1. College of Environmental and Biological Engineering, Henan University of Engineering, Zhengzhou 451191, China; 2. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Abstract：In this study, 16S rRNA high-throughput sequencing technology was used to analyze the diversity and richness of the phyllosphere bacterial community, and PICRUSt2 software was used to predict the nitrogen metabolism and other functional genes of the phyllosphere bacteria based on the gene sequencing results, and the denitrification rate of the phyllosphere bacteria was measureed. The results showed that Proteobacteria, Actinobacteria, and Bacteroidetes were the dominant phyla in the phyllosphere bacteria of Photinia plants, and Achromobacter, Burkholderia-Caballeronia-Paraburkholderia, and Hymenobacter were the dominant genera, and the dominant bacteria were the same at different locations in the same region. In addition, it was also found that Achromobacter, Massilia, and Sphingomonas, which have denitrification functionsthere, distributed in the phyllosphere of Photinia. The PICRUSt2prediction showed that the metabolic function pathway is dominant of the 6primary functional layers of phyllosphere bacteria, and the membrane transport function is dominant in the 30 secondary functional layers. Meanwhile, the abundance of denitrification related genes is the highest among nitrogen metabolism functional genes, and the abundance of nitrogen fixation related genes is the lowest. The measured denitrification rate of phyllosphere bacteria is (22.2 ± 1.7)-(33.2 ± 4.7) μg/(g·h).
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