Research on the storage and activity recovery of CANON suspended carrier biofilm
HAN Wen-jie1, WU Di1, ZHOU Jia-zhong1, HUANGPU Xiao-liu2, LI Guo2, HE Qiang2, KAN Yu-jiao3
1. Biofilm Research Institute, Qingdao SPRING Water Treatment CO. Ltd., Qingdao 266555, China;
2. Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400044, China;
3. School of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China
The activity recovery effect of CANON-MBBR reactor which had experienced short-term storage at low temperature (20days, 15℃) and low load operation at room temperature (25℃) was studied by using different recovery methods. The results showed that the recovery rate of TN removal load reached 102% by recovering the temperature first and then slowly increasing the influent load. If the influent load was directly raised to the original level without the increase of temperature, the detachment of biofilms of the suspended carriers could happen due to the decrement of EPS content, therefore the recovery rate of TN removal load was lowered to 84%. In addition, the recovery rate of TN removal load reached 96% after 25days of SBR operation under low load and normal temperature (25℃) by the same recovery method. The dominant functional microorganisms in CANON-MBBR system were identified as Nitrosomonas and Candidatus Kuenenia. The relative abundance of both genus could reach over 11% and 27% respectively after the system entered the stable operation by appropriate recovery methods. The microbial composition of biofilms was not significantly influenced by the biofilm detachment.
韩文杰, 吴迪, 周家中, 皇甫小留, 李果, 何强, 阚渝姣. CANON生物膜载体储存及活性恢复研究[J]. 中国环境科学, 2020, 40(5): 2062-2072.
HAN Wen-jie, WU Di, ZHOU Jia-zhong, HUANGPU Xiao-liu, LI Guo, HE Qiang, KAN Yu-jiao. Research on the storage and activity recovery of CANON suspended carrier biofilm. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(5): 2062-2072.
Lackner S, M.Gilbert E, E. Vlaeminck S, et al. Full-scale partial nitritation/anammox experiences-an application survey[J]. Water research, 2014,55:292-303.
[2]
Strous M, Van Gerven E, Zheng P, et al. Ammonium removal from concentrated waste streams with the anaerobic ammonium oxidation (anammox) process in different reactor configurations[J]. Water Research, 1997,31(8):1955-1962.
[3]
Rothrock M J, Vanotti M B, Szögi A A, et al. Long-term preservation of anammox bacteria[J]. Applied microbiology and biotechnology, 2011,92(1):147.
[4]
Vlaeminck S E, Geets J, Vervaeren H, et al. Reactivation of aerobic and anaerobic ammonium oxidizers in OLAND biomass after long-term storage[J]. Applied microbiology and biotechnology, 2007, 74(6):1376-1384.
[5]
李祥,郑宇慧,黄勇,等.保存温度及时间对厌氧氨氧化污泥活性的影响[J]. 中国环境科学, 2011,31(1):56-61. Li X, Zheng Y H, Huang Y, et al. Effect of preservation temperature and time on ANAMMOX sludge activity[J]. China Environmental Science, 2011,31(1):56-61
[6]
袁怡,黄勇,李祥,等.长期保藏对厌氧氨氧化污泥脱氮性能的影响[J]. 环境工程学报, 2014,8(5):2051-2056. Yuan Y, Huang Y, Li X, et al. Influence of long-term preservation on nitrogen removal capabilities of ANAMMOX sludge[J]. Chinese Journal of Environmental Engineering, 2014,8(5):2051-2056.
[7]
Sun H, Peng Y, Shi X. Advanced treatment of landfill leachateusing anaerobic-aerobic process:organic removal bysimultaneous denitritation and methanogenesis and nitrogenremoval via nitrite[J]. Bioresource Technology, 2015,177:337-345.
[8]
Trigo C, Campos J L, Garrido J M, et al. Start-up of the anammox process in a membrane bioreactor[J]. Journal of Biotechnology, 2006, 126(4):475-487.
[9]
米卫星. CANON工艺处理低氨氮废水性能、N2O产生及微生物群落研究[D]. 西安:长安大学, 2018. MI W X. Study on the performance, N2O production and microbial community of CANON process in the treatment of low ammonia nitrogen wastewater[D]. Xi'an:Chang'an University, 2018.
[10]
韩文杰,周家中,吴迪,等.悬浮载体结垢对CANON-MBBR系统影响及恢复控制探究[J]. 化工学报, 2019,70(6):2298-2307. Han W J, Zhou J Z, Wu D, et al. Study on influence of suspended carrier scaling on CANON-MBBR system and restoration control[J]. CIESC Journal, 2019,70(6):2298-2307.
[11]
CJ/T461-2014水处理用高密度聚乙烯悬浮载体填料[S]. CJ/T461-2014 High density polyethylene suspended carrier for water treatment[S].
[12]
孙洪伟,陈翠忠,高宇学,等.碳氮比对活性污泥胞外聚合物的长期影响[J].中国环境科学,2018,38(03):950-958.Sun H W, Chen C Z, Gao Y X, et al. Effect of C/N ratio on extracellular polymeric substance (EPS) in the sequencing batch reactor (SBR)[J]. China Environmental Science,2018,38(03):950-958.
[13]
Edgar R C. Search and clustering orders of magnitude faster than BLAST[J]. Bioinformatics, 2010,26(19):2460-2461.
[14]
Wang Q, Garrity G M, Tiedje J M, et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and environmental microbiology, 2007,73(16):5261-5267.
[15]
Tang R, Wei Y, Li Y, et al. Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy[J]. Gut, 2017:gutjnl-2016-313332.
[16]
汪彩华,郑平,唐崇俭,等.间歇性饥饿对厌氧氨氧化菌混培物保藏特性的影响[J]. 环境科学学报, 2013,33(1):36-43. Wang C H, Zheng P, Tang C J, et al. Effects of intermittent starvation on preservation characteristics of ANAMMOX bacteria[J]. Acta Scientiae Circumstantiae, 2013,33(1):36-43.
[17]
Zhang Z Z, Buayi X, Cheng Y F, et al. Anammox endogenous metabolism during long-term starvation:Impacts of intermittent and persistent modes and phosphates[J]. Separation and Purification Technology, 2015,151:309-317.
[18]
李祥,黄勇,巫川,等.Fe2+和Fe3+对厌氧氨氧化污泥活性的影响[J]. 环境科学, 2014,35(11):4224-4229. Li X, Huang Y, Wu C, et al. Effect of Fe2+ and Fe3+ on the activity of ANAMMOX[J]. Environmental Science, 2014,35(11):4224-4229.
[19]
马金元,左陆珅,李华宇,等.气相条件对ANAMMOX菌种短期保藏效果的影响[J]. 中国环境科学, 2017,37(3):925-931. Ma J Y, Zuo L K, Li H Y, et al. The effect of gas phase conditions on short-term preservation effect of AAMMOX bacteria[J]. China Environmental Science, 2017,37(3):925-931.
[20]
吴迪.MBBR在国内的工程应用与发展前景[J]. 中国给水排水, 2018,(16):22-31. Wu D. Application and development prospect of MBBR in China[J]. China Water & Wastewater, 2018,(16):22-31.
[21]
Zhang L, Zhang S, Peng Y, et al. Nitrogen removal performance and microbial distribution in pilot-and full-scale integrated fixed-biofilm activated sludge reactors based on nitritation-anammox process[J]. Bioresource technology, 2015,196:448-453.
[22]
高雪健,张杰,李冬,等.不同基质浓度对ANAMMOX菌短期储存的影响[J]. 环境科学, 2018,39(12):5587-5595. Gao X J, Zhang J, Li D, et al. Effects of different substrate concentrations on the short-term storage of ANAMMOX bacteria[J]. Environmental Science, 2018,39(12):5587-5595.
[23]
Ma X, Wang Y, Zhou S, et al. Endogenous metabolism of anaerobic ammonium oxidizing bacteria in response to short-term anaerobic and anoxic starvation stress[J]. Chemical Engineering Journal, 2017,313:1233-1241.
[24]
李冬,田海成,梁瑜海,等.水质条件对厌氧氨氧化颗粒污泥EPS含量的影响[J]. 哈尔滨工业大学学报, 2017,49(2):6-12. Li D, Tian H C, Liang Y H, et al. Effect of water quality of influent on the content of extracellular polymeric substances in anammox[J]. Journal of Harbin Institute of Technology, 2017,49(2):6-12.
[25]
杨敏,胡学伟,宁平,等.废水生物处理中胞外聚合物(EPS)的研究进展[J]. 工业水处理, 2011,31(7):7-12. Yang M, Hu X W, Ning P, et al, Research progress in extracellular polymeric substances applied to biological wastewater treatment[J]. Industrial Water Treatment, 2011,31(7):7-12.
[26]
秦玉格.游离氨预处理提高污泥厌氧发酵产酸量[D]. 长沙:湖南大学, 2018. Qin Y G. free ammonia-based pretreatment promotes short-chain fatty acid production from waste activated sludge.[D]. Changsha:Hunan University, 2018.
[27]
帅昆.过氧化钙联合游离氨预处理技术提高污泥厌氧发酵产短链脂肪酸的研究[D]. 长沙:湖南大学, 2018. Shuai K. Enhanced short-chain fatty acids production from waste activated sludge by combining calcium peroxide with free ammonia pretreatment[D]. Changsha:Hunan University, 2018.
[28]
张肖静,李冬,梁瑜海,等.氨氮浓度对CANON工艺性能及微生物特性的影响[J]. 中国环境科学, 2014,34(7):1715-1721. Zhang X J, Li D, Liang Y H, et al. Effect of ammonia concentration on the performance and microbial characteristics of CANON process.[J]. China Environmental Science, 2014,34(7):1715-1721.
[29]
刘竹寒,岳秀,于广平,等.CANON在SBAF中的快速启动及其微生物特征[J]. 环境科学, 2017,38(1):253-259. Liu Z H, Yue X, Yu G P, et al. ast Start-up of SBAF system assisted CANON process and the microbial analysis[J]. Environmental Science, 2017,38(1):253-259.
[30]
李冬,何永平,张肖静,等.MBR系统CANON工艺的快速启动及微生物种群特征[J]. 中国环境科学, 2014,34(11):2788-2795. Li D, He Y P, Zhang X J, et al. The fast start-up of CANON process in MBR system and the characterization of microbes[J]. China Environmental Science, 2014,34(11):2788-2795.
[31]
付昆明,廖敏辉,周厚田,等.降温过程中生物膜CANON反应器的运行特征[J]. 环境科学, 2019,40(3):1412-1418. Fu K M, Liao M H, Zhou H T et al. Operation characteristics of the biofilm CANON reactor during the temperature reduction process[J]. Environmental Science, 2019,40(3):1412-1418.
[32]
Yang Y, Zhang L, Cheng J, et al. Microbial community evolution in partial nitritation/anammox process:From sidestream to mainstream[J]. Bioresource technology, 2018,251:327-333.
[33]
Liang Y, Li D, Zhang X, et al. Nitrogen removal and microbial characteristics in CANON biofilters fed with different ammonia levels[J]. Bioresource technology, 2014,171:168-174.
[34]
Vázquez-Padín J, Mosquera-Corral A, Campos J L, et al. Microbial community distribution and activity dynamics of granular biomass in a CANON reactor[J]. water research, 2010,44(15):4359-4370.
[35]
刘涛,李冬,曾辉平,等.氨氮浓度对CANON工艺功能微生物丰度和群落结构的影响[J]. 环境科学, 2013,34(2):773-780. Liu T, Li D, Zeng H P, et al. Assessment of the effect of influent NH4+-N concentration on the abundance and community structure of functional bacteria in CANON process[J]. Environmental Science, 2013,34(2):773-780.
[36]
Yue X, Yu G, Lu Y, et al. Effect of dissolved oxygen on nitrogen removal and the microbial community of the completely autotrophic nitrogen removal over nitrite process in a submerged aerated biological filter[J]. Bioresource technology, 2018,254:67-74.