Culture and enrichment characteristics of anammox biofilm with anoxic biofilm
JIANG Rui1, LI Ren1, YU Li-fang1,2, LIU Ran1, LIU Tian1, ZHANG Ri-xia3
1. School of Municipal and Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; 2. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an 710055, China; 3. Xi'an Municipal Design and Research Institute Co., Ltd, Xi'an 710068, China
Abstract：The anaerobic ammonium oxidation (Anammox) biofilm was enriched and cultured in an up-flow anaerobic fixed bed biofilm (UAFB) reactor by inoculating the anoxic biofilm of a sewage treatment plant (STP) in Xi'an. The feasibility of this strategy was investigated by measuring the Anammox activity, hydrazine dehydrogenase (HZO) activity, and microbial community succession within the biofilms. The UAFB-Anammox reactor was successfully started up within 29 days, with a total nitrogen (TN) removal rate of 76.22%. When the surface nitrogen loading rate (SNLR) from 0.23g/(m2·d) to 2.59g/(m2·d), the maximum Anammox activity reached 2.15g/(m2·d), the TN removal rate was 83.68%. Illumina MiSeq sequencing results revealed that the AnAOB was significant enriched and domianted by Ca. Brocadia with a relative abundance of 33.38%. Concurrently, the denitrifying bacteria Denitratisoma, which colonized in the anoxic biofilm, appeared to utilize the NO3--N produced by the AnAOB, thereby establishing a symbiotic relationship. These findings suggest that the use of existing anoxic biofilms in STPs can expedite the cultivation and enrichment of Anammox biofilms, providing valuable insights for the application of the Anammox process in the upgrading of STPs.
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