Functions of H2S signal in response to SO2 stress in Arabidopsis thaliana
LI Li-hong1, GUO Yu-ru1, HOU Jun-xin1, WU Li-hua2
1. Department of Chemistry and Chemical Engineering, Jinzhong University, Yuci 030619, China; 2. Department of Biology, Taiyuan Normal University, Yuci 030619, China
Abstract：Sulfur dioxide (SO2) is one of the most common and harmful air pollutant. Hydrogen sulfide (H2S), as a new gasotransmitter, is involved in the regulation of plant development and stress adaptation. To reveal the role of H2S in response to SO2 stress, the relation between transcriptional response to H2S and SO2 treatments, and the production and physiological effect of H2S during SO2 fumigation will be studied in Arabidopsis plants. By using high-throughput sequencing, many genes were identified to be differentially expressed in Arabidopsis plants exposed to 30mg/m3 SO2 or treated with 0.1mmol/L H2S. Among these differentially expressed genes (DEGs), 122, 0genes were overlapped between SO2 and H2S treatments, including some genes encoding enzymes involved in sulfur and glutathione metabolism. These results suggested a interaction between H2S and SO2 in regulating sulfur metabolism pathways in plants. Moreover, two H2S-generating genes, L-cysteine desulfhydrase (LCD) and L-cysteinedesulfhydrase1 (DES1) were significantly up-regulated, and endogenous H2S content was enhanced in Arabidopsis plants under SO2 stress. Meanwhile, the activities of superoxide dismutase (SOD) and catalase (CAT), the content of glutathione (GSH) and activities of GSH-related enzymes including glutathione S-transferase (GST) and glutathione peroxidase (GPX) were significantly increased in Arabidopsis plants exposed to SO2. And that H2O2 content was markedly increased in SO2-treated Arabidopsis plants, while malondialdehyde (MDA) content showed no significant difference compared with the control group. Exogenous application of H2S further increased the level of GSH and GSH-related defense enzyme, and reduced the content of H2O2 in Arabidopsis plants under SO2 stress. When scavenged endogenous H2S by spraying hypotaurine (HT) during SO2 fumigation, the content of GSH, the activities of SOD, CAT and GST were reduced, and the content of MDA was significantly increased in Arabidopsis plants. Together, this study indicated that H2S played an important role in response to SO2 stress through improving antioxidant capacity, which would be helpful for better understanding the adaptation mechanism of plants to environmental stress.
李利红, 郭宇茹, 侯俊鑫, 吴丽华. H2S信号在拟南芥响应SO2胁迫过程中的作用[J]. 中国环境科学, 2022, 42(6): 2904-2910.
LI Li-hong, GUO Yu-ru, HOU Jun-xin, WU Li-hua. Functions of H2S signal in response to SO2 stress in Arabidopsis thaliana. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2904-2910.
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