盐度对硝酸细菌活性的影响及动力学特性

张宇坤; 张亮; 董怡君; 顾升波; 彭永臻

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中国环境科学 ›› 2020, Vol. 40 ›› Issue (5) : 2047-2052.

水污染与控制

盐度对硝酸细菌活性的影响及动力学特性

张宇坤^1,2,3, 张亮¹, 董怡君¹, 顾升波¹, 彭永臻¹

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Effect of salinity on activity of nitrite-oxidizing bacteria and kinetic characterization

ZHANG Yu-kun^1,2,3, ZHANG Liang¹, DONG Yi-jun¹, GU Sheng-bo¹, PENG Yong-zhen¹

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摘要

为分析盐度对硝酸细菌（NOB）活性的影响及其动力学特性，本文采用高浓度亚硝态氮污水富集培养NOB.对NOB富集污泥进行荧光原位杂交技术（FISH）分析表明Nitrobacter占细菌总数的（81%±6%）.污泥的最大比亚硝态氮氧化速率为（42.5±0.9）mgN/（gVSS·h）.用此污泥考察了盐度对NOB活性的影响，并测定了盐度为10g/L时NOB的动力学参数（Ko、K_S）.结果表明，与盐度为0g/L时的NOB活性相比，盐度为15g/L时NOB活性降低了3.3%；盐度为10和20g/L时的NOB活性分别降低了11%.盐度为10g/L时，NOB的最大比亚硝态氮氧化速率为（37.9±0.7）mgN/（gVSS·h），氧的半饱和常数K_o值为（1.51±0.06）mg/L，底物（亚硝态氮）半饱和常数K_S值为（6.06±0.15）mg/L，Ko、K_S测定值均高于ASM2模型中氨氧化细菌（AOB）推荐值.盐度对NOB的抑制降低了最大比亚硝态氮氧化速率，对氧传递和底物（亚硝态氮）传递均存在影响.

Abstract

In order to analyze the effect of salinity on NOB (nitrite-oxidizing bacteria) activity and kinetic characterization, NOB was enriched in a lab-scale SBR system with high concentration of nitrite wastewater in this study. The FISH (fluorescence in situ hybridization) analysis showed that Nitrobacter accounted for(81%±6%)of total bacteria. The maximum specific nitrite oxidation rate was (42.5±0.9)mgN/(gVSS·h). The effect of salinity on NOB activity was investigated using this mixed culture. The kinetic parameters of NOB (Ko, K_S) were both measured under salinity of 10g/L. Results showed that compared with salinity 0g/L, the activity of NOB decreased 3.3% unders alinity of 15g/L and decreased 11% under salinity of 10, 20g/L. When salinity was 10g/L, the maximum specific nitrite oxidation rate was (37.9±0.7)mgN/(gVSS·h). The half-saturation constant for oxygen (Ko) and nitrite (K_S) were (1.51±0.06), (6.06±0.15) mg/L. The values of Ko and K_S were higher than recommended value of ammonia-oxidizing bacteria (AOB) in ASM2model. It is suggested that salinity could reduce maximum specific nitrite oxidation rate and affect the oxygen affinity and substrate (nitrite) affinity of NOB.

导出引用

张宇坤, 张亮, 董怡君, 顾升波, 彭永臻. 盐度对硝酸细菌活性的影响及动力学特性[J]. 中国环境科学. 2020, 40(5): 2047-2052

ZHANG Yu-kun, ZHANG Liang, DONG Yi-jun, GU Sheng-bo, PENG Yong-zhen. Effect of salinity on activity of nitrite-oxidizing bacteria and kinetic characterization[J]. China Environmental Science. 2020, 40(5): 2047-2052

中图分类号： X703.1

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