四环素降解对厌氧反硝化产甲烷性能的影响

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摘要为有效去除污水中的四环素(TC),以乙酸钠为共代谢基质,研究厌氧反硝化同时产甲烷(SDM)体系中TC的降解特性及体系性能变化.结果表明,5d内SDM体系中TC (1mg /L)的降解率可达82.6%,同时TC降解过程中TOC的降解率可达95%以上;但一定浓度的TC压力对TC的降解、NO₃^--N去除以及CH₄的产生有抑制作用,影响SDM处理废水的性能.此外,根据中间产物推测出该反应体系中微生物对TC的降解途径主要包括羟基化、碳键的断裂和脱氨基等;通过高通量测序对微生物群落进行分析,发现厌氧功能菌群得到富集,细菌菌门以Proteobacteria为主,菌属以Candidatus_Promineofilum和Candidatus_Microthrix为主;古菌菌门主要以Euryarchaeota和Halobacterota为主,菌属以Methanosaeta和Methanothrix为主.出水生物毒性检测结果显示,周期末出水生物毒性降低且接近空白组.

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	龚勍
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	岳秀萍

关键词 ：反硝化产甲烷, 四环素, 中间产物, 微生物群落, 生物毒性

Abstract：To effectively remove tetracycline (TC) from wastewater, the degradation characteristics and system performance changes of TC under anaerobic denitrification simultaneous methanogenesis (SDM) system were investigated using sodium acetate as a co-metabolizing substrate. The degradation rate of TC (1mg/L) in SDM system could reach 82.6% within 5d, and the degradation rate of TOC during TC degradation could reach more than 95%; however, a certain concentration of TC pressure had an inhibitory effect on TC degradation, NO₃^--N removal and CH₄production, which affected the performance of SDM treatment wastewater. In addition, the degradation pathways of TC by microorganisms in the reaction system were mainly hydroxylation, carbon bond breaking and deamination, etc. Based on the intermediate products, it was concluded that the anaerobic functional groups were enriched by high-throughput sequencing, and the bacterial phylum were Proteobacteria, and the dominant genera were Candidatus_Promineofilum and Candidatus_Microthrix. The Archaebacteria phylum were mainly Euryarchaeota and Halobacterota, and the dominant genera were Methanosaeta and Methanothrix. Finally, the effluent biotoxicity was tested and the results showed that the effluent biotoxicity was reduced at the end of the cycle and was close to that of the blank group.

Key words： denitrification and methanogenesis tetracycline intermediate products microbial community biotoxicity

收稿日期: 2022-11-03

PACS:

X703.5

基金资助:山西省基础研究计划面上项目(202103021224064)

通讯作者: 汪素芳,副教授,wangsufang@tyut.edu.cn E-mail: wangsufang@tyut.edu.cn

作者简介: 龚勍(1998-),女,河南驻马店人,太原理工大学环境科学与工程学院硕士研究生,主要从事难降解和新型污染物定向生物转化方面研究.gongqing1102@163.com

引用本文:

龚勍, 王震, 邢剑波, 汪素芳, 周爱娟, 岳秀萍. 四环素降解对厌氧反硝化产甲烷性能的影响[J]. 中国环境科学, 2023, 43(6): 2899-2907. GONG Qing, WANG Zhen, XING Jian-bo, WANG Su-fang, ZHOU Ai-juan, YUE Xiu-ping. Degradation of tetracycline under denitrification and methanogenesis system and its performance change. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 2899-2907.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I6/2899

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