为探究微塑料(MPs)对活性污泥脱氮性能的尺寸影响效应,采用水质测定、活性测试、物化表征及微生物群落分析等方法,探索了不同粒径下(75~500μm)聚对苯二甲酸乙二醇酯(PET)对厌氧氨氧化颗粒污泥(AnGS)的影响.结果显示,投加75和150μm的PET-MPs时,Anammox脱氮效能低于控制组,而300和500μm组中却表现出优于控制组.厌氧氨氧化活性(SAA)测试具有相似结果.胞外聚合物(EPS)分析发现PET-MPs粒径越小,EPS分泌和PN/PS越高,AnGS结构越不完整.小粒径下PET对Anammox抑制主要与颗粒污泥所具有的较低Zeta电位和较小的比表面积显著相关.而大粒径PET-MPs则减少了与MPs接触的机率且能提供更多的活性位点,促进了Anammox底物的传递效率.高通量测序结果表明PET-MPs粒径大小决定了微生物群落的多样性和丰富度,尤其对Anammox菌属(Candidatus Kuenenia)相对丰度影响显著.
Abstract
To investigate the size-dependent effect of microplastics (MPs) on nitrogen removal performance of activated sludge, the effect of polyethylene terephthalate (PET) on anaerobic ammonia-oxidizing granular sludge (AnGS) at different particle sizes (75~500μm) was explored using the methods of water quality determination, activity test, physicochemical characterization and microbial community analysis in this study. Results showed that the nitrogen removal efficiency of Anammox was lower than that of the control group when 75 and 150μm PET-MPs were dosed, while the 300 and 500μm groups showed superiority over the control group. Anaerobic ammonia oxidation activity (SAA) tests showed similar results. Extracellular polymeric substances (EPS) analysis revealed that the smaller the particle size of PET-MPs, the higher the EPS secretion and PN/PS, and the more incomplete the AnGS structure. The inhibition of Anammox by PET at small particle size was mainly associated with the granular sludge's lower Zeta potential and smaller specific surface area. In contrast, large-size PET-MPs reduced the chance of contact with MPs and provided more active sites, facilitating Anammox substrates' delivery efficiency. High-throughput sequencing results showed that the particle size of PET-MPs determined the diversity and abundance of microbial community, especially the relative abundance of Candidatus Kuenenia.
关键词
聚对苯二甲酸乙二醇酯 /
生物脱氮 /
微生物群落 /
微塑料 /
厌氧氨氧化 /
影响
Key words
anammox /
biological nitrogen removal /
effect /
microbial community /
microplastics /
PET
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基金
国家自然科学基金资助项目(21607111);山西省基础研究计划项目(20210302123198)
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