J]. Environmental Science & Technology, 2010,44(1):87- 93.
[15]
陶亚,袁田,周顺桂,等.水溶性有机物电子转移能力与荧光峰强度的关系研究[J]. 环境科学, 2012,33(6):1871-1877. Tao Y, Yuan T, Zhou S G, et al. Relationship between electron transfer capacity and fluorescence characteristics of dissolved organic matter[J]. Environmental Science, 2012,33(6):1871-1877.
[16]
唐朱睿,黄彩红,檀文炳,等.基于电化学方法研究猪粪堆肥过程溶解性有机物电子转移能力演变规律[J]. 分析化学, 2018,46(3):422- 431. Tang Z R, Huang C H, Tan W B, et al. Electron transfer capacities of dissolved organic matter derived from swine manure based on eletrochemical method[J]. Chinese Journal of Analytical Chemistry, 2018,46(3):422-431.
[17]
Zhao Y, Wei Y, Zhang Y, et al. Roles of composts in soil based on the assessment of humification degree of fulvic acids[J]. Ecological Indicators, 2017,72:473-480.
[18]
He X, Xi B, Cui D, et al. Influence of chemical and structural evolution of dissolved organic matter on electron transfer capacity during composting[J]. Journal of Hazardous Materials, 2014,268:256- 263.
[19]
Stedmon C A, Bro R. Characterizing dissolved organic matter fluorescence with parallel factor analysis:a tutorial[J]. Limnology and Oceanography:Methods, 2008,6(11):572-579.
[20]
文欣,赵越,时俭红,等.多元统计分析研究不同物料堆肥富里酸紫外吸收光谱特性[J]. 环境工程学报, 2017,11(3):1814-1818. Wen X, Zhao Y, Shi J H, et al. Characteristic multivariable analysis to study UV-visble spectra properties of fulvic acids (FA) from different composts[J]. Chinese Journal of Environmental Engineering, 2017, 11(3):1814-1818.
[21]
Mangalgiri K P, Timko S A, Gonsior M, et al. PARAFAC Modeling of irradiation- and oxidation-induced changes in fluorescent dissolved organic matter extracted from poultry litter[J]. Environmental Science & Technology, 2017,51(14):8036-8047.
[22]
闫金龙,江韬,赵秀兰,等.含生物质炭城市污泥堆肥中溶解性有机质的光谱特征[J]. 中国环境科学, 2014,34(2):459-465. YAN J L, Jiang T, Zhao X L, et al. Spectral characteristics of dissolved organic matter in co-composting process of municipal sludge with biochar[J]. China Environmental Science, 2014,34(2):459-465.
[23]
Zhang X, Zhao Y, Meng H, et al. Revealing the inner dynamics of fulvic acid from different compost-amended soils through microbial and chemical analyses[J]. Journal of Agricultural and Food Chemistry, 2020,68(12):3722-3728.
[24]
李艳,魏丹,王伟,等.秸秆-牛粪发酵过程中溶解性有机质的荧光光谱特征[J]. 光谱学与光谱分析, 2021,41(9):2846-2852. Li Y, Wei D, Wang W, et al. Fluorescence spectroscopy characteristics of dissolved organic matter analysis of straw-cow dung fermentation in different proportion[J]. Spectroscopy and Spectral Analysis, 2021,41(9):2846-2852.
[25]
肖隆庚,陈文松,陈国丰,等.中国南海CDOM三维荧光光谱特征研究[J]. 环境科学学报, 2014,34(1):160-167. Xiao L G, Chen W S, Chen G F, et al. Fluorescence excitation-emission matrix spectroscopy of chromophoric dissolved organic matter in the South China Sea[J]. Acta Scientiae Circumstantiae, 2014,34(1):160-167.
[26]
Song C, Li M, Xi B, et al. Characterisation of dissolved organic matter extracted from the bio-oxidative phase of co-composting of biogas residues and livestock manure using spectroscopic techniques[J]. International Biodeterioration & Biodegradation, 2015,103:38-50.
[27]
李丹,何小松,高如泰,等.紫外-可见光谱研究堆肥水溶性有机物不同组分演化特征[J]. 中国环境科学, 2016,36(11):3412-3421. Li D, He X S, Gao R T, et al. Evolution based on the spectra of different hydrophilic and hydrophobic components separated from dissolved organic matter (DOM) during compost[J]. China Environmental Science, 2016,36(11):3412-3421.
[28]
Xu J, Jiang Z, Li M, et al. A compost-derived thermophilic microbial consortium enhances the humification process and alters the microbial diversity during composting[J]. Journal of Environmental Management, 2019,243:240-249.
[29]
Song C, Li M, Xi B, et al. Characterisation of dissolved organic matter extracted from the bio-oxidative phase of co-composting of biogas residues and livestock manure using spectroscopic techniques[J]. International Biodeterioration & Biodegradation, 2015,103:38-50.
[30]
Zahra El Ouaqoudi F, El Fels L, Lemée L, et al. Evaluation of lignocelullose compost stability and maturity using spectroscopic (FTIR) and thermal (TGA/TDA) analysis[J]. Ecological Engineering, 2015,75:217-222.
[31]
Li W, Zhang F, Ye Q, et al. Composition and copper binding properties of aquatic fulvic acids in eutrophic Taihu Lake, China[J]. Chemosphere, 2017,172:496-504.
[32]
Wang Q, Awasthi M K, Zhao J, et al. Improvement of pig manure compost lignocellulose degradation, organic matter humification and compost quality with medical stone[J]. Bioresource Technology, 2017,243:771-777.
[33]
Amir S, Jouraiphy A, Meddich A, et al. Structural study of humic acids during composting of activated sludge-green waste:Elemental analysis, FTIR and 13C NMR[J]. Journal of Hazardous Materials, 2010,177(1):524-529.
[34]
肖骁,何小松,席北斗,等.生活垃圾填埋富里酸电子转移能力与影响因素[J]. 环境化学, 2018,37(4):679-688. Xiao X, He X S, Xi B D, et al. Electron transfer capacity of fulvic acid and its factors during municipal solid waste landfill[J]. Environmental Chemistry, 2018,37(4):679-688.
[35]
Noda I. Close-up view on the inner workings of two-dimensional correlation spectroscopy[J]. Vibrational Spectroscopy, 2012,60:146- 153.
[36]
Yu Z, Liu X, Zhao M, et al. Hyperthermophilic composting accelerates the humification process of sewage sludge:Molecular characterization of dissolved organic matter using EEM-PARAFAC and two-dimensional correlation spectroscopy[J]. Bioresource Technology, 2019,274:198-206.
[37]
唐朱睿,席北斗,檀文炳,等.市政污泥堆肥过程胡敏酸电子转移能力的演变规律[J]. 环境化学, 2018,37(4):689-697. Tang Z R, Xi B D, Tan W B, et al. Evolution of the electron transfer capacity of humic acid during municipal sludge composting process[J]. Environmental Chemistry, 2018,37(4):689-697.
[38]
Huang W, Li Y, Liu X, et al. Linking the electron transfer capacity with the compositional characteristics of dissolved organic matter during hyperthermophilic composting[J]. Science of The Total Environment, 2021,755:142687.
