硫化物对厌氧氨氧化耦合自养脱硫反硝化工艺脱氮除硫效能的影响

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摘要以成熟Anammox颗粒污泥与产甲烷颗粒污泥作为接种物实现了硫自养反硝化耦合厌氧氨氧化(SADA)工艺快速启动,探究了不同硫化物负荷对SADA工艺脱氮效能的影响及其脱硫机理.结果表明:较高硫化物负荷(>1.50g S/(L·d))对耦合系统中AnAOB无显著抑制作用,相应的系统脱氮效率连续运行1.5个月后趋于稳定.当将硫化物浓度降为零,耦合系统的总氮去除率(TNRE)仍能达到88.1%,相应的化学计量比R_s(1.23 ± 0.13)与R_p(0.33 ± 0.08)亦与Anammox理论值近似,表明解除高硫化物负荷胁迫条件后SADA系统中Anammox颗粒污泥仍能实现生物脱氮过程.当解除高硫化物负荷胁迫1.5个月后,在较低硫化物负荷条件下(0.30g S/(L·d))恢复硫化物胁迫,耦合系统出水NO₃^-降低,相应的R_p降至0.21,表明SADA系统仍能较好地实现同步脱氮除硫,兼具硫自养反硝化和厌氧氨氧化的双重功效.系统存在的产甲烷颗粒污泥降低硫化物对AnAOB抑制,缩短了SADA工艺启动时间,且能在重新引入硫化物后快速激活脱硫反硝化过程.通过颗粒污泥形态分析和高通量测序菌群解析可知:不同硫化物胁迫条件下,SADA系统中仍能实现AnAOB (如Candidatus Kuenenia,16.9%)和硫氧化菌(如Thiobacillus,31.6%)的共存富集,且能实现高硫化物负荷条件下较高的TNRE (> 60%)和硫单质产率(95.2%).

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	王雅歌
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	金仁村
	邢保山

关键词 ：硫化物, 厌氧氨氧化, 硫自养反硝化, 耦合工艺, 产甲烷颗粒污泥, 脱氮除硫

Abstract：In this study, mature anammox granular sludge and methanogenic granular sludge were used as inoculum to achieve rapid start-up of the sulfide-driven autotrophic denitrification and anammox (SADA) process. The effects of different sulfide loading on nitrogen removal of the SADA process and its desulfurization mechanism were investigated. Results showed that high sulfide loads (>1.50g S/(L·d)) had no significant inhibitory effect on Anammox bacteria (AnAOB), and the corresponding nitrogen removal efficiency stabilized after 1.5 months of continuous operation. When the sulfide concentration was reduced to zero, the total nitrogen removal efficiency (TNRE) still reached 88.1%, and the stoichiometric ratios of R_s (1.23 ± 0.13) and R_p (0.33 ± 0.08) were similar to the theoretical values of Anammox reaction, indicating that the AnAOB was capable of performing nitrogen removal after removing the high sulfide loading conditions. When the high sulfide loading stress was relieved for 1.5months and was replaced by lower sulfide loading (0.30g S/(L·d)), the effluent showed a decrease in NO₃^- with the R_p of 0.21, indicating that the SADA system achieved simultaneous nitrogen and sulfur removal. The presence of methanogenic granular sludge reduced the inhibition of AnAOB caused by the sulfide, which shortened the start-up time of the SADA process. Meanwhile, the coupling system can activate the desulphurization-denitrification process quickly after reintroducing sulfide. Morphological analysis and high-throughput sequencing technique showed that in the SADA system AnAOB (e.g., Candidatus Kuenenia, 16.9%) and sulfur-oxidizing bacteria (e.g.Thiobacillus, 31.6%) were enriched under different sulfide stresses, contributing to high total nitrogen removal (>60%) and sulfur production (95.2%) under high sulfide loading.

Key words： sulfide anaerobic ammonia oxidation (Anammox) sulfide-driven autotrophic denitrification coupled process methanogenic granular sludge denitrification and desulfurization

收稿日期: 2024-03-02

PACS:

X703

基金资助:陕西省杰出青年科学基金项目(2022JC-031);国家自然科学基金资助项目(52100162)

通讯作者: 邢保山,副教授,baoshanxing@xauat.edu.cn E-mail: baoshanxing@xauat.edu.cn

作者简介: 王雅歌(2000-),男,陕西西安人,西安建筑科技大学硕士研究生,主要从事废水生物脱氮技术研究.ygwang@xauat.edu.cn.

引用本文:

王雅歌, 唐喜芳, 刘佳怡, 付钰琳, 郭琼, 陈荣, 金仁村, 邢保山. 硫化物对厌氧氨氧化耦合自养脱硫反硝化工艺脱氮除硫效能的影响[J]. 中国环境科学, 2024, 44(9): 4901-4909. WANG Ya-ge, TANG Xi-fang, LIU Jia-yi, FU Yu-lin, GUO Qiong, CHEN Rong, JIN Ren-cun, XING Bao-shan. Influence of sulfide on the desulfurization-denitrification performance of the sulfide-driven autotrophic denitrification and anaerobic ammonia oxidation process. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(9): 4901-4909.

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