1. State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;
2. Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China
The effects on the reduction efficiency and microbial community in simultaneous removal of Cr(Ⅵ) and NO3- from water by hydrogen autotrophic reducing bacteria (HARB) using sequential batch experiments was investigated under different Cr (Ⅵ) concentration, NO3- concentration, pH value and H2 content conditions. Results showed that Cr(Ⅵ) can be reduced by active HARB. When the influent concentration of Cr(Ⅵ) was less than 2000μg/L, the reduction rates of Cr(Ⅵ) and NO3-, and the activity of HARB was not affected by the initial concentration. As a preferred electron acceptor, NO3- competed with Cr(Ⅵ), causing a decrease in the reduction rate of Cr(Ⅵ). The optimum pH value for simultaneous reduction of Cr(Ⅵ) and NO3- by HARB was about 7.0. Both acidic and alkaline environments can inhibit the reduction of Cr(Ⅵ). NO2- accumulated gradually with the increase of pH value. H2 as an electron donor was a necessary factor for the reduction of Cr(Ⅵ) and NO3-, while excess H2 did not increase the reduction rate of Cr(Ⅵ) and NO3-.
Xia S, Liang J, Xu X, et al. Simultaneous removal of selected oxidized contaminants in groundwater using a continuously stirred hydrogen-based membrane biofilm reactor[J]. Journal of Environmental Sciences, 2013,25(1):96-104.
[2]
Li H, Lin H, Xu X, et al. Simultaneous bioreduction of multiple oxidized contaminants using a membrane biofilm reactor[J]. Water Environment Research, 2017,89(2):178-185.
[3]
Barnhart J. Joel Barnhart. Occurrences, uses, and properties of chromium[J]. Regulatory Toxicology and Pharmacology, 1997,26(1):S3-S7.
[4]
Deleo P C, Ehrlich H L. Reduction of hexavalent chromium by pseudomonas-fluorescens lb300in batch and continuous cultures[J]. Applied Microbiology and Biotechnology, 1994,40(5):756-759.
[5]
中华人民共和国生态环境部.2018中国生态环境状况公报[M]. 北京:中国环境科学出版社, 2018. Ministry of Ecology and Environment of the People's Republic of China. China's Eco-Environment Status Bulletin in 2018[M]. Beijing:China Environmental Science Press, 2018.
[6]
Wilkin R T, Acree S D, Ross R R, et al. Fifteen-year assessment of a permeable reactive barrier for treatment of chromate and trichloroethylene in groundwater[J]. Science of The Total Environment, 2014,468-469:186-194.
[7]
曹泉,王兴润.铬渣污染场地污染状况研究与修复技术分析[J]. 环境工程学报, 2009,3(8):1493-1497. Cao Q, Wang X. Study on pollution characteristics of contaminated sites with chrome and discussion on common remediation technologies[J]. Chinese Journal of Environmental Engineering, 2009,3(8):1493-1497.
[8]
宫志强,陈坚,杨鑫鑫,等.某铬污染场地地下水抽水方案优化[J]. 环境工程, 2019,37(5):1-3+75. Gong Z, Chen J, Yang X, et al. Optimization of groundwater pumping scheme in a chromium-contaminated site[J]. Environmental Engineering, 2019,37(5):1-3+75.
[9]
Chung J, Nerenberg R, Rittmann B E. Bio-reduction of soluble chromate using a hydrogen-based membrane biofilm reactor[J]. Water Research, 2006,40(8):0-1642.
[10]
Li Z, Xu Sa, Xiao G, et al. Removal of hexavalent chromium from groundwater using sodium alginate dispersed nano zero-valent iron[J]. Journal of Environmental Management, 2019,244:33-39.
[11]
Kapoor A, Viraraghavan T. Nitrate removal from drinking water-Review-Closure[J]. Journal of Environmental Engineering-Asce, 1998,124(9):903.
[12]
Della Rocca C, Belgiorno V, Meric S. Heterotrophic/autotrophic denitrification (HAD) of drinking water:prospective use for permeable reactive barrier[J]. Desalination, 2007,210(1-3):194-204.
[13]
Liu H, Jiang W, Wan D, et al. Study of a combined heterotrophic and sulfur autotrophic denitrification technology for removal of nitrate in water[J]. Journal of Hazardous Materials, 2009,169(1-3):23-28.
[14]
Rittmann B E. Biofilm technology used to improve water quality[J]. Journal of Shanghai Normal University (Natural Science), 2004, 33(4):1-8.
[15]
Martin K J, Nerenberg R. The membrane biofilm reactor (MBfR) for water and wastewater treatment:Principles, applications, and recent developments[J]. Bioresource Technology, 2012,122:83-94.
[16]
Nerenberg R, Rittmann B E. Hydrogen-based, hollow-fiber membrane biofilm reactor for reduction of perchlorate and other oxidized contaminants[J]. Water Science and Technology, 2004,49(11/12):223-230.
[17]
Xia S, Zhong F, Zhang Y, et al. Bio-reduction of nitrate from groundwater using a hydrogen-based membrane biofilm reactor[J]. Journal of Environmental Sciences, 2010,22(2):257-262.
[18]
Tang Y, Zhou C, Van Ginkel S W, et al. Hydrogen permeability of the hollow fibers used in H2-based membrane biofilm reactors[J]. Journal of Membrane Science, 2012,407:176-183.
[19]
杨潇潇.利用CO2作为碳源的氢基质生物膜反应器去处理工业废水中的氧化态污染物[D]. 上海:同济大学, 2017. Yang X X. Bioreduction of oxidized contaminants from industrial wastewater using hydrogen/carbon dioxide membrane aeration biofilm reactor[D]. Shanghai:Tongji University, 2017.
[20]
Xia S, Xu X, Zhou C, et al. Direct delivery of CO2 into a hydrogen-based membrane biofilm reactor and model development[J]. Chemical Engineering Journal, 2016,290:154-160.
[21]
王晨辉,徐晓茵,夏四清.氢基质生物膜反应器去除水中硒酸盐研究[J]. 中国环境科学, 2014,34(6):1442-1447. Wang C, Xu X, Xia S. Removal of selenate from aqueous solution by hydrogen-based membrane biofilm reactor[J]. China Environmental Science, 2014,34(6):1442-1447.
[22]
赵欣欣.基于亚硝酸盐还原酶酶学特性的优势菌除Cr(Ⅵ)机理研究[D]. 广州:广东工业大学, 2013. Zhao X X. Mechanism study on Cr(Ⅵ) removal by a strain of Bacillus sp. based on characteristics of NiR enzyme[D]. Guangzhou:Guangdong University of Technology, 2013.
[23]
余萱.假单胞菌LZ-4同时还原硝酸盐和六价铬的机理及其应用研究[D]. 北京:中国矿业大学, 2017. Yu X. Mechanism and application studies on simultaneous reduction of nitrate and hexavalent chromium by pseudomonas sp. LZ-4[D]. Beijing:China University of Mining and Technology, 2017.
[24]
夏四清,钟佛华,张彦浩.氢自养反硝化去除水中硝酸盐的影响因素研究[J]. 中国给水排水, 2008,24(21):5-8. Xia S, Zhong F, Zhang Y. Study on affecting factors of nitrate removal from water by hydrogen autotrophic denitrification[J]. China Water & Wastewater, 2008,24(21):5-8.
[25]
Knight V, Blakemore R. Reduction of diverse electron acceptors by Aeromonas hydrophila[J]. Archives of Microbiology, 1998,169(3):239-248.
[26]
Liu T, Zhang W, Li X, et al. Kinetics of competitive reduction of nitrate and iron oxides by HS01[J]. Soil Science Society of America Journal, 2014,78(6):1903.
[27]
Harbi B, Chédia Jabeur, Ellafi A, et al. Phenotypic variation and morphological changes in starved denitrifying Aeromonas hydrophila[J]. Annals of Microbiology, 2013,63(3):1039-1045.
[28]
Jinshu L, Taylor J C, Baldwin S A. Removal of selenate from brine using anaerobic bacteria and zero valent iron[J]. Journal of Environmental Management, 2018,222:348-358.
[29]
Martins M, Sandra S, Pereira Inês A C. Anaerobic biodegradation of pharmaceutical compounds:new insights into the pharmaceutical-degrading bacteria[J]. Journal of Hazardous Materials, 2018,357:289-297.
[30]
Zhang J, Guo R B, Qiu Y L, et al. Bioaugmentation with an acetate-type fermentation bacterium acetobacteroides hydrogenigenes improves methane production from corn straw[J]. Bioresource Technology, 2015,179(1):306-313.
[31]
Kwak Y H, Lee D S, Kim H B. Vibrio harveyi nitroreductase is also a chromate reductase[J]. Applied and Environmental Microbiology, 2003,69(8):4390-4395.
[32]
Chardin B, Giudici-Orticoni M T, Luca G, et al. Hydrogenases in sulfate-reducing bacteria function as chromium reductase[J]. Applied Microbiology & Biotechnology, 2003,63(3):315-321.
[33]
Thatoi H, Das S, Mishra J, et al. Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium:A review[J]. Journal of Environmental Management, 2014,146:383-399.
[34]
Alvarez A H, MorenoSánchez R, Cervantes C. Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa[J]. Journal of Bacteriology, 1999,181(23):7398-400.
