自驱弱电强化措施对厌氧氨氧化人工湿地脱氮性能的影响

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (2994 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要将基于ANAMMOX的复合潜流人工湿地(HCW)改造为自驱弱电强化人工湿地(SECW),考察了SECW在试验阶段的运行性能,探究了稳定运行时装置的微生物特性,另解析并阐释了其中的氮转化途径.结果表明,电活性生物膜的预制及电极的合理布设可使SECW中的电极氨氧化作用和阴极反硝化作用得到同步强化,同时亦可使填料层中厌氧氨氧化菌的丰度及活性得以提高.在此基础上,装置中可形成基于ANAMMOX的多路径耦合脱氮体系,由此可显著改善其脱氮性能.当SECW的电极布设方式为“双阴极-单阳极”形式且其水力负荷(HLR)为0.08m³/(m²·d)时,装置的运行性能较为理想,其COD、TP、TN和NH₄⁺-N去除率分别稳定在(91.39±3.09)%、(93.64±1.15)%、(91.67±2.77)%和(94.34±2.72)%,输出电压和输出功率密度分别为(816.45±8.44)mV和651.96W/m³.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	徐湛禹
	汤翀
	刘俊杰
	张剑波
	朱祺琪
	潘玲阳
	李定心
	平腊梅
	李刚
	王振

关键词 ：人工湿地(CW), 自驱弱电强化, 厌氧氨氧化, 电极氨氧化, 阴极反硝化, 脱氮

Abstract：A self-drive weak electric strengthening constructed wetland (SECW), which was renovated from a hybrid subsurface constructed wetland (HCW) based on ANAMMOX, was established in this study, and its operational performance was thoroughly investigated. Further, the microbial characteristics in the system during the steady operational period were explored, and the nitrogen transformation pathways were also analyzed and elucidated. The results showed that, prefabrication of electroactive biofilms and appropriate layout of the electrodes not only simultaneously enhanced electrode-dependent ammonium oxidation and cathodic denitrification in the SECW, but also significantly increased the abundance and activity of anaerobic ammonium-oxidizing bacteria in its substrate layer. Consequently, a multi-path nitrogen removal system based on ANAMMOX was formed in the device, thereby significantly improving the nitrogen removal performance of the SECW. When the electrode configuration was adopted as “double cathode-single anode” and the hydraulic loading rate (HLR) was 0.08m³/(m²·d), the SECW exhibited optimal performance. Specifically, its COD, TP, TN, and NH₄⁺-N removal rates could reach up to (91.39±3.09)%, (93.64±1.15)%, (91.67±2.77)%, and (94.34±2.72)%, respectively. Additionally, the mean output voltage and power density of the system respectively maintained at (816.45±8.44)mV and 651.96W/m³.

Key words： constructed wetland(CW) self-drive weak electric strengthening ANAMMOX electrode-dependent ammoniumoxidation cathodic denitrification nitrogen removal

收稿日期: 2024-10-08

PACS:

X703

基金资助:国家自然科学基金项目(52370160); 安徽省自然科学基金资助项目(2008085ME162); 安徽省高等学校科学研究资助项目(2022AH040121,2023AH051812); 安徽新华学院校级科研团队资助项目(kytd202202)

作者简介: 徐湛禹(2000-),男,安徽合肥人,安徽农业大学硕士研究生,主要从事污水生物资源化处理与回用技术研究.发表论文2篇.986495702@qq.com.

引用本文:

徐湛禹, 汤翀, 刘俊杰, 张剑波, 朱祺琪, 潘玲阳, 李定心, 平腊梅, 李刚, 王振. 自驱弱电强化措施对厌氧氨氧化人工湿地脱氮性能的影响[J]. 中国环境科学, 2025, 45(5): 2490-2502. XU Zhan-yu, TANG Chong, LIU Jun-jie, ZHANG Jian-bo, ZHU Qi-qi, PAN Ling-yang, LI Ding-xin, PING La-mei, LI Gang, WANG Zhen. Effect of self-drive weak electric strengthening measure on nitrogen removal of a constructed wetland based on ANAMMOX. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(5): 2490-2502.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I5/2490

[1] Wu H, Li A, Zhang H, et al. The potential and sustainable strategy for swine wastewater treatment: Resource recovery [J]. Chemosphere, 2023,336:139235.
[2] Li X, Wu S, Yang C, et al. Microalgal and duckweed based constructed wetlands for swine wastewater treatment: A review [J]. Bioresource Technology, 2020,318:123858.
[3] Zhuang L, Yang T, Zhang J, et al. The configuration, purification effect and mechanism of intensified constructed wetland for wastewater treatment from the aspect of nitrogen removal: A review [J]. Bioresource Technology, 2019,293:122086.
[4] Tang S, Liao Y, Xu Y, et al. Microbial coupling mechanisms of nitrogen removal in constructed wetlands: A review [J]. Bioresource Technology, 2020,314:123759.
[5] Negi D, Verma S, Singh S, et al. Nitrogen removal via anammox process in constructed wetland – A comprehensive review [J]. Chemical Engineering Journal, 2022,437:135434.
[6] Gupta S, Srivastava P, Patil S A, et al. A comprehensive review on emerging constructed wetland coupled microbial fuel cell technology: Potential applications and challenges [J]. Bioresource Technology, 2021,320:124376.
[7] 周钦茂,郑德聪,杨暖,等.微生物电化学法处理氨氮废水研究进展[J]. 应用与环境生物学报, 2022,28(3):779-786. Zhou Q, Zheng D, Yang N, et al. Research advances on microbial electrochemical methods for ammonia-contaminated wastewater treatment [J]. Chinese Journal of Applied and Environmental Biology, 2022,28(3):779-786.
[8] He Z, Kan J, Wang Y, et al. Electricity production coupled to ammonium in a microbial fuel cell [J]. Environmental Science and Technology, 2009,43:3391-3397.
[9] Vilajeliu-Pons A, Koch C, Balaguer M D, et al. Microbial electricity driven anoxic ammonium removal [J]. Water Research, 2018,130:168-175.
[10] 王振,余昕洁,孙一波,等.生物电化学系统中阳极氨氧化作用强化及亚硝酸盐累积特性[J]. 应用与环境生物学报, 2023,29(4):875-882. Wang Z, Yu X, Sun Y, et al. Enhancement of anode-dependent ammonium oxidation and nitrite accumulation characteristics in a bioelectrochemical system [J]. Chinese Journal of Applied and Environmental Biology, 2023,29(4):875-882.
[11] Clauwaert P, Rabaey K, Aelterman P, et al. Biological denitrification in microbial fuel cells [J]. Environmental Science & Technology, 2007,41(9):3354-3360.
[12] Su D, Chen Y. Advanced bioelectrochemical system for nitrogen removal in wastewater [J]. Chemosphere, 2022,292:133206.
[13] Vijay A, Sonawane J M, Chhabra M. Denitrification process in microbial fuel cell: A comprehensive review [J]. Bioresource Technology Reports, 2022,17:100991.
[14] 潘玲阳,武威,高磊,等.拔风管对分流式复合潜流人工湿地脱氮性能的影响[J]. 环境工程学报, 2022,16(11):3750-3762. Pan L, Wu W, Gao L, et al. Effect of air duct on nitrogen removal performance of a hybrid subsurface flow constructed wetland with step-feeding [J]. Chinese Journal of Environmental Engineering, 2022,16(11):3750-3762.
[15] 王振,王宁,高磊,等.基于电极氨氧化的全程自养脱氮工艺的运行性能[J]. 应用与环境生物学报, 2023,29(4):883-890. Wang Z, Wang N, Gao L, et al. Operation performance of a completely autotrophic nitrogen removal process based on electrode dependent ammonium oxidation [J]. Chinese Journal of Applied and Environmental Biology, 2023,29(4):883-890.
[16] 国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002:227-285. General Administration of Environmental Protection of the People’s Republic of China. Standard methods for the examination of water and wastewater [M]. Beijing: China Environmental Science Press, 2002: 227-285.
[17] Zhang K, Yang S Q, Wang W, et al. Bioelectrochemical processes and cellulosic carbon source enhance the autotrophic and heterotrophic denitrification of low C/N ratio wastewater in tidal flow constructed wetland - Microbial fuel cells [J]. Journal of Cleaner Production, 2022, 363:132368.
[18] Yin X, Qiao S, Zhou J, et al. Using three-bio-electrode reactor to enhance the activity of anammox biomass [J]. Bioresource Technology, 2015,196:376-382.
[19] 徐湛禹,陆心怡,汤翀,等.阴极层构型对电极氨氧化人工湿地运行性能的影响[J]. 中国环境科学, 2024,44(12):6775-6786. Xu Z, Lu X, Tang C, et al. Effect of cathode layer configuration on operational performance of a constructed wetland based on electrode-dependent ammonium oxidation [J]. China Environmental Science, 2024,44(12):6775-6786.
[20] Zhi W, Yuan L, Ji G, et al. Enhanced long-term nitrogen removal and its quantitative molecular mechanism in tidal flow constructed wetlands [J]. Environmental Science & Technology, 2015,49:4575-4583.
[21] Huang S, Zhang J, Zhang H, et al. Electric field effect of microbial fuel cells on biological reactions: A review [J]. International Biodeterioration & Biodegradation, 2024,194:105886.
[22] Gao Y, Zhang W, Gao B, et al., Highly efficient removal of nitrogen and phosphorus in an electrolysis-integrated horizontal subsurfaceflow constructed wetland amended with biochar [J]. Water Research, 2018,139:301-310.
[23] Chen Y, He X, Zhang Y, et al. Response of nutrients removal efficiency, enzyme activities and microbial community to current and voltage in a bio-electrical anammox system [J]. Journal of Environmental Management, 2024,354:120322.
[24] Yin X, Qiao S, Zhou J, et al. Using three-bio-electrode reactor to enhance the activity of anammox biomass [J]. Bioresource Technology, 2015,196:376-382.
[25] Yin X, Qiao S, Zhou J. Using electric field to enhance the activity of anammox bacteria [J]. Applied Microbiology and Biotechnology, 2015,99:6921-6930.
[26] Li L, Bian D, Wang Q, et al. Performance of anammox enchanced by pulsed electric fields under added organic carbon sources using integrated network and metagenomics analyses [J]. Bioresource Technology, 2023,380:129116.
[27] Li D, Teng L, Guo K, et al. Achieving stable partial nitrification by exploiting lag phase of NOB recovery for selective washout [J]. Environmental Research, 2025,268:120762.
[28] Zhou Y, Wang C, Xu X, et al. Deciphering the partial denitrification function of companion bacteria in mixotrophic anammox systems under different carbon/nitrogen ratios [J]. Journal of Environmental Chemical Engineering, 2023,11(6):111232.
[29] 韩文杰,吴迪,周家中,等.CANON生物膜载体储存及活性恢复研究[J]. 中国环境科学, 2020,40(5):2062-2072. Han W, Wu D, Zhou J, et al. Research on the storage and activity recovery of CANON suspended carrier biofilm [J]. China Environmental Science, 2020,40(5):2062-2072.
[30] Qu B, Fan B, Zhu S, et al. Anaerobic ammonium oxidation with an anode as the electron acceptor [J]. Environmental Microbiology Reports, 2014,6:100-105.
[31] Francis C A, Beman J M, Kuypers M M M. New processes and players in the nitrogen cycle: the microbial ecology of anaerobic and archaeal ammonia oxidation [J]. ISME Journal, 2007,1:19-27.
[32] 李建,占国强,王娟,等.生物电解池氨氧化脱氮产能[J]. 应用与环境生物学报, 2014,20(6):1058-1062. Li J, Zhan G Q, Wang J, et al. Simultaneous production of energy from ammoxidation in microbial electrolysis cells [J]. Chinese Journal of Applied and Environmental Biology, 2014,20(6):1058-1062.
[33] Liu Y, Liu W, Li Y, et al. Layered inoculation of anaerobic digestion and anammox granular sludges for fast start-up of an anammox reactor [J]. Bioresource Technology, 2021,339:125573.
[34] Du R, Peng Y Z, Cao S B, et al. Mechanisms and microbial structure of partial denitrification with high nitrite accumulation [J]. Applied Microbiology and Biotechnology, 2016,100(4):2011-2021.
[35] Tang X, Huang Y, Tan S, et al. Vertical spatial denitrification performance and microbial community composition in denitrification biofilters coupled with water electrolysis [J]. RSC Advances, 2024, 14(22):15431-15440.
[36] Wang L M, Zhou Y, Peng F Q. et al. Intensified nitrogen removal in the tidal flow constructed wetland - microbial fuel cell: Insight into evaluation of denitrifying genes [J]. Journal of Cleaner Production, 2020,264:121580.
[37] Ortega-Martínez E, Toledo-Alarcón J, Fernández E, et al. A review of autotrophic denitrification for groundwater remediation: A special focus on bioelectrochemical reactors [J]. Journal of Environmental Chemical Engineering, 2024,12(1):111552.
[38] Zhao T, Xie B Z, Yi Y, et al. Two polarity reversal modes lead to different nitrate reduction pathways in bioelectrochemical systems [J]. Science of the Total Environment, 2023,856:159185.
[39] Sun G, Austin D. Completely autotrophic nitrogen-removal over nitrite in lab-scale constructed wetlands: evidence from a mass balance study [J]. Chemosphere, 2007,68(6):1120-1128.