不同形态微塑料和汞对斑马鱼胚胎发育毒性联合作用研究

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摘要为探讨不同形态微塑料对生物体的毒性差异,本研究比较了聚对苯二甲酸乙二醇酯(PET)颗粒(直径约70~250µm)和纤维(长度约3~5mm,直径约20µm)与重金属汞(Hg)对斑马鱼胚胎发育的联合毒性效果.结果表明,Hg单独暴露对斑马鱼胚胎造成了胚胎发育毒性(如血流发育障碍,心跳变缓和孵化率降低)以及心包囊水肿和尾巴畸形效应,48和72h的畸形率分别高达31.3%和91.7%.纤维状(f-PET)和颗粒状(p-PET)微塑料都会减轻Hg的胚胎发育毒性.从代谢组学的数据来看,Hg对机体造成的糖代谢和氨基酸代谢紊乱效应在微塑料和Hg联合暴露组也显著降低(P<0.01).但是,由于对Hg吸附能力和过程的差异,p-PET更显著缓解Hg造成的24h血流障碍效应,而在暴露后期在降低Hg对斑马鱼胚胎畸形毒性方面不如f-PET显著.因此,微塑料与Hg的联合毒性效果与微塑料的形态有关.

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	石岩松
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	端正花

关键词 ：微塑料, 形态, 汞, 斑马鱼胚胎, 联合毒性

Abstract：To explore the toxicity of microplastics with different shapes to organisms, the toxicity effects of polyethylene terephthalate (PET) granules (approximately 70~250μm in diameter) and fibers (approximately 3~5mm in length and 20μm in diameter) combined with heavy metal mercury (Hg) on zebrafish embryo development were compared in the present study. The results showed that the single exposure of Hg to zebrafish embryos resulted in embryonic development toxicity (such as blood flow development disorder, cardiac relaxation, and reduced hatching rate), pericardial sac edema, and tail malformations. The malformation rates at 48 and 72h were as high as 31.3% and 91.7%, respectively. Fibrous (f-PET) and granular (p-PET) microplastics could reduce the embryonic toxicity of Hg. From the data of metabolomics, the disorder effects of glucose metabolism and amino acid metabolism caused by Hg on zebrafish larvae were significantly reduced in combined exposure of microplastics and Hg (P< 0.01). However, due to the difference in the adsorption capacity and process of Hg, p-PET significantly alleviated the 24-h blood flow disorder effect caused by Hg, while it was not as significant as f-PET in reducing the toxicity of Hg to zebrafish embryos in the later period of exposure. Thus, the combined toxic effect of microplastics and Hg was related to the shape of microplastics.

Key words： microplastics shape mercury zebrafish embryo joint toxicity

收稿日期: 2022-08-16

PACS:

X171

基金资助:国家自然科学基金资助项目(41807487,42277281);国家级大学生创新创业训练项目(202010060009)

通讯作者: 端正花,副教授,duanzhenghua@mail.nankai.edu.cn E-mail: duanzhenghua@mail.nankai.edu.cn

作者简介: 石岩松(2000-),男,河北乐亭人,天津理工大学本科生,上海师范大学硕士研究生,从事微塑料毒理学研究.shiyscn@outlook.com.

引用本文:

石岩松, 王婧, 崔文杰, 王予頔, 张海红, 端正花. 不同形态微塑料和汞对斑马鱼胚胎发育毒性联合作用研究[J]. 中国环境科学, 2023, 43(4): 1978-1984. SHI Yan-song, WANG Jing, CUI Wen-jie, WANG Yu-di, ZHANG Hai-hong, DUAN Zheng-hua. The combined development toxicity of microplastics with different shapes and mercury on Zebrafish embryos. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1978-1984.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I4/1978

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