基于微生物完整性指数的水生态系统健康评价——以武汉市东西湖区湖泊群为例

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摘要在基于生物完整性指数(IBI)的生态系统健康评价中,通常会采用鱼类、底栖生物、着生藻类作为指示物种.然而,随着生态退化影响下生产者和消费者的数量锐减,可获得用于评价样本的数量越来越少,这就使得上述指示物种作为健康评价指标的可应用性大大降低.以武汉市东西湖区5个湖泊为研究对象,利用环境DNA(eDNA)技术监测的水体微生物数据,建立微生物完整性指数(M-IBI)评价方法.综合水质和实地生境条件对采样点健康状况进行评分和比较,选择甘家教湖的5个采样点作为参考点.结合遴选的6个重要的生物指标,构建了生态系统健康评价打分方法. 研究结果显示在全部19个采样点中,7个健康等级为健康,占36.84%;8个健康等级为亚健康,占42.11%;4个健康等级为一般,占21.05%,甘家教湖和杨泗泾湖所有采样点均为健康水平.评价结果合理地反映了不同湖泊的健康状况,说明基于eDNA的M-IBI方法在受损湖泊水生态系统健康评价中具有广泛的应用潜力.

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关键词 ：生物完整性指数, 环境DNA, 微生物群落, 湖泊生态系统健康, 城市湖泊

Abstract：In the assessment of ecosystem health based on Index of Integrity (IBI), fish, benthic organisms, and living algae are often used as indicators. However, the number of samples available for assessment has become lesser with a sharp decrease in the producers and consumers. Here, five lakes in Dongxihu District of Wuhan City were taken as the research area, and the Microbe IBI (M-IBI) evaluation approach was established by using the water microorganism data obtained with the environmental DNA (eDNA) technology. After comparing and scoring the water quality and field habitat conditions, five sampling points in Ganjiajiao Lake were selected as the reference; and six important biological indicators were selected to develop the ecosystem health scoring and evaluation approach. The results show that among all the 19sampling points, 7sites were evaluated being healthy, accounting for 36.84%; 8sites being sub-healthy, accounting for 42.11%; and 4sites being general, accounting for 21.05%. All the sites in Ganjiajiao Lake and Yangsijing Lake were at the health level. The evaluation results can reasonably reflect the health status of different lakes, indicating a wide application potential of the eDNA-based M-IBI method in the health assessment of degraded lake ecosystems.

Key words： index of biotic integrity environmental DNA microbial community lake ecosystem health urban lakes

收稿日期: 2022-09-15

PACS:

X524

基金资助:湖北省技术创新专项重大项目(2019ACA154)资助

通讯作者: 张鹏,副教授,zhang1230@whu.edu.cn E-mail: zhang1230@whu.edu.cn

作者简介: 张迪涛(2000-)男,安徽芜湖人,武汉大学水利水电学院本科生,主要从事生态水利方向研究.2047432292@qq.com

引用本文:

张迪涛, 张鹏, 王司阳, 张亮, 常剑波. 基于微生物完整性指数的水生态系统健康评价——以武汉市东西湖区湖泊群为例[J]. 中国环境科学, 2023, 43(6): 3055-3067. ZHANG Di-tao, ZHANG Peng, WANG Si-yang, ZHANG Liang, CHANG Jian-bo. Health assessment of aquatic ecosystem based on microbial integrity index——A case study on lakes in Dongxihu District of Wuhan. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 3055-3067.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I6/3055

[1] 刘玉灿,田一,苏庆亮,等.我国地表水污染现状与防治策略探索[J]. 净水技术, 2021,40(11):62-70. Liu Y C, Tian Y, Su Q L, et al. Current situation and control strategy of surface water pollution at home[J]. Water Purification Technology, 2021,40(11):62-70.
[2] 蔡琨,张杰,徐兆安,等.应用底栖动物完整性指数评价太湖生态健康[J]. 湖泊科学, 2014,26(1):74-82. Cai K, Zhang J, Xu Z A, et al. Application of a benthic index of biotic integrity for the ecosystem health assessment of Lake Taihu[J]. Journal of Lake Sciences, 2014,26(1):74-82.
[3] Karr J R. Assessment of biotic integrity using fish communities[J]. Fisheries, 1981,6(6):21-27.
[4] Kerans B L, Karr J R. A benthic index of biotic integrity (B-IBI) for rivers of the Tennessee Valley[J]. Ecological Applications, 1994,4(4):768-785.
[5] Wu Z S, Kong M, Cai Y J, et al. Index of biotic integrity based on phytoplankton and water quality index:Do they have a similar pattern on water quality assessment? A study of rivers in Lake Taihu Basin, China[J]. Science of the Total Environment, 2019,658:395-404.
[6] Hill B H, Herlihy A T, Kaufmann P R, et al. Use of periphyton assemblage data as an index of biotic integrity[J]. Journal of the North American Benthological Society, 2000,19(1):50-67.
[7] Stevenson R J. Diatom indicators of stream and wetland stressors in a risk management framework[J]. Environmental Monitoring and Assessment, 1998,51(1/2):107-118.
[8] 朱迪,常剑波.长江中游浅水湖泊生物完整性时空变化[J]. 生态学报, 2004,24(12):2761-2767. Zhu D, Chang J B. Evaluation on temporal and spatial changes of biological integrity for shallow lakes in the middle reach of the Yangtze River[J]. Acta Ecologica Sinica, 2004,24(12):2761-2767.
[9] 王备新,杨莲芳,刘正文.生物完整性指数与水生态系统健康评价[J]. 生态学杂志, 2006,25(6):707-710. Wang B X, Yang L F, Liu Z W, et al. Index of biological integrity and its application in health assessment of aquatic ecosystem[J]. Chinese Journal of Ecology, 2006,25(6):707-710.
[10] 王备新,杨莲芳,胡本进,等.应用底栖动物完整性指数B-IBI评价溪流健康[J]. 生态学报, 2005,25(6):1481-1490. Wang B X, Yang L F, Hu B J, et al. A preliminary study on the assessment of stream ecosystem health in south of Anhui Province using benthic-index of biotic integrity[J]. Acta Ecologica Sinica, 2005,25(6):1481-1490.
[11] 殷旭旺,张远,渠晓东,等.浑河水系着生藻类的群落结构与生物完整性[J]. 应用生态学报, 2011,22(10):2732-2740. Yin X W, Zhang Y, Qu X D, et al. Community structure and biological integrity of periphyton in Hunhe River water system of Liaoning Province, Northeast China[J]. Chinese Journal of Applied Ecology, 2011,22(10):2732-2740.
[12] Jacob K. Limnology:inland water ecosystems[M]. Upper Saddle River, NJ:Prentice Hall, 2001.
[13] 黄小兰,简正军,叶长盛,等.鄱阳湖河湖交错带枯水期水体细菌群落空间分布[J]. 微生物学通报, 2021,48(6):1862-1873. Huang X L, Jian Z J, Ye C S, et al. Spatial distribution of bacterial community in river-lake ecotone of Poyang lake during dry season[J]. Microbiology China, 2021,48(6):1862-1873.
[14] Zhang F J, Xue B, Yao S C, et al. Organic carbon burial from multi-core records in Hulun Lake, the largest lake in northern China[J]. Quaternary International, 2018,475:80-90.
[15] Martin W H. The microbial diversity of inland waters[J]. Current Opinion in Biotechnology, 2006,17(3):256-261.
[16] Sanket D C, Puspendu B. Simultaneous carbon and nitrogen removal from domestic wastewater using high rate vermifilter[J]. Indian Journal of Microbiology, 2021,61(2):218-228.
[17] Parveen B, Mary I, Vellet A, et al. Temporal dynamics and phylogenetic diversity of free-living and particle-associated Verrucomicrobia communities in relation to environmental variables in a mesotrophic lake[J]. FEMS microbiology ecology, 2013,83(1):189-201.
[18] Kondratieff P, Matthews R, Buikema A. A stressed stream ecosystem:macroinvertebrate community integrity and microbial trophic response[J]. Hydrobiologia, 1984,111(2):81-91.
[19] Daren M C, William H C. Sensitivity and variability of metrics used in biological assessments of running waters[J]. Environmental Toxicology and Chemistry, 1999,18(2):285-291.
[20] 孙晶莹,杨江华,张效伟.环境DNA(eDNA)宏条形码技术对枝角类浮游动物物种鉴定及其生物量监测研究[J]. 生态毒理学报, 2018, 13(5):76-86. Sun J Y, Yang J H, Zhang X W. Identification and biomass monitoring of zooplankton Cladocera species with eDNA Metabarcoding Technology[J]. Asian Journal of Ecotoxicology, 2018,13(5):76-86.
[21] 苏瑶,许育新,安文浩,等.基于微生物生物完整性指数的城市河道生态系统健康评价[J]. 环境科学, 2019,40(3):1270-1279. Su Y, Xu Y X, An W H, et al. Assessment of ecosystem health of an urban river based on the microbe index of biotic integrity (M-IBI)[J]. Environmental Science, 2019,40(3):1270-1279.
[22] 安新丽,陈廷廷,赵晗,等.基于微生物生物完整性指数的地下水生态系统健康评价:以包钢稀土尾矿库周边地下水生态系统为例[J]. 环境科学, 2016,37(9):3413-3422. An X L, Chen T T, Zhao H, et al. Assessment of ecosystem health of Baogang Tailings groundwater based on microbiome index of biotic integrity (M-IBI)[J]. Environmental Science, 2016,37(9):3413-3422.
[23] 朱文婷,钱玮,于淼,等.基于微生物完整性指数的苏州市湿地健康评价[J]. 环境科学研究, 2019,32(3):423-430. Zhu W T, Qian W, Yu M, et al. Health evaluation of wetlands in Suzhou City based on microbiome index of biotic integrity[J]. Research of Environmental Sciences, 2019,32(3):423-430.
[24] Li J, Li Y, Qian B, et al. Development and validation of a bacteria-based index of biotic integrity for assessing the ecological status of urban rivers:A case study of Qinhuai River basin in Nanjing, China[J]. Journal of Environmental Management, 2017,196:161-167.
[25] Li Y, Yang N, Qian B, et al. Development of a bacteria-based index of biotic integrity (Ba-IBI) for assessing ecological health of the Three Gorges Reservoir in different operation periods[J]. The Science of the total environment, 2018,640-641:255-263.
[26] GB3838-2002 地表水环境质量标准[S]. GB3838-2002 Environmental quality standards for surface water[S].
[27] Stephen B W, J. A R, Daniel M D, et al. An estuarine benthic index of biotic integrity (B-IBI) for Chesapeake Bay[J]. Estuaries, 1997,20(1):149-158.
[28] Ganasan V, Hughes R. Application of an index of biological integrity (IBI) to fish assemblages of the rivers Khan and Kshipra (Madhya Pradesh), India[J]. Freshwater Biology, 1998,40(2):367-383.
[29] Oberdorff T, Hughes R M. Modification of an index of biotic integrity based on fish assemblages to characterize rivers of the Seine Basin, France[J]. Hydrobiologia, 1992,228(2):117-130.
[30] Barbour M T, Gerritsen J, Snyder B D, et al. Rapid bioassessment protocols for use in streams and wadeable rivers:periphyton, benthic macroinvertebrates, and fish[M]. Washington, DC:U.S. Environmental Protection Agency, Office of Water, 1999.
[31] Cui W Y, Guo S Y, Meng X Z, et al. Application of adapted benthic index of biotic integrity (B-IBI) for river ecosystem health assessment in Zhanghe River Watershed, China[J]. Polish Journal of Ecology, 2019,66(4):407-415.
[32] 渠晓东,刘志刚,张远.标准化方法筛选参照点构建大型底栖动物生物完整性指数[J]. 生态学报, 2012,32(15):4661-4672. Qu X D, Liu Z G, Zhang Y. Discussion on the standardized method of reference sites selection for establishing the benthic-index of biotic integrity[J]. Acta Ecologica Sinica, 2012,32(15):4661-4672.
[33] 何晨凤.基于M-IBI和P-IBI的湖泊生态系统健康和生物完整性机制研究[D]. (北京)华北电力大学, 2018. He C F. Study on the health and biological integrity mechanism of lake ecosystem based on M-IBI and P-IBI[D]. (Beijing) North China Electric Power University, 2018.
[34] 黄艺,舒中亚.基于浮游细菌生物完整性指数的河流生态系统健康评价-以滇池流域为例[J]. 环境科学, 2013,34(8):3010-3018. Huang Y, Shu Z Y. Bacterioplankton index of biotic integrity:An approach for assessing river ecosystem health in Dianchi watershed[J]. Environmental Science, 2013,34(8):3010-3018.
[35] 卢东琪,张勇,蔡德所,等.基于干扰梯度的钦江流域底栖动物完整性指数候选参数筛选[J]. 环境科学, 2013,34(1):137-144. Lu D Q, Zhang Y, Cai D S, et al. Choice of macroinvertebrate metrics for constructing a benthic-index of biotic integrity based on the disturbance gradients in the Qinjiang River Basin[J]. Environmental Science, 2013,34(1):137-144.
[36] 郜星晨,章群,韩博平,等.基于鱼类生物完整性指数的广东鉴江流域环境质量评估[J]. 湖泊科学, 2015,27(4):679-685. Gao X C, Zhang Q, Han B P, et al. Environmental quality assessment of Jian River Basin (Guangdong) based on fish biotic integrity index[J]. Journal of Lake Sciences, 2015,27(4):679-685.
[37] 熊春晖,张瑞雷,徐玉萍,等.应用底栖动物完整性指数评价上海市河流健康[J]. 湖泊科学, 2015,27(6):1067-1078. Xiong C H, Zhang R L, Xu Y P, et al. Health assessment on rivers in Shanghai City using benthic index of biotic integrity[J]. Journal of Lake Sciences, 2015,27(6):1067-1078.
[38] 廖静秋,黄艺.应用生物完整性指数评价水生态系统健康的研究进展[J]. 应用生态学报, 2013,24(1):295-302. Liao J Q, Huang Y. Research progress on using index of biological integrity to assess aquatic ecosystem health[J]. Chinese Journal of Applied Ecology, 2013,24(1):295-302.
[39] 慕林青,张海萍,赵树旗,等.永定河底栖动物生物完整性指数构建与健康评价[J]. 环境科学研究, 2018,31(4):697-707. Mu LQ, Zhang H P, Zhao S Q, et al. River health assessment of Yongding River based on benthic integrated biotic index[J]. Research of Environmental Sciences, 2018,31(4):697-707.
[40] Blocksom K A, Kurtenbach J P, Klemm D J, et al. Development and evaluation of the Lake Macroinvertebrate Integrity Index (LMII) for New Jersey lakes and reservoirs[J]. Environmental monitoring and assessment, 2002,77(3):311-333.
[41] 崔文彦,孟宪智,张世禄,等.大型底栖动物完整性指数在漳河上游水生态评价中的应用[J]. 生物学杂志, 2019,36(5):73-77. Cui W Y, Meng X Z, Zhang S L, et al. Application of benthic index of biotic integrity for aquatic ecological assessment in the upper reaches of Zhanghe River[J]. Journal of Biology, 2019,36(5):73-77.
[42] 张又,蔡永久,张颖,等.河流大型底栖动物对环境压力的响应:以太湖、巢湖流域为例[J]. 湖泊科学, 2021,33(1):204-217. Zhang Y, Cai Y J, Zhang Y, et al. The response of benthic macroinvertebrate communities to environmental pressures in streams and rivers:A case study of Taihu and Chaohu Basins[J]. Journal of Lake Sciences, 2021,33(1):204-217.
[43] 张宇航,渠晓东,王少明,等.浑河流域底栖动物生物完整性指数构建与健康评价[J]. 长江流域资源与环境, 2020,29(6):1374-1386. Zhang Y H, Qu X D, Wang S M, et al. River health assessment of Hun River Basin based on benthic index of biological integrity[J]. Resources and Environment in the Yangtze Basin, 2020,29(6):1374-1386.
[44] 姜永伟,卢雁,问青春,等.基于大型底栖动物完整性指数的辽河流域水生态健康评价[J]. 环境保护科学, 2020,46(6):103-109. Jiang Y W, Lu Y, Wen Q C, et al. Water ecological health assessment of Liaohe River Basin based on the integrity index of benthic macroinvertebrates[J]. Environmental Protection Science, 2020,46(6):103-109.
[45] 黄彬彬,李光锦,丰茂成,等.基于底栖动物生物完整性指数的赣江干流健康评价[J]. 水资源与水工程学报, 2020,31(5):30-36. Huang B B, Li G J, Feng M C, et al. Health assessment of Ganjiang River mainstream based on B-IBI[J]. Journal of Water Resources & Water Engineering, 2020,31(5):30-36.
[46] 刘昊,黄健,陈建林,等.基于快速生物评价协议(RBPs)的漓江上游区河流生境评价[J]. 井冈山大学学报(自然科学版), 2020,41(6):27-32. Liu H, Huang J, Chen J L, et al. River habitat assessment in upstream Lijiang River Basin based on Rapid Bioassessment Protocols (RBPs)[J]. Journal of Jinggangshan University (Natural Science), 2020,41(6):27-32.
[47] 蔡文倩,朱延忠,林岿璇,等.底栖生物完整性指数的构建及生物基准的确定[J]. 中国环境科学, 2016,36(9):2791-2799. Cai W Q, Zhu Y Z, Lin K X, et al. Development on the benthic index of biological integrity and determination for the biocriteria[J]. China Environmental Science, 2016,36(9):2791-2799.
[48] 黄亮亮,吴志强,蒋科,等.东苕溪鱼类生物完整性评价河流健康体系的构建与应用[J]. 中国环境科学, 2013,33(7):1280-1289. Huang L L, Wu Z Q, Jiang K, et al. Development and application of IBI based on fish to assess the river's health in the East Tiaoxi River[J]. China Environmental Science, 2013,33(7):1280-1289.
[49] 陈凯,陈求稳,于海燕,等.应用生物完整性指数评价我国河流的生态健康[J]. 中国环境科学, 2018,38(4):1589-1600. Chen K, Chen Q W, Yu H Y, et al. Methods and prospects of index of biological integrity used for China river ecological health assessment[J]. China Environmental Science, 2018,38(4):1589-1600.
[50] Zuo Y, Xie W F, Pang Y, et al. Bacterial community composition in the gut content of Lampetra japonica revealed by 16S rRNA gene pyrosequencing[J]. PLOS ONE, 2018,12(12):e0188919.
[51] Newton R J, Jones S E, Helmus M R, et al. Phylogenetic ecology of the freshwater Actinobacteria acI lineage[J]. Applied and environmental microbiology, 2007,73(22):7169-7176.
[52] 杜瑞芳,李靖宇,赵吉.乌梁素海湖滨湿地细菌群落结构多样性[J]. 微生物学报, 2014,54(10):1116-1128. Du R F, Li J Y, Zhao J. Bacterial diversity in littoral wetland of Wuliangsuhai lake[J]. Acta Microbiologica Sinica, 2014,54(10):1116-1128.
[53] 高晨晨,郑兴灿,游佳,等.城市污水脱氮除磷系统的活性污泥菌群结构特征[J]. 中国给水排水, 2015,31(23):37-42. Gao C C, Zhen X C, You J, et al. Structure characteristics of activated sludge microbial communities in nitrogen and phosphorus removal system of municipal wastewater[J]. China Water & Wastewater, 2015, 31(23):37-42.
[54] Anna M B, Monika K R, Maciej G, et al. Experimental warming and precipitation reduction affect the biomass of microbial communities in a Sphagnum peatland[J]. Ecological Indicators, 2020,112:106059.
[55] Kozich J J, Westcott S L, Baxter N T, et al. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform[J]. Applied and environmental microbiology, 2013,79(17):5112-5120.
[56] Ramon R M, Bo T, Hendrik S, et al. The response of the microbial community of marine sediments to organic carbon input under anaerobic conditions[J]. Systematic and Applied Microbiology, 1999, 22(2):237-248.
[57] Ashley E, Hannes E, Steven D A, et al. Fundamentals of microbial community resistance and resilience[J]. Frontiers in Microbiology, 2012,3:417.
[58] 徐正,张利平,孙磊.TGGE法对白洋淀底泥细菌种群多样性及动态变化的研究[J]. 科技信息(学术研究), 2008,(14):304-306. Xu Z, Zhang L P, Sun L. Study on the diversity and dynamic change of bacterial population in Baiyang Lake sediment by TGGE[J]. Science & Technology Information (Academic Research), 2008,(14):304-306.
[59] Tong Y, Lin G F, Ke X, et al. Comparison of microbial community between two shallow freshwater lakes in middle Yangtze basin, East China[J]. Chemosphere, 2005,60(1):85-92.
[60] Haukka K, Kolmonen E, Hyder R, et al. Effect of nutrient loading on bacterioplankton community composition in lake mesocosms[J]. Microbial ecology, 2006,51(2):137-146.
[61] 赵立君,刘云根,王妍,等.典型高原湖滨带底泥细菌群落结构及多样性特征[J]. 微生物学通报, 2020,47(2):401-410. Zhao L J, Liu Y G, Wang Y, et al. Bacterial community structure and diversity of sediments in a typical plateau lakeshore[J]. Microbiology China, 2020,47(2):401-410.
[62] Byron C C, John E H. Synchrony and seasonality in bacterioplankton communities of two temperate rivers[J]. Limnology and Oceanography, 2005,50(6):1718-1729.
[63] Hee J S, Muhammad A M M, Mohammad T I. Diversity of secondary metabolites from marine Bacillus species:chemistry and biological activity[J]. Marine Drugs, 2013,11(8):2846-2872.
[64] Hu A Y, Yang X Y, Chen N W, et al. Response of bacterial communities to environmental changes in a mesoscale subtropical watershed, Southeast China[J]. Science of the Total Environment, 2014,472:746-756.
[65] Liu Y Q, Yao T D, Zhu L P, et al. Bacterial diversity of freshwater alpine Lake Puma Yumco on the Tibetan Plateau[J]. Geomicrobiology Journal, 2009,26(2):131-145.
[66] 李建松,王广军,龚望宝,等.蓝藻水华发生过程中细菌群落结构的动态变化[J]. 中国农业大学学报, 2017,22(7):134-142. Li J S, Wang G J, Gong W B, et al. Dynamic changes of bacterial community structure in the occurrence process of cyanobacterial bloom[J]. Journal of China Agricultural University, 2009,26(2):131-145.
[67] 田川.太湖浮游细菌分子生态学及溶藻细菌的研究[D]. (上海)上海交通大学, 2012. Tian C. Research on bacterioplankton diversity and the algicidal bacteria in Lake Taihu[D]. (Shanghai) Shanghai Jiao Tong University, 2012.
[68] 郑小红,肖琳,任晶,等.玄武湖微囊藻水华暴发及衰退期细菌群落变化分析[J]. 环境科学, 2008,29(10):2956-2962. Zheng X H, Xiao L, Xiao J, et al. Variation of bacterial community composition in the outbreak and decline of mcrocystis spp. bloom in Lake Xuanwu[J]. Environmental Science, 2008,29(10):2956-2962.
[69] Amaral-Zettler L A, Rocca J D, Lamontagne M G, et al. Changes in microbial community structure in the wake of Hurricanes Katrina and Rita[J]. Environmental science & technology, 2008,42(24):9072-9078.
[70] Niño-García J P, Ruiz-González C, Del G P A. Interactions between hydrology and water chemistry shape bacterioplankton biogeography across boreal freshwater networks[J]. The ISME journal, 2016,10(7):1755-1766.
[71] Simonin M, Voss K A, Hassett B A, et al. In search of microbial indicator taxa:shifts in stream bacterial communities along an urbanization gradient[J]. Environmental microbiology, 2019,21(10):3653-3668.
[72] Jacob D H, Jacob D H, Jacob D H, et al. Watershed urbanization linked to differences in stream bacterial community composition[J]. Frontiers in Microbiology, 2017,8:1452.
[73] Short J, Tibby J, Vandergoes M J, et al. Using palaeolimnology to guide rehabilitation of a culturally significant lake in New Zealand[J]. Aquatic Conservation:Marine and Freshwater Ecosystems, 2022,32(6):931-950.