Roles of cyanobacterial-derived dissolved organic matter in mediating biodegradation of 17β-estradiol in water column
HUA Ke1, JIANG Yu2, WU Yuan-qiang1, YANG Nan1, LIU Xin1
1. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Provincial Ecological Assessment Center (Jiangsu Provincial Management Center for Emissions Registration and Exchange), Nanjing 210036, China
Abstract：The effects of cyanobacterial-derived dissolved organic matter (COM) and its microbial processing on the biodegradation of 17β-estradiol (E2) in lake water column were investigated in this study. Through the simulated microbial ageing within a four-stage plug-flow bioreactor, five COM fractions with a gradient of decreasing bioreactivity were separated: S0 (78%) > S1 (67%) > S0 (36%) > S0 (13%) > S0 (1%). Within the 60 hours of batch incubation at dark, the addition of COM fractions at 8mgC/L exhibited strong acceleration on E2biodegradation, increasing the first-order kinetic rate with the order of S0 > S4 > S3 > S1 > S2. Microbial analysis further showed that the highly labile compounds in COM not only promoted the bacterial growth but also maintained the diverse microbial community in the S0-amended group. In comparsion, the bacterial concentration and E2biodegradation rate in the S1- and S2-amended groups was remarkably lower, meaning that the semilabile and recalcitrant compounds were unable to serve as effective carbon sources. However, the enriched aromatic, humic structure in the S3- and S4-amended groups significantly increased the biomass-normalized E2biodegradation rate, possibly due to the selection of potentially E2-degrading bacteria and the activation of catabolic enzymes under carbon-limited conditions. The environmental behavior and fate of estrogens in eutrophic waters are closely related to the bioreactivity of COM.
化柯, 蒋豫, 吴元强, 杨楠, 刘新. 藻源可溶有机质对17β-雌二醇生物降解的影响[J]. 中国环境科学, 2022, 42(4): 1829-1836.
HUA Ke, JIANG Yu, WU Yuan-qiang, YANG Nan, LIU Xin. Roles of cyanobacterial-derived dissolved organic matter in mediating biodegradation of 17β-estradiol in water column. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(4): 1829-1836.
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