Effects of antibiotics on toxicity and halocarbons release of microalgae
YAN Xue-feng1, YANG Bin2, YU Hong1, YANG Gui-peng1, HE Zhen1
1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China; 2. Guangxi Key Laboratory of Marine Environmental Change and Disaster in Beibu Gulf, Beibu Gulf University, Qinzhou 535011, China
Abstract：The toxicity of sulfamethazine (SMZ) and oxytetracycline (OTC) to Skeletonema costatum and the potential effects on the release of volatile halocarbons (VHCs) were investigated. The results showed that 10mg/L SMZ and OTC significantly inhibited the growth of Skeletonema costatum in the middle and later period of the culture process. The maximum inhibition rates of cell density, chlorophyll a concentration and Fv/Fm reached the maximum, which was 90.74%, 91.25%, 83.46%, 87.16%, 87.04% and 87.43%, respectively. The soluble protein content under antibiotic exposure was significantly lower than that in the control group. Catalase activity increased first and then decreased with the increase of antibiotic concentration and the prolongation of exposure time, indicating that long-term and high concentration exposure of the two antibiotics showed obvious toxic effects on microalgae, with the toxic effect of SMZ being higher than that of OTC. Antibiotic exposure induced an increase in intracellular reactive oxygen species (ROS) production. In addition, the addition of antibiotics can promote the release of VHCs in different degrees. It may be that the halogenation reaction of H2O2 catalyzed by haloperoxidase (HPO) produce more volatile halocarbons as antioxidants against oxidative stress to avoid the damage caused by excessive ROS to algal cells. These results indicate that the response of release of the three volatile halocarbons from microalgae to antibiotics was controlled by the biomass of algae and the catalytic mechanism of HPO enzyme.
阎学凤, 杨斌, 于红, 杨桂朋, 何真. 抗生素对微藻的毒性作用和卤代烃释放的影响[J]. 中国环境科学, 2023, 43(4): 1985-1994.
YAN Xue-feng, YANG Bin, YU Hong, YANG Gui-peng, HE Zhen. Effects of antibiotics on toxicity and halocarbons release of microalgae. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1985-1994.
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