Abstract：This study tried to couple the pH value with the reaction kinetics of the denitrification-type methane anaerobic oxidation process. The Denitrifying Anaerobic Methane Oxidation (DAMO) reaction rate and pH value were coupled and evaluated in three reactors with different dominant bacteria. The results show that the Anammox-DAMO maximum denitrification rate and the initial inhibition concentration of nitrate ammonium at 25℃ were 3.95mg/(L×d), 182.63mg/L and 196.40mg/L, respectively. The maximum denitrification rate and the initial inhibition concentration of nitrate in Nitrate-DAMO system were 4.30mg/(L×d) and 367.69mg/L, respectively. The maximum denitrification rate and the initial inhibition concentration of nitrite in Nitrite-DAMO system were 4.04mg/(L×d) and 293.35mg/L, respectively. The denitrification rate of the systems increased first and then decreased with the increase of pH. The optimum pH was 7.5±0.2, 7.2±0.2, 7.8±0.2, respectively. The bacterial growth rate, nitrate affinity constant and inhibition constant of Nitrate-DAMO system were 1291.21cfu/(L×d), 295.23mg/L and 72.63mg/L, respectively; The bacterial growth rate, nitrite affinity constant and inhibition constant of Nitrite-DAMO system were 4040.42cfu/(L×d), 264.51mg/L and 5.02mg/L, respectively. All the three DAMO systems could be described by the coupled Haldane pH model; the denitrification process of Nitrate-DAMO system and Nitrite-DAMO system could also be described by Monod-pH coupling equation.
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