低温对异养硝化菌<i>Acinetobacter</i> sp.生长代谢及群体感应影响研究

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摘要为进一步探究低温条件下HN-AD菌氮素转化机理及其群体感应系统对低温响应机制,采用GC-MS测定HN-AD过程中释放的信号分子,结果表明菌株JQ1004至少分泌包括C₆-, C₈-,和C₁₀-HSL在内3种高丝氨酸内酯(AHLs),其浓度变化均随细菌浓度增加不断累积,呈现显著密度依赖性.当温度由30℃降低至10℃时, C₆-和C₈-HSL呈不断下降趋势,而C₁₀-HSL变化为先上升后下降,并在20℃达到最大值10.89nmol/L,表明C₁₀-HSL在菌株适应低温环境中发挥重要作用.低温添加10nmol/L C₁₀-HSL可显著缩短细菌迟滞期,提高脱氮速率.20℃和10℃时添加C₁₀-HSL使amoA表达量分别提高了35%和22%, napA表达量分别增加了14%和9.4%. C₁₀-HSL通过提高luxR调控的受体蛋白活性来提高菌株群感能力.低温添加C₁₀-HSL可促进胞外蛋白分泌,降低多糖含量,提高生物膜活性,进而强化其对低温恶劣环境适应能力.

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	王秀杰

关键词 ：异养硝化, 好氧反硝化, 群体感应, 低温, AHLs

Abstract：To further explore the mechanism of nitrogen transformation and quorum sensing system of HN-AD bacteria under low temperature, there were at least three AHLs signaling molecules, including C₆-, C₈-, and C₁₀-HSL produced by the HN-AD bacterium JQ1004 based on the analysis of GC-MS. The concentration of AHLs showed a significant density dependence and continuously accumulated with the increase of biomass. When the temperature decreased from the optimal 30℃ to 10℃, C₆- and C₈-HSL showed a continuous decreasing trend due to the decrease of cell concentration, while the concentration of C₁₀-HSL increased first and then decreased, reaching a maximum value of 10.89nmol/L at 20℃, indicating that C₁₀-HSL played a crucial role in the bacterial adaptation to low-temperature environments. Further study found that adding 10nmol/L C₁₀-HSL at low temperature could significantly shorten the bacterial lag period and improved the nitrogen removal rate. The addition of C₁₀-HSL at 20℃ and 10℃ increased the expression of amoA by 35% and 22%, respectively, while the expression of napA increased by 14% and 9.4%. C₁₀-HSL enhanced the ability of quorum sensing of strains by increasing the receptor protein activity regulated by luxR. Adding C₁₀-HSL at low temperature could promote extracellular protein secretion, reduce polysaccharide content, improve biofilm activity, and strengthen its adaptability to harsh low-temperature environments.

Key words： heterotrophic nitrification aerobic denitrification quorum sensing low temperature AHLs

收稿日期: 2023-11-07

PACS:

X703.5

基金资助:江苏省自然科学基金资助项目(BK20210896)

通讯作者: 王秀杰,讲师,18810925108@163.com E-mail: 18810925108@163.com

作者简介: 王乙舒(2000-),女,江苏张家港人,江苏科技大学硕士研究生,主要从事污水生物脱氮研究.1071358707@qq.com.

引用本文:

王乙舒, 李圆, 侯连刚, 陈慧萱, 侯惠敏, 王秀杰. 低温对异养硝化菌Acinetobacter sp.生长代谢及群体感应影响研究[J]. 中国环境科学, 2024, 44(7): 4063-4070. WANG Yi-shu, LI Yuan, HOU Lian-gang, CHEN Hui-xuan, HOU Hui-min, WANG Xiu-jie. Effects of low temperature on the growth, metabolism, and quorum sensing of heterotrophic nitrifying bacteria Acinetobacter sp. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(7): 4063-4070.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I7/4063

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