Denitrification and anammox processes in sediment of Mihe River, China
LI Jia-lin1,2, QIN Song1,2
1. Laboratory of Coastal Biology and Bioresource Protection, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; 2. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract：Four sites were selected along Mihe River to sample the sediments and characterize physiochemical property to reveal denitrification/anammox processes and their constraints by isotope tracing experiment and molecular biological analysis. The results showed that the rates of denitrification varied from 151.75 to 2847.86μmol/(m2·h), and the anammox rates were in the range of 149.57 to 2109.17μmol/(m2·h). The relative contribution of anammox in the nitrogen removal was in an average of 56.1%. The nirK type was the dominant denitrifier with the abundance of 0.19×106~5.12×106 copies/g in the sediment, which was in the phylum of α- and β- Proteobacteria. The anammox bacteria marked by hzsA gene was in the range of 2.58×102~1.14×104 copies/g, which belonged to the Ca. Brocadia clades in Planctomycetes. Furthermore, the denitrification rate was positively related with TN content and PO43- concentration, while the anammox rate was positively related with TN content and negatively related with sediment density. The physiochemical property of sediment was the main condition determining the nitrogen removal rates. The results highlight that denitrification and anammox were of great significance to reduce nitrogen eutrophication, and sediment environment treatment was a considerable way to regular nitrogen removal rate.
李佳霖, 秦松. 弥河沉积物的反硝化和厌氧氨氧化过程[J]. 中国环境科学, 2021, 41(4): 1588-1596.
LI Jia-lin, QIN Song. Denitrification and anammox processes in sediment of Mihe River, China. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1588-1596.
国家海洋局,2017年中国海洋生态环境状况公报[EB/OL]. http://aoc.ouc.edu.cn/18/9c/c9828a202908/pagem.psp. Academy of Ocean of China, Bulletin on the State of China's Marine Ecology and Environment in 2017[EB/OL]. http://aoc.ouc.edu.cn/18/9c/c9828a202908/pagem.psp.
Diaz R J, Rosenberg R. Spreading dead zones and consequences for marine ecosystems[J]. Science, 2008,321(5891):926-929.
Qu H J, Kroeze C. Past and future trends in nutrients export by rivers to the coastal waters of China[J]. Science of the Total Environment, 2010,408(9):2075-2086.
Kanter D R. Nitrogen pollution:a key building block for addressing climate change[J]. Climate Change, 2018,147(1):11-21.
杨雪琴,连英丽,颜庆云,等.滨海湿地生态系统微生物驱动的氮循环研究进展[J]. 微生物学报, 2018,58(4):633-648. Yang X Q, Lian Y L, Yan Q Y, et al. Microbially-driven nitrogen cycling in coastal ecosystems[J]. Acta Microbiologica Sinica, 2018, 58(4):633-648.
Breemen N V, Boyer E W, Goodale C L, et al. Where did all the nitrogen go? Fate of nitrogen inputs to large watersheds in the northeastern U.S.A.[J]. Biogeochemistry, 2002,57-58(1):267-293.
Wang S, Zhu G, Peng Y, et al. Anammox bacterial abundance, activity, and contribution in riparian sediments of the Pearl River Estuary[J]. Environmental Science and Technology, 2012,46(16):8834-8842.
Zhu G, Wang S, Wang W, et al. Hotspots of anaerobic ammonium oxidation at land-freshwater interfaces[J]. Nature Geoscience, 2013, 6:103-107.
Naeher S, Huguet A, Roose-Amsaleg C L, et al. Molecular and geochemical constraints on anaerobic ammonium oxidation (anammox) in a riparian zone of the Seine Estuary (France)[J]. Biogeochemistry, 2015,123(1):237-250.
Lu X, Bade D L, Leff L G, et al. The relative importance of anammox and denitrification to total N2production in Lake Erie[J]. Journal of Great Lakes Research, 2018,44(3):428-435.
Barnes R T, Smith R L, Aiken G R. Linkages between denitrification and dissolved organic matter quality, Boulder Creek watershed, Colorado[J]. Journal of Geophysical Research, 2012,117:G01014.
Jia Z, Liu T, Xia X, et al. Effect of particle size and composition of suspended sediment on denitrification in river water[J]. Science of the Total Environment, 2016,541:934-940.
Liu W, Yao L, Jiang X, et al. Sediment denitrification in Yangtze lakes is mainly influenced by environmental conditions but not biological communities[J]. Science of the Total Environment, 2018,616-617(3):978-987.
Petersen D G, Blazewicz S J, Firestone M, et al. Abundance of microbial genes associated with nitrogen cycling as indices of biogeochemical process rates across a vegetation gradient in Alaska[J]. Environmental Microbiology, 2012,14(4):993-1008.
Zheng Y, Jiang X, Hou L, et al. Shifts in the community structure and activity of anaerobic ammonium oxidation bacteria along an estuarine salinity gradient[J]. Journal of Geophysical Research:Biogeosciences, 2016,121(6):1632-1645.
Tomasek A, Kozarek J L, Hondzo M, et al. Environmental drivers of denitrification rates and denitrifying gene abundances in channels and riparian areas[J]. Water Resources Research, 2017,53(8):6523-6538.
李晶莹,韦政.莱州湾海水入侵及土壤盐渍化现状研究[J]. 安徽农业科学, 2010,38(8):4187-4189. Li J Y, Wei Z. Studies on the status of seawater intrusion and soil salinization in Laizhou Bay[J]. Journal of Anhui Agriculture, 2010, 38(8):4187-4189.
张锦峰,高学鲁,李培苗,等.莱州湾西南部海域及其毗邻河流水体营养盐的分布特征及长期变化趋势[J]. 海洋通报, 2015,34(2):222-232. Zhang J F, Gao X L, Li P M, et al. Nutrient distribution characteristics and long-term trends in the southwest of the Laizhou Bay and its adjacent rivers[J]. Marine Science Bulletin, 2015,34(2):222-232.
赵晨英,臧家业,刘军,等.黄渤海氮磷营养盐的分布、收支与生态环境效应[J]. 中国环境科学, 2016,36(7):2115-2127. Zhao C Y, Zang J Y, Liu J, et al. Distribution and budget of nitrogen and phosphorus and their influence on the ecosystem in the Bohai Sea and Yellow Sea[J]. China Environmental Science, 2016,36(7):2115-2127.
Dong L, Nedwell D B, Underwood G J C, et al. Nitrous oxide formation in the Colne estuary in England:the central role of nitrite[J]. Applied and Environmental Microbiology, 2002,68(3):1240-1249.
Dang H, Li J, Chen R, et al. Diversity, abundance, and spatial distribution of sediment ammonia-oxidizing Betaproteobacteria in response to environmental gradients and coastal eutrophication in Jiaozhou Bay, China[J]. Applied and Environmental Microbiology, 2010,76(14):4691-4702.
朱江,周永欣,葛虹,等.离心力对制备沉积物间隙水中化合物浓度的影响[J]. 水生生物学报, 2003,27(5):487-491. Zhu J, Zhou Y X, Ge H, et al. Effect of centrifugal force on compounds concentrations in sediment interstitial water[J]. Acta Hydrobiologica Sinica, 2010,76(14):4691-4702.
Risgaard-Petersen N, Nielsen LP, Rysgaard S, et al. Application of the isotope pairing technique in sediments where anammox and denitrification coexist[J]. Limnology and Oceanography:Methods, 2003,1(1):63-73.
Hallin S, Lindgren PE. PCR detection of genes encoding nitrite reductase in denitrifing bacteria[J]. Applied and Environmental Microbiology, 1999,65(4):1652-1657.
Chon K, Kim Y, Chang NI, et al. Evaluating wastewater stabilizing constructed wetland, through diversity and abundance of the nitrite reductase gene nirS, with regard to nitrogen control[J]. Desalination, 2010,264(3):201-205.
Throbäck I, Enwall K, Jarvis Å, et al.. Reassesing PCR primers targeting nirS, nirK and nosZ genes for community surveys of ammonia oxidizer bacteria with DGGE[J]. FEMS Microbiology Ecology, 2004,49(3):401-417.
Harhangi HR, Le Roy M, van Alen T, et al. Hydrazine synthase, a unique phylomarker with which to study the presence and biodiversity of anammox bacteria[J]. Applied and Environmental Microbiology, 2012,78(3):752-758.
Liu W, Yao L, Jiang X, et al. Sediment denitrification in Yangtze lakes is mainly influenced by environmental conditions but not biological communities[J]. The Science of Total Environment, 2018,616-617:978-987.
Heikkinen RK, Luoto M, Virkkala R, et al. Effects of habitat cover, landscape structure and spatial variables on the abundance of birds in an agricultural-forest mosaic[J]. Journal of Applied Ecology, 2004, 41(5):824-835.
Li J, Yu S, Qin S. Removal capacities and environmental constrains of denitrification and anammox processes in eutrophic riverine sediments[J]. Water, Air, & Soil Pollution, 2020,231(6):274.
王静,刘洪杰,雷禹,等.三小库区小江支流沉积物硝化反硝化速率在蓄水期和泄水期的特征[J]. 环境科学, 2017,38(3):946-953. Wang J, Liu H, Yu L, et al. Nitrification and denitrification rates in a small tributary, Nanhe River, of Three Gorge Dam Reservoir during water collection and release events[J]. Environmental Science, 2017, 38(3):946-953.
吴佳栩,范儒,王敏琛,等.淀山湖沉积物的反硝化脱氮能力及其环境意义[J]. 环境科学学报, 2018,38(3):867-874. Wu J, Fan R, Wang M, et al. Denitrification ability of surficial sediments in Dianshan Lake and its environmental implications[J]. Acta Scientiae Circumstantiae, 2018,38(3):867-874.
Ritz S, Dӓhnke K, Fischer H. Open-channel measurement of denitrification in a large lowland river[J]. Aquatic Sciences, 2018, 80:11.
Zheng Y, Jiang X, Hou L, et al. Shifts in the community structure and activity of anaerobic ammonium oxidation bacteria along an estuarine salinity gradient:shift in anammox along salinity gradient[J]. Journal of Geophysical Research:Biogeosciences, 2016,121(6):1632-1645.
Lisa J A, Song B, Tobias C R, et al. Genetic and biogeochemical investigation of sedimentary nitrogen cycling communities responding to tidal and seasonal dynamics in Cape Fear River Estuary[J]. Estuarine, Coastal and Shelf Science, 2015,167(Part B):A313-A323.
Lu X, Bade D L, Leff L G, et al. The relative importance of anammox and denitrification to total N2 production in Lake Erie[J]. Journal of Great Lakes Research, 2018,44(3):428-435.
杨文焕,石大钧,张元,等.高原湖泊沉积物中反硝化微生物的群落特征[J]. 中国环境科学, 2020,40(1):430-438. Yang W H, Shi D J, Zhang Y, et al. Community characteristics of denitrifying microorganisms in plateau lake sediments-taking Nanhaihu lake as example[J]. China Environmental Science, 2020, 40(1):430-438.
湛钰,高丹丹,盛荣,等.磷差异性调控水稻根际nirK/nirS型反硝化菌组成与丰度[J]. 环境科学, 2019,40(7):3304-3312. Zhan Y, Gao D, Sheng R, et al. Differential responses of rhizospheric nirK-and nirS-type denitrifier communities to different phosphorus levels in paddy soil[J]. Environmental Science, 2019,40(7):3304-3312.
Sun W, Xu M Y, Wu W M, et al.. Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong[J]. Journal of Applied Microbiology, 2014,116(2):464-476.
Wang S, Zhu G, Peng Y, et al. Anammox bacterial abundance, activity, and contribution in riparian sediments of the Pearl River Estuary[J]. Environmental Science & Technology, 2012,46(16):8834-8842.
Dale O R, Tobias C R, Song B. Biogeographical distribution of diverse anaerobic ammonium oxidizing (anammox) bacteria in Cape Fear River Estuary[J]. Environmental Microbiology, 2009,11(5):1194-1207.
Fu B, Liu J, Yang H, et al. Shift of anammox bacterial community structure along the Pearl Estuary and the impact of environmental factors[J]. Journal of Geophysical Research Oceans, 2015,120(4):2869-2883.
Zhou S, Borjigin S, Riya S, et al. The relationship between anammox and denitrification in the sediment of an inland river[J]. Science of the Total Environment, 2014,490(8):1029-1036.
杨京月,郑照明,李军,等.厌氧氨氧化耦合反硝化底物竞争抑制关系特性[J]. 中国环境科学, 2018,38(8):2947-2953. Yang J Y, Zheng Z M, Li J, et al. The inhibitive characteristics by substrate competition of the anammox coupling denitrification process[J]. China Environmental Science, 2018,38(8):2947-2953.
Du R, Peng Y, Ji J, et al. Partial denitrification providing nitrite:opportunities of extending application for anammox[J]. Environment International, 2019,131:105001.
Wang Z, He S, Huang J, et al. Comparison of heterotrophic and autotrophic denitrification processes for nitrate removal from phosphorus-limited surface water[J]. Environment Pollution, 2018, 238:562-572.