Accumulation characteristics of Cd, As, and Pb in cherry radish (Raphanus sativus L.var.radculus pers) grown in composite polluted soil and its safe production thresholds
QI Hao, QIAN Xiang-yu, WAN Ya-nan, LI Hua-fen, WANG Qi, ZHUANG Zhong
Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resource and EnvironmentalSciences, China Agricultural University, Beijing 100193, China
Abstract:In this study, a pot experiment was conducted to simulate soil contaminated with varying levels of cadmium (Cd), arsenic (As), and lead (Pb) to investigate the changes in biomass, the absorption and accumulation of mineral nutrients, as well as the accumulation characteristics of heavy metals under different contamination conditions. Furthermore, soil threshold for Cd, As, and Pb in acidic soil were derived to ensure the safe production of cherry radish. The results indicated that cherry radish exhibited significant toxic effects when heavy metals content added in soil reached the risk control threshold. The contents of iron (Fe), copper (Cu), and zinc (Zn) in root of cherry radish increased initially and then decreased as the level of heavy metals contamination increased, while manganese (Mn) content continuously increased. The root of cherry radish had the highest enrichment and translocation ability for Cd, with average bioconcentration factors (BCF) being 18.6 and 115 times higher than those for As and Pb, respectively. The average translocation factors (TF) for Cd were 4.02 and 2.41 times higher than those for Pb and As, respectively. Based on the National Food Safety Standard (GB2762—2022), and considering the safety of both the roots and shoots of cherry radishes, the derived soil threshold values for safe cherry radish production in acidic soil were 0.30mg/kg for Cd, 171.1mg/kg for As, and 27.5mg/kg for Pb.
祁浩, 钱相宇, 万亚男, 李花粉, 王琪, 庄重. 复合污染土壤中樱桃萝卜对镉、砷和铅的富集特征及其安全生产阈值[J]. 中国环境科学, 2025, 45(5): 2598-2607.
QI Hao, QIAN Xiang-yu, WAN Ya-nan, LI Hua-fen, WANG Qi, ZHUANG Zhong. Accumulation characteristics of Cd, As, and Pb in cherry radish (Raphanus sativus L.var.radculus pers) grown in composite polluted soil and its safe production thresholds. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(5): 2598-2607.
[1] Liang H, Wu W, Zhang Y, et al. Levels, temporal trend and health risk assessment of five heavy metals in fresh vegetables marketed in Guangdong Province of China during 2014~2017[J]. Food Control. 2018,92:107-120. [2] Hou D, O Connor D, Igalavithana A D, et al. Metal contamination and bioremediation of agricultural soils for food safety and sustainability [J]. Nature Reviews Earth & Environment. 2020,1(7):366-381. [3] Ma Y, Wang Y, Chen Q, et al. Assessment of heavy metal pollution and the effect on bacterial community in acidic and neutral soils [J]. Ecological Indicators. 2020,117:106626. [4] Yang L, Huang B, Mao M, et al. Trace metal accumulation in soil and their phytoavailability as affected by greenhouse types in north China [J]. Environmental Science and Pollution Research. 2015,22(9):6679- 6686. [5] Tian K, Huang B, Xing Z, et al. Geochemical baseline establishment and ecological risk evaluation of heavy metals in greenhouse soils from Dongtai, China [J]. Ecological Indicators. 2017,72:510-520. [6] Kianpoor Kalkhajeh Y, Huang B, Hu W, et al. Environmental soil quality and vegetable safety under current greenhouse vegetable production management in China [J]. Agriculture, Ecosystems & Environment. 2021,307:107230. [7] Chen Z, Muhammad I, Zhang Y, et al. Transfer of heavy metals in fruits and vegetables grown in greenhouse cultivation systems and their health risks in Northwest China [J]. Science of the Total Environment. 2021,766:142663. [8] Vejvodová K, Ash C, Dajčl J, et al. Assessment of potential exposure to As, Cd, Pb and Zn in vegetable garden soils and vegetables in a mining region [J]. Scientific Reports, 2022,12(1):13495. [9] Hu W, Zhang Y, Huang B, et al. Soil environmental quality in greenhouse vegetable production systems in eastern China: Current status and management strategies [J]. Chemosphere, 2017,170:183- 195. [10] Wang B, Gao F, Qin N, et al. A comprehensive analysis on sourcedistribution-bioaccumulation-exposure risk of metal(loid)s in various vegetables in peri-urban areas of Shenzhen, China [J]. Environmental Pollution. 2022,293:118613. [11] 祁浩,庄坚,庄重,等.不同种类蔬菜重金属富集特征及健康风险[J]. 环境科学, 2023,44(6):3600-3608. Qi H, Zhuang J, Zhuang Z, et al. Enrichment characteristics of heavy metals and health risk in different vegetables [J]. Environmental Science, 2023,44(6):3600-3608. [12] 杜新民.氮锌肥配施对樱桃萝卜产量和品质的影响[J]. 农业与技术, 2009,29(6):69-72. Du X M. Effect of combined nitrogen and zinc fertilization on yield and quality of cherry radish [J]. Agriculture & Technology, 2009,29(6):69-72. [13] 李海民,叶枢华,王晋娟,等.生物炭基猪粪堆肥对土壤-樱桃萝卜系统的影响及其生态安全风险评价[J]. 环境污染与防治, 2023,45(8): 1138-1143. Li X M, Ye S H, Wang J J, et al., Effects of application with biocharbased pig manure compost on soil - cherry radish system and ecological risk assessment [J]. Environmental Pollution & Control, 2023,45(8):1138-1143. [14] 顾燕青,顾优丽,白倩,等.杭州市菜地蔬菜对土壤重金属的富集特性研究[J]. 农业资源与环境学报, 2015,(4):401-410. Gu Y Q, Gu Y L, Bai Q. Heavy metals accumulation characteristics of vegetables in Hangzhou city, China [J]. Journal of Agricultural Resources and Environment, 2015,32(4):401-410. [15] 吴琦,杨菲,季辉,等.土壤重金属Pb和Cd在小萝卜中的富集特征及产地环境安全临界值[J]. 江苏农业科学, 2010,(4):322-325. Wu Q, Yang F, Ji H, et al. Accumulation characteristics of soil heavy metals Pb and Cd in radish and environmental safety thresholds of production areas [J]. Jiangsu Agricultural Sciences, 2010,(4):322-325. [16] GB 15618—2018土壤环境质量农用地土壤污染风险管控标准(试行) [S]. GB 15618—2018 Soil environmental quality. Risk control standard for soil contamination of agricultural land (on trial) [S]. [17] 张好,董春雨,杨海婵,等.昭通市农田土壤和蔬菜重金属污染评价及相关性分析[J]. 环境科学, 2024,45(2):1090-1097. Zhang H, Dong C Y, Yang H C, et al. Analysis of heavy metal pollution evaluation and correlation of farmland soil and vegetables in Zhaotong City [J]. Environmental Science, 2024,45(2):1090-1097. [18] Chatterjee J, Chatterjee C. Phytotoxicity of cobalt, chromium and copper in cauliflower [J]. Environmental Pollution, 2000,109(1):69- 74. [19] öncel I, Keles Y, üstün A S. Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings [J]. Environmental Pollution, 2000,107(3):315-320. [20] Chibuike G U, Obiora S C. Heavy metal polluted soils: Effect on plants and bioremediation methods [J]. Applied and Environmental Soil Science, 2014,2014:1-12. [21] 杨世勇,王方,谢建春.重金属对植物的毒害及植物的耐性机制[J]. 安徽师范大学学报(自然科学版), 2004,27(1):71-74,90. Yang S Y, Wang F, Xie J C. Toxicity of heavy metals to plants and plant tolerance mechanisms [J]. Journal of Anhui Normal University (Natural Science). 2004,27(1):71-74,90. [22] 张然然,张鹏,都韶婷.镉毒害下植物氧化胁迫发生及其信号调控机制的研究进展[J]. 应用生态学报, 2016,27(3):981-992. Zhang R R, Zhang P, Du S T. Oxidative stress-related signals and their regulation under Cd stress: A review [J]. Chinese Journal of Applied Ecology. 2016,27(3):981-992. [23] 潘攀,刘贝贝,吴琳,等.香蕉对砷镉铅的富集转运特征及土壤重金属安全阈值[J]. 热带作物学报, 2021,42(1):267-274. Pan P, Liu B B, Wu L, et al. Accumulation and transformation of arsenic, cadmium and lead in banana (Musa spp.) and their safety thresholds in soil [J]. 热带作物学报, 2021,42(1):267-274. [24] 王丽香,陈虎,郭峰,等.镉胁迫对花生生长和矿质元素吸收的影响[J]. 农业环境科学学报, 2013,32(6):1106-1110. Wang L X, Chen H, Guo F, et al. Effects of cadmium on peanut growth and mineral nutrient uptake. Journal of Agro-Environment Science. 2013,32(6):1106-1110. [25] 张丽娜,宗良纲,任偲,等.硅对低镉污染水平下水稻幼苗生长及吸收镉的影响[J]. 农业环境科学学报, 2007,26(2):494-499. Zhang L N, Zong L G, Ren C, et al. Effects of Si on rice seedling growth and uptake of Cd in the low level of Cd pollution. Journal of Agro-Environment Science, 2007,26(2):494-499. [26] Rahman A, Hossain M S, Mahmud J, et al. Manganese-induced salt stress tolerance in rice seedlings: regulation of ion homeostasis, antioxidant defense and glyoxalase systems [J]. Physiology and Molecular Biology of Plants, 2016,22(3):291-306. [27] Ramos I, Esteban E, Lucena J J, et al. Cadmium uptake and subcellular distribution in plants of Lactuca sp. Cd–Mn interaction [J]. Plant Science (Limerick), 2002,162(5):761-767. [28] 赵国华,陈炳禄,张云霓. Cd对蔬菜生物量和主要矿质元素吸收量的影响[J]. 土壤通报, 2014,45(3):722-727. Zhao G H, Chen B L, Zhang Y N. Effects of Cd on the biomass and mineral element absorption of four vegetables [J]. Chinese Journal of Soil Science. 2014,45(3):722-727. [29] 宋正国,徐明岗,李菊梅,等.钙对土壤镉有效性的影响及其机理[J]. 应用生态学报, 2009,20(7):1705-1710. Song Z G, Xu M G, Li J M, et al. Effects of calcium on cadmium bioavailability in lateritic red soil and related mechanisms [J]. Chinese Journal of Applied Ecology, 2009,20(7):1705-1710. [30] 董克虞,陈家梅,邓小莹.农作物对镉的吸收累积规律[J]. 环境科学, 1981,(3):6-11. Dong K Y, Chen J M, Deng X Y. Absorption and accumulation patterns of cadmium in crops [J]. Environmental Science, 1981, (3):6-11. [31] 费新东,郝国辉.萝卜、青菜对土壤中重金属镉的累积试验初报[J]. 南方农业, 2020,14(36):143-144. Fei X D, Hao G H. Preliminary report on the accumulation of heavy metal cadmium in radish and choy sum from soil [J]. South China Agriculture, 2020,14(36):143-144. [32] Chang C Y, Yu H Y, Chen J J, et al. Accumulation of heavy metals in leaf vegetables from agricultural soils and associated potential health risks in the Pearl River Delta, South China [J]. Environmental Monitoring and Assessment, 2014,186(3):1547-1560. [33] Kim Y Y, Yang Y Y, Lee Y. Pb and Cd uptake in rice roots [J]. Physiologia Plantarum, 2002,116(3):368-372. [34] Chen X, Ouyang Y, Fan Y, et al. The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root [J]. Journal of Experimental Botany, 2018,69(21):5279-5291. [35] Costa G, Morel J L. Cadmium uptake by Lupinus albus (L.): Cadmium excretion, a possible mechanism of cadmium tolerance [J]. Journal of Plant Nutrition, 1993,16(10):1921-1929. [36] 周启星.复合污染生态学[M]. 北京:中国环境科学出版社, 1995. Zhou Q X. Combined-pollution ecology [M]. Beijing: China Environmental Science Press, 1995. [37] 梁晶,徐仁扣,赵安珍,等.砷酸根促进红壤吸附Cd( ) Ⅱ 的机制[J]. 环境化学, 2007,(4):483-486. Liang J, Xu R K, Zhao A Z, et al. Effect of arsenate on the mechanism of cadmium sorption onto a red soil [J]. Environmental Chemistry, 2007,(4):483-486. [38] 赵迪.小麦镉砷交互作用的研究[D]. 北京:中国农业大学, 2004. Zhao D. The study of cadmium-arsenic interaction in wheat [D]. Beijing: China Agricultural University, 2004. [39] 胡莹,段桂兰,刘云霞,等.砷-铅交互作用对水稻根表铁膜富集及根系吸收砷铅的影响[J]. 环境化学, 2012,31(12):1968-1973. Hu Y, Duan G L, Liu Y X, et al. Effects of arsenic and lead interaction on arsenic and lead accumulation in iron plaque and uptake by rice roots. [J]. Environmental Chemistry, 2012,31(12):1968-1973.