Characteristics of signal molecules in the formation of aerobic granular sludge
WANG Yu-ying1, ZHI Li-ling1, MA Xin-xin1, WANG Shuo1,2, LI Ji1,2
1. Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, China;
2. Collaborative Innovation Center of Water Treatment Technology and Material of Jiangsu Colleges, Jiangnan University, Suzhou 215009, China
The correlation analysis based on the physical and chemical properties of sludge, the removal efficiency of pollutants, the variation of extracellular polymeric substances (EPS) and signal molecules during the granulation process were carried out. It was notable that the contaminants removal efficiency was significantly improved. Compared with activated sludge, the removal rates of COD, NH4+-N and PO43--P increased by factors of 20%, 36% and 57% in mature aerobic granular sludge. The extracellular protein (PN) and polysaccharide (PS) content increased by 116 and 31mg/gMLVSS, respectively. On the basis of laser confocal scanning microscopy (CLSM) analysis, it was remarkable that the protein from EPS increased significantly, which indicated that the protein may play an important role in the granulation process. The activity of phosphodiesterase showed an upward trend, and the second messenger cyclic diguanylic (c-di-GMP) content increased during formation and decreased when AGS became mature, which was consistent with the variation of protein and polysaccharide in EPS. Moreover, according to the SPSS analysis, c-di-GMP has a significantly positive correlation with proteins in Tightly Bound-EPS (TB-EPS). It was speculated that c-di-GMP may accelerate the formation of aerobic granular sludge by regulating the biosynthesis of proteins in TB-EPS.
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WANG Yu-ying, ZHI Li-ling, MA Xin-xin, WANG Shuo, LI Ji. Characteristics of signal molecules in the formation of aerobic granular sludge. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(4): 1516-1524.
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