1. School of Water and Environment, Chang'an University, Xi'an 710064, China; 2. Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of the Ministry of Education, Chang'an University, Xi'an 710064, China
Abstract：This study was designed to evaluate performances of the start-up of endogenous partial denitrification and EPD-ANAMMOX process. The glycogen accumulation bacteria (GAO) were enriched in the anaerobic/aerobic sequence batch reactor (SBR) using the activated sludge as inoculum sludge and the acetate acted as carbon source by regulating the influent COD/P ratio as 150:1. Then, the GAOs were induced into denitrifying glycogen accumulation bacteria (DGAO) by means of gradually boosting the concentration of added nitrate at the end of anaerobic phase in SBR. Correspondingly, the level of chemical oxygen demand (COD) at the end of anoxic period was almost the same as that at the distal end of anaerobic stage, the total nitrogen removal efficiency and the average COD removal efficiency were over 98% and 86.74% at the end of anaerobic period of SBR, respectively. Furthermore, the endogenous partial denitrification system was successfully formed by shortening the anaerobic and anoxic time, the ratio of nitrite translated from nitrate (NTR) at the end of anoxic period was up to 65.96%. The average total nitrogen and COD removal load in the 30days operation of the coupled process were 0.222kgN/(m3×d) and 0.337kgCOD/(kgMLVSS.d) with an efficiency of 86.12% and 87.21%, respectively. Typically, the effluent NO3--N concentration was lower than 4.2mg/L and both NO2--N and NH4+-N levels were near to 0mg/L in the same phase. Compared with inoculated sludge, the relative abundance of competibacter increased from 0.001% to 25.06% in the sludge of EPD-SBR during the stable operation period, and the total abundance of Defluviicoccus, Contendobacter, Sphingobium,and Amaricoccus increased from 0.14% to 0.431%, implying that the competibacter was the dominated functional bacteria for the endogenous partial denitrification system.
杨延栋,黄京,韩晓宇,等一体式厌氧氨氧化工艺处理高氨氮污泥消化液的启动[J]. 中国环境科学, 2015,35(4):1082-1087. Yang Y D, Huang J, Han X Y, et al. Start-up of one-stage partial nitrification/anammox process treating ammonium-rich reject water[J]. China Environmental Science, 2015,35(4):1082-1087.
[2]
Jiang H, Yang P, Wang Z, et al. Efficient and advanced nitrogen removal from mature landfill leachate via combining nitritation and denitritation with Anammox in a single sequencing batch biofilm reactor[J]. Bioresource Technology, 2021,333:125138.
[3]
Cao Y S, van Loosdrecht M C M, Daigger G T. Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology, 2017,101(4):1365-1383.
[4]
Du R, Cao S B, Li B K, et al. Performance and microbial community analysis of a novel DEAMOX based on partial-denitrification and anammox treating ammonia and nitrate wastewaters[J]. Water Research, 2017,108:46-56.
[5]
毛佩玥,付雪,赵鑫磊,等.短程反硝化的启动及多参数优化下NO2--N积累特性[J]. 中国环境科学, 2021,41(3):1189-1198. Mao P Y, Fu X, Zhao X L, et al, Start-up of partial denitrification and characteristics of nitrite accumulation by multiple factors[J]. China Environmental Science, 2021,41(3):1189-1198.
[6]
牛萌,王淑莹,杜睿,等.甲醇为碳源短程反硝化亚硝酸盐积累特性[J]. 中国环境科学, 2017,37(9):3301-3308. Niu M, Wang S Y, Du R, et al. Nitrite accumulation properties of partial denitrification with methanol as carbon source[J]. China Environmental Science, 2017,37(9):3301-3308.
[7]
董晓莹,彭党聪.不同碳氮比下污水反硝化过程中亚硝氮积累的特性研究[J]. 环境科学学报, 2017,37(9):3349-3355. Dong X Y, Peng D C, Nitrite accumulation in denitrification with different C/N ratios[J]. Acta Scientiae Circumstantiae, 2017,37(9):3349-3355.
[8]
杜晓娜.反硝化过程中亚硝酸盐积累影响因素与稳定运行[D]. 天津:天津大学, 2016. Du X N, Factors affecting nitrite accumulation and operation stability during biological denitrification[D]. Tian Jin:Tianjin University, 2016.
[9]
田夏迪,茹临锋,吕心涛,等.短程反硝化工艺的研究进展与展望[J]. 中国给水排水, 2020,36(2):7-15. Tian X D, Ru L F, Lv X T, et al. Research progresses and prospect of partial denitrification process[J]. China Water & Wastewater, 2020, 36(2):7-15.
[10]
Ji J T, Peng Y Z, Wang B, et al. Achievement of high nitrite accumulation via endogenous partial denitrification (EPD)[J]. Bioresource Technology, 2017,224:140-146.
[11]
Ji J T, Peng Y Z, Mai W K, et al. Achieving advanced nitrogen removal from low C/N wastewater by combining endogenous partial denitrification with anammox in mainstream treatment[J]. Bioresource Technology, 2018,270:570-579.
[12]
Ji J T, Peng Y Z, Li X Y, et al. Stable long-term operation and high nitrite accumulation of an endogenous partial-denitrification (EPD) granular sludge system under mainstream conditions at low temperature[J]. Bioresource Technology, 2019,289:121634.
[13]
Wang X X, Zhao J, Yu D S, et al. Evaluating the potential for sustaining mainstream anammox by endogenous partial denitrification and phosphorus removal for energy-efficient wastewater treatment[J]. Bioresource Technology, 2019,284:302-314.
[14]
Ji J T, Peng Y Z, Wang B, et al. A novel SNPR process for advanced nitrogen and phosphorus removal from mainstream wastewater based on anammox, endogenous partial-denitrification and denitrifying dephosphatation[J]. Water Research, 2020,170:115363.
[15]
Wu P, Zhang X X, Wang Y G, et al. Development of a novel denitrifying phosphorus removal and partial denitrification anammox (DPR + PDA) process for advanced nitrogen and phosphorus removal from domestic and nitrate wastewaters[J]. Bioresource Technology, 2021,327:124795.
[16]
Wang X X, Zhao J, Yu D S, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019,355:560-571.
[17]
Chu G Y, Yu D S, Wang X X, et al. Comparison of nitrite accumulation performance and microbial community structure in endogenous partial denitrification process with acetate and glucose served as carbon source[J]. Bioresource Technology, 2021,320:124405.
[18]
Ji J T, Peng Y Z, Li X Y, et al, A novel partial nitrification-synchronous anammox and endogenous partial denitrification (PN-SAEPD process for advanced nitrogen removal from municipal wastewater at ambient temperatures[J]. Water Research, 2020,175:115690.
[19]
张超,陈银广.聚糖菌的代谢机制及生物学特性研究进展[J]. 环境污染与防治, 2008,30(8):78-81. Zhang C, Chen Y G, Research advances in the metabolic mechanisms and the microbial characterization of glycogen-accumulating organisms[J]. Environmental Pollution & Control, 2008,30(8):78-81.
[20]
张志剑,周林强,李慧,等.市政污水处理厂生物除磷运行效能与机理分析[J]. 中国环境科学, 2010,30(12):1614-1621. Zhang Z J, Zhou L Q, Li H, et al, Effectivenesses and mechanism of enhanced biological phosphorus removal (EBPR) of municipal wastewater treatment plants[J]. China Environmental Science, 2010, 3030(12):1614-1621.
[21]
国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002:252-354. State Environmental Protection Administration. Monitoring and analysis methods of water and wastewater[M]. Beijing:China Environmental Science Press, 2002:252-354.
[22]
孙艺萍.聚磷菌的筛选及其体内聚磷酸盐推动PHB积累的研究[D]. 哈尔滨:东北林业大学, 2009. Sun Y P, Screening of phosphate-accumulating organisms and study of Polypho sphate promote the PHB accumulation[D]. Haerbin:Northeast Forestry University, 2009.
[23]
鞠洪海.不同电子受体驯化聚糖菌反硝化过程及N2O释放特性[J]. 环境工程, 2020,38(9):113-118. JV H H. Characterics of denitrification and N2O emission of acclimated glycogen accumulating organisms using diferent electron acceptor[J]. Environmental Engineering, 2020,38(9):113-118.
[24]
王景峰,王暄,季民,等.聚糖菌颗粒污泥基于胞内储存物质的同步硝化反硝化[J]. 环境科学, 2006,27(3):473-477. Wang J F, Wang X, Ji M, et al. Intracellular storage polymer driven simultaneous nitrification and denitrification of GAOs granular sludge[J]. Chinese journal of environmental science, 2006,27(3):473-477.
[25]
韦佳敏,刘文如,程洁红,等.反硝化除磷的影响因素及聚磷菌与聚糖菌耦合新工艺的研究进展[J]. 化工进展, 2020,39(11):4608-4618. Wei J M, Liu W R, Cheng J H, et al. Influencing factors of denitrifying phosphorus removal and advance research on novel process of coupling PAOs and GAOs[J]. Chemical Industry and Engineering Progress, 2020,39(11):4608-4618.
[26]
贾淑媛,王淑莹,赵骥,等.驯化后的聚糖菌对NO2--N和NO3--N内源反硝化速率的影响[J]. 化工学报, 2017,68(12):4731-4738. Jia S Y, Wang S Y, Zhao J, et al. Effect of endogenous denitrification rate of domesticated GAOs on NO2--N and NO3--N[J]. CIESC Journal, 2017,68(12):4731-4738.
[27]
Ribera-Guardia A, Marques R, Arangio C, et al. Distinctive denitrifying capabilities lead to differences in N2O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology, 2016, 219:106-113.
[28]
Liu W L, Peng Y Z, Ma B, et al. Dynamics of microbial activities and community structures in activated sludge under aerobic starvation[J]. Bioresource Technology, 2017,244:588-596.
[29]
李叶青,景张牧,江皓,等.微生物组学及其在厌氧消化中的研究进展[J]. 生物技术通报, 2021,37(1):90-101. Li Y Q, Jing Z M, Jiang H, et al. Microbiome and its research progress of anaerobic digestion[J]. Biotechnology Bulletin, 2021,37(1):90-101.
[30]
李蕾,何琴,马垚,等.厌氧消化过程稳定性与微生物群落的相关性[J]. 中国环境科学, 2016,36(11):3397-3404. Li L, He Q, Ma Y, et al. Investigation on the relationship between process stability and microbial community in anaerobic digestion[J]. China Environmental Science, 2016,36(11):3397-3404.
[31]
Oehmen A, Carvalho G, Lopez-Vazquez C M, et al. Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Water Research, 2010,44(17):4992-5004.
[32]
常烁,曾薇.EBPR系统中聚糖菌及其反硝化代谢机理的研究进展[J]. 工业水处理, 2019,39(9):8-13. Chang S, Zeng W. Glycogen accumulating organisms and its denitrifying metabolic mechanism in enhanced biological phosphorus removal systems:a review[J]. Industrial Water Treatment, 2019,39(9):8-13.
[33]
Zhao W H, Peng Y Z, Wang M X, et al. Nutrient removal and microbial community structure variation in the two-sludge system treating low carbon/nitrogen domestic wastewater[J]. Bioresource Technology, 2019,294:122161.
[34]
Roy S, Nirakar P, Yong N G H, et al. Denitrification kinetics indicates nitrous oxide uptake is unaffected by electron competition in Accumulibacter[J]. Water Research, 2021,189:116557.
[35]
于莉芳,汪宇,滑思思,等.城市污水处理厂进水氨氧化菌对活性污泥系统的季节性影响[J]. 环境科学, 2021,42(4):1923-1929. Yu L F, Wang Y, Hua S S, et al. Seasonal effects of influent ammonia oxidizing bacteria of municipal wastewater treatment plants on activated sludge system[J]. Environmental Science, 2021,42(4):1923-1929.
[36]
史文燕,张健.硝化杆菌(Nitrobacte)和硝化螺菌(Nitrospira)在脱氮系统中的研究进展[J]. 应用化工, 2020,49(10):2581-2585. Shi W Y, Zhang J. Research progress of nitrobacter and nitrospira in the nitrogen removal system[J]. Applied Chemical Industry, 2020, 49(10):2581-2585.
[37]
成敏.高效除磷活性污泥中功能菌解析及其除磷基因组学基础研究[D]. 西安:西安建筑科技大学, 2018. Cheng M. Metagenomic analysis of functional bacteria and their phosphorus-removing function of activated sludge in enhanced biological phosphorus removal[D]. Xi'an:XI'AN University of Architecture and Technology, 2018.
[38]
高大文,辛晓东.MBR膜污染过程中微生物群落结构与代谢产物分析[J]. 哈尔滨工业大学学报, 2014,46(2):26-32. Gao D W, Xin X D. Analysis of microbial community structure and metabolites during the MBR membrane fouling process[J]. Journal of Harbin Institute of Technology, 2014,46(2):26-32.
