三价铁对有机物存在下厌氧氨氧化脱氮的影响

王海月; 彭玲; 毛念佳; 耿金菊; 任洪强; 许柯

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中国环境科学 ›› 2021, Vol. 41 ›› Issue (4) : 1672-1680.

水污染与控制

三价铁对有机物存在下厌氧氨氧化脱氮的影响

王海月, 彭玲, 毛念佳, 耿金菊, 任洪强, 许柯

作者信息 +

Effect of Fe³⁺ on nitrogen removal of Anammox in the presence of organic matter

WANG Hai-yue, PENG Ling, MAO Nian-jia, GENG Jin-ju, REN Hong-qiang, XU Ke

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摘要

考察了三价铁（2.24~7.84mg/L）存在下厌氧氨氧化系统对有机物的耐受性能，并通过16SrRNA高通量测序技术和定量PCR探究其机理.结果表明，进水COD浓度为50和100mg/L时，4个反应器的氨氮和总氮去除率均较高（>90%），三价铁的强化作用不明显；进水COD浓度继续升高（150和200mg/L），厌氧氨氧化受到抑制，三价铁的强化作用逐渐增加；COD浓度为200mg/L时，添加三价铁（7.84mg/L）可将氨氮和总氮去除率由61.3%和79.8%（对照组）提升至71.2%和84.7%.16SrRNA高通量测序技术表明，有机物存在下，污泥微生物群落结构出现变化，主要表现为厌氧氨氧化菌丰度的降低及反硝化菌群的大量增殖，进水添加三价铁可提高浮霉菌（Planctomycetes）的丰度.定量PCR结果表明，三价铁能够提高厌氧氨氧化菌16S rRNA及功能基因hzsB的丰度.

Abstract

The tolerance of Anammox to organic matter in the presence of Fe³⁺ (2.24~7.84mg/L) was investigated by running four reactors (R1control, R2 2.24mg/L Fe³⁺, R3 4.48mg/L Fe³⁺, R4 7.84mg/L Fe³⁺). The mechanism was clarified by using 16SrRNA high-throughput sequencing technology and qPCR. The results showed that high NH₄⁺-N and TN removal rates (> 90%) of all four reactors could be achieved at the influent COD concentration of 50 and 100mg/L, and the presence of Fe³⁺ did not show the obvious positive effect. As the influent COD concentration increased to 150 and 200mg/L, the performance of Anammox was inhibited and the positive effect of Fe³⁺ increased. When the influent COD concentration was 200mg/L, the addition of Fe³⁺ (7.84mg/L) increased the NH₄⁺-N and TN removals from 61.3% and 79.8% (R1) to 71.2% and 84.7%, respectively. 16SrRNA high-throughput sequencing results indicated the decrease of Anammox bacteria and the proliferation of denitrifying bacteria in the presence of organic matter. The presence of Fe³⁺ could increase the abundance of Planctomycetes. Fe³⁺ had promoting effects on the abundance of Anammox 16S rRNA and functional gene hzsB by qPCR data analysis.

导出引用

王海月, 彭玲, 毛念佳, 耿金菊, 任洪强, 许柯. 三价铁对有机物存在下厌氧氨氧化脱氮的影响[J]. 中国环境科学. 2021, 41(4): 1672-1680

WANG Hai-yue, PENG Ling, MAO Nian-jia, GENG Jin-ju, REN Hong-qiang, XU Ke. Effect of Fe³⁺ on nitrogen removal of Anammox in the presence of organic matter[J]. China Environmental Science. 2021, 41(4): 1672-1680

中图分类号： X703

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