EPD系统对不同类别小分子量PAHs的响应分析

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摘要为了探究内源短程反硝化(EPD)系统对不同种类小分子量多环芳烃(PAHs)的耐受性,同时探索提高EPD系统抗冲击能力的方法,首先采用20mg/LPAHs(菲、蒽)分别驯化EPD系统,再通过批次实验向驯化的EPD系统中加入0~80mg/L其他种类的PAHs(蒽、菲、萘)分析驯化的EPD系统对不同种类PAHs的耐受性机理.结果表明,EPD系统在菲和蒽胁迫下,保持了高达86%的NO₂⁻-N积累率和50%的PO₄^3--P去除能力.蒽系统中的微生物分泌出更多的胞外聚合物来保护自身,而菲系统富集了更多的PAH-RHDGNF/R和PAH-RHD GPF/R基因来提高对PAHs的耐受性.菲和蒽的引入显著富集了反硝化聚糖菌和反硝化聚磷菌.经菲驯化的EPD系统反硝化菌活性为(167.429±2.321)mgN/(gVSS×h),且在萘和蒽的胁迫下仍保持良好的除磷能力,经蒽驯化的EPD系统,在萘和菲的胁迫下保持高NO₂⁻-N积累能力,且反硝化菌活性为(220.137±0.575)mgN/(gVSS×h).本研究为EPD系统对于小分子量PAHs耐受性以及通过技术手段提高系统抗冲击能力提供理论支持,对于优化EPD系统在污水处理中的运行效果具有显著意义.

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	白芷瑄
	闫业宇
	吉建涛
	金宝丹
	刘叶
	杜京京
	王兰

关键词 ：内源短程反硝化, 多环芳烃, 耐受性, 功能基因, 菌群结构, 反硝化菌活性

Abstract：In order to investigate the tolerance of endogenous partial denitrification (EPD) system to different types of low molecular weight polycyclic aromatic hydrocarbons (PAHs) and to explore methods to enhance the impact resistance of EPD systems, this study first acclimated EPD systems with 20mg/L PAHs (phenanthrene and anthracene), and then added other types of PAHs (anthracene, phenanthrene, and naphthalene) at concentrations of 0~80mg/L to the EPD system to analyze the mechanisms of PAHs tolerance by batch tests. The results indicated that under the stress of phenanthrene and anthracene, the EPD systems maintained a high accumulation rate of 86% for NO₂^--N and a removal capacity of 50% for PO₄^3--P. In the anthracene system, the microorganisms secreted more extracellular polymeric substances to protect themselves, while a greater enrichment of PAH-RHD GNF/R and PAH-RHD GPF/R genes was observed to enhance tolerance to PAHs in the phenanthrene system. The introduction of phenanthrene and anthracene significantly enriched denitrifying glycan bacteria and denitrifying phosphorus accumulating bacteria. The denitrifying activity of the EPD system acclimated with phenanthrene was (167.429±2.321)mgN/(gVSS×h), and it still maintained a well phosphorus removal capacity under the stress of naphthalene and anthracene. The EPD system acclimated with anthracene maintained high NO₂^--N accumulation capacity under the stress of naphthalene and phenanthrene, with denitrifying bacterial activity at (220.137±0.575)mgN/(gVSS×h). This study provides the theoretical support for the tolerance of EPD systems to low molecular weight PAHs and also proposes insights into enhancing the impact resistance of EPS system through technological interventions, which has significant importance for optimizing the operational effectiveness of EPD in wastewater treatment.

Key words： endogenous partial denitrification polycyclic aromatic hydrocarbons tolerance functional genes microbial community structure denitrifying bacteria activity

收稿日期: 2024-08-05

PACS:

X703

基金资助:国家自然科学基金资助项目(42007340,42377375)

作者简介: 白芷瑄(2000-),女,河南郑州人,郑州大学硕士研究生,研究方向为污水生物处理技术.baizhixuan0221@gs.zzu.edu.cn.

引用本文:

白芷瑄, 闫业宇, 吉建涛, 金宝丹, 刘叶, 杜京京, 王兰. EPD系统对不同类别小分子量PAHs的响应分析[J]. 中国环境科学, 2025, 45(3): 1241-1250. BAI Zhi-xuan, YAN Ye-yu, JI Jian-tao, JIN Bao-dan, LIU Ye, DU Jing-jing, WANG Lan. Response analysis of endogenous partial denitrification system to different categories of low molecular weight PAHs. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(3): 1241-1250.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I3/1241

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