中国环境科学
 
 
中国环境科学  2023, Vol. 43 Issue (2): 946-956    DOI:
环境毒理与健康 最新目录| 下期目录| 过刊浏览| 高级检索 |
基于Illumina RNA-Seq分析的三种内分泌干扰物对斑马鱼神经毒性效应与机制
郭子一1, 王伟伟2, 宋杰1, 王慧利1
1. 苏州科技大学, 江苏 苏州 215009;
2. 温州医科大学, 浙江 温州 325035
Neurotoxicity effects of the three endocrine disruptors on zebrafish and the underlying molecular mechanisms by using the Illumina RNA-seq technique
GUO Zi-yi1, WANG Wei-wei2, SONG Jie1, WANG Hui-li1
1. Suzhou University of Science and Technology, Suzhou 215009, China;
2. Wenzhou Medical University, Wenzhou 325035, China

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摘要 三氯生、三氯卡班和双酚A均具内分泌干扰作用,但从转录组学层面上揭示其靶分子及神经毒性机制鲜有报道,因此,本文借助RNA-Seq测序获得它们暴露斑马鱼幼鱼的转录组数据,基于生物信息分析证明:三种药物暴毒组差异表达基因(DEGs)的GO功能和KEGG代谢途径富集在生物过程、细胞组成和分子功能上,富集的DEGs数量和功能均不同,在神经相关途径上均有富集,但具体信号通路和途径不同.PPI网络节点关联度计算发现:富集在神经通路的Hub基因也不同,且与神经标志功能基因之间存在丰富间接的互作网络.将DEGs与TCGA数据库中胶质母细胞瘤GBM突变基因整合,证实其环境暴露均有诱发GBM风险,但发生途径和调控信号通路不同,故从分子水平上解析了三种污染物诱导神经毒性机制的根源.
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郭子一
王伟伟
宋杰
王慧利
关键词 内分泌干扰物斑马鱼转录组测序神经毒性神经胶质母细胞瘤    
Abstract:Triclosan (TCS), triclocarban (TCC), and bispenol A (BPA) are all proved to possess endocrine disrupting effects; however, little data are available on their neurotoxicity effects on zebrafish (Danio rerio) and the underlying molecular mechanisms. Herein, zebrafish transcriptomic data was obtained with the aid of RNA-Seq after exposure to the aforementioned three pollutants. By means of bioinformatics analysis, nine common positively differential expression genes (DEGs) were screened in the three exposure treatments. The GO functions and KEGG pathways of nine DEGs were mainly attributable to biological process, cellular component, and molecular function, in which the number and function were different with varying chemical exposure. Notably, most of the DEGs were found to be enriched in the nerve-related pathways, but their detailed pathways were different for three chemicals. The computation of node-correlation degree of PPI network showed that the hub genes enriched in neural pathways were different in the three treatments, which had rich and indirect interaction networks with neural marker genes. By integrating DEGs with GBM mutant gene of glioblastoma in the TCGA database, we confirmed that chemical exposure induced GBM risk, but the related occurrence pathway and regulatory signaling pathway were different. Therefore, the underlying neurotoxicity mechanisms induced by the three pollutants were disclosed at the molecular level.
Key wordsendocrine disruptors    zebrafish    transcriptome sequencing    neurotoxicity    glioblastoma   
收稿日期: 2022-07-04     
PACS: X171  
基金资助:国家自然科学基金资助项目(32071617)
通讯作者: 王慧利,教授,whuili@163.com     E-mail: whuili@163.com
作者简介: 郭子一(2000-),女,山东枣庄人,苏州科技大学硕士研究生,主要从事风险评价与生态安全方面的研究.
引用本文:   
郭子一, 王伟伟, 宋杰, 王慧利. 基于Illumina RNA-Seq分析的三种内分泌干扰物对斑马鱼神经毒性效应与机制[J]. 中国环境科学, 2023, 43(2): 946-956. GUO Zi-yi, WANG Wei-wei, SONG Jie, WANG Hui-li. Neurotoxicity effects of the three endocrine disruptors on zebrafish and the underlying molecular mechanisms by using the Illumina RNA-seq technique. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 946-956.
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