Effect of H2S on glucosinolate biosynthesis in Arabidopsis thaliana based on transcriptomics
QI Hong-xue1, WU Li-hua2, LI Li-hong1
1. Department of Chemistry and Chemical Engineering, Jinzhong University, Yuci 030619, China; 2. Department of Biology, Taiyuan Normal University, Yuci 030619, China
Abstract：Glucosinolates are a group of nitrogen-and sulfur-containing secondary metabolites in cruciferous plants, which are closely related to environmental factors. In this study, transcriptome sequencing of Arabidopsis treated with hydrogen sulfide (H2S) was performed, and the biological functions and metabolic pathways of differentially expressed genes were analyzed to explore the regulatory effect of H2S on glucosinolate biosynthesis in plants. The results showed that a total of 3160 genes were differentially expressed in Arabidopsis seedlings sprayed with 100μmol/L H2S for 3 days, including genes involved in metabolism, binding, catalysis, transcription regulation, transport and signal transduction. KEGG enrichment analysis showed that differentially expressed genes were significantly enriched in multiple primary and secondary metabolism. After exogenous H2S treatment, nine genes (8up-regulated and 1down-regulated) involved in aliphatic glucosinolates biosynthesis were identified to be differentially expressed, but genes associated with indole glucosinolates biosynthesis showed no obvious change. Meantime, several genes that participated in sulfur metabolism, cysteine and methionine metabolism, and glutathione metabolism were up-regulated, suggesting that exogenous H2S can enhance sulfur-related metabolic pathways, which would promote the aliphatic glucosinolates biosynthesis in plants. Transcription factor analysis showed that MYB29 was up-regulated, which could positively regulate the aliphatic glucosinolates biosynthesis. And MYB51, which would inhibit the aliphatic glucosinolates biosynthesis, was down-regulated. These results suggested that H2S could regulate the aliphatic glucosinolates biosynthesis in plants through MYB transcription factors. The qRT-PCR analysis of several genes involved in the glucosinolate biosynthesis verified the accuracy of transcriptomic sequencing, which further proved that H2S participated in the regulation of glucosinolate biosynthesis in plants.
祁红学, 吴丽华, 李利红. 基于转录组分析外源H2S对拟南芥芥子油苷生物合成的影响[J]. 中国环境科学, 2023, 43(2): 957-963.
QI Hong-xue, WU Li-hua, LI Li-hong. Effect of H2S on glucosinolate biosynthesis in Arabidopsis thaliana based on transcriptomics. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 957-963.
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